Multicolor Imaging Based on Interferometric Information in 4Pi Single-Molecule Switching Nanoscopy

By utilizing a dual-objective setup in single-molecule switching nanoscopy (SMSN; e.g. PALM, STORM or FPALM), it is possible to obtain three-dimensional (3D) images at 10 to 20 nm resolution. We have recently expanded this technology to the imaging of thick cells through the addition of deformable mirrors in each arm of the 4Pi-interferometric cavity and new image processing algorithms, significantly increasing the application range.

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