Influenza vaccine effectiveness against hospitalisation with influenza in adults in Australia in 2014

Publication date: Available online 31 October 2015
Source:Vaccine
Author(s): Allen C. Cheng, Tom Kotsimbos, Paul M. Kelly
We provide estimates of the influenza vaccine protection against hospitalisation with laboratory-confirmed influenza in the 2014 Australian season where the A/H1N1/pdm09 strain predominated. This was performed using a case-test negative study design as part of a national sentinel surveillance system in Australia. Vaccine effectiveness was estimated as (1-OR)×100% where the odds ratio of vaccination in cases vs test negative participants was estimated from a conditional logistic regression. Between April and November, 1692 adult patients were admitted with laboratory-confirmed influenza. Vaccine effectiveness was estimated from 1283 patients with influenza and 1116 test negative patients where vaccination status was ascertained. Vaccination was associated with a reduction in the risk of hospitalisation with influenza of 51.5% (95% CI: 41.6%, 59.7%) in all patients, and a reduction of 50.7% (95% CI: 40.1%, 59.3%) in the target population for vaccination. We estimate that the influenza vaccine was moderately protective against hospitalisation with laboratory-confirmed influenza during the 2014 influenza season in Australia.



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A new EV71 VP3 epitope in norovirus P particle vector displays neutralizing activity and protection in vivo in mice

Publication date: Available online 31 October 2015
Source:Vaccine
Author(s): Liping Jiang, Rongjun Fan, Shiyang Sun, Peihu Fan, Weiheng Su, Yan Zhou, Feng Gao, Fei Xu, Wei Kong, Chunlai Jiang
Enterovirus 71 (EV71) and Coxsackievirus A16 (CVA16), as the main agents causing hand, foot and mouth disease (HFMD), have become a serious public health concern in the Asia-Pacific region. Recently, various neutralizing B cell epitopes of EV71 were identified as targets for promising vaccine candidates. Structural studies of Picornaviridae indicated that potent immunodominant epitopes typically lie in the hypervariable loop of capsid surfaces. However, cross-neutralizing antibodies and cross-protection between EV71 and CVA16 have not been observed. Therefore, we speculated that divergent sequences of the two viruses are key epitopes for inducing protective neutralizing responses. In this study, we selected 10 divergent epitope candidates based on alignment of the EV71 and CVA16 P1 amino acid sequences using the Multalin interface page, and these epitopes are conserved among all subgenotypes of EV71. Simultaneously, by utilizing the norovirus P particle as a novel vaccine delivery carrier, we identified the 71-6 epitope (amino acid 176–190 of VP3) as a conformational neutralizing epitope against EV71 in an in vitro micro-neutralization assay as well as an in vivo protection assay in mice. Altogether, these results indicated that the incorporation of the 71-6 epitope into the norovirus P domain can provide a promising candidate for an effective synthetic peptide-based vaccine against EV71.



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Pros and cons of VP1-specific maternal IgG for the protection of Enterovirus 71 infection

Publication date: Available online 31 October 2015
Source:Vaccine
Author(s): Young-In Kim, Jae-Hyoung Song, Bo-Eun Kwon, Ha-Neul Kim, Min-Duk Seo, KwiSung Park, SangWon Lee, Sang-Gu Yeo, Mi-Na Kweon, Hyun-Jeong Ko, Sun-Young Chang
Enterovirus 71 (EV71) causes hand, foot, and mouth diseases and can result in severe neurological disorders when it infects the central nervous system. Thus, there is a need for the development of effective vaccines against EV71 infection. Here we report that viral capsid protein 1 (VP1), one of the main capsid proteins of EV71, efficiently elicited VP1-specific immunoglobulin G (IgG) in the serum of mice immunized with recombinant VP1. The VP1-specific IgG produced in female mice was efficiently transferred to their offspring, conferring protection against EV71 infection immediately after birth. VP1-specific antibody can neutralize EV71 infection and protect host cells. VP1-specific maternal IgG in offspring was maintained for over 6 months. However, the pre-existence of VP1-specific maternal IgG interfered with the production of VP1-specific IgG antibody secreting cells by active immunization in offspring. Therefore, although our results showed the potential for VP1-specific maternal IgG protection against EV71 in neonatal mice, other strategies must be developed to overcome the hindrance of maternal IgG in active immunization. In this study, we developed an effective and feasible animal model to evaluate the protective efficacy of humoral immunity against EV71 infection using a maternal immunity concept.



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Differences in efficiency, satisfaction and adverse events between self-administered intradermal and nurse-administered intramuscular influenza vaccines in hospital workers

Publication date: Available online 31 October 2015
Source:Vaccine
Author(s): Brenda L. Coleman, Shelly A. McNeil, Joanne M. Langley, Scott A. Halperin, Allison J. McGeer
Vaccinating healthcare workers against influenza takes tens of thousands of hours of work annually. This study was undertaken to determine the acceptability, success rate, and time to vaccinate healthcare workers in nurse-led groups that self-vaccinated with intradermal influenza vaccine compared with nurse-administered intramuscular vaccine.MethodsVolunteer hospital workers were randomly assigned to groups that either self-administered intradermal influenza vaccine (Intanza^®) in a nurse-led group or received nurse-administered intramuscular vaccine (Vaxigrip^®). Research assistants timed vaccination procedures; pre- and post-injection questionnaires assessed acceptability and reactogenicity.Results810 adults, 21–69 years of age, from two study sites were vaccinated: 401 self-administered the intradermal vaccine while 409 received their intramuscular vaccine from a nurse. Of those who self-administered for the first time, 98.5% were successful on their first attempt with an additional 1.5% on their second attempt. Acceptability was high: 96% were very or somewhat certain that they administered the vaccine correctly, 83% would choose intradermal influenza vaccine again and of those, 75% would choose self-administration again, if given the choice. It took 51.3–72.6s per person for the nurses to guide the groups through the self-administration process, which was significantly less time than it took to individually administer the intramuscular vaccines (93.6s).ConclusionSelf-administration of intradermal influenza vaccine by people working in healthcare settings is a possible alternative to nurse administered vaccinations, with nurse-led group sessions a good way of teaching the technique while being available to respond to unanticipated problems (NCT01665807).



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Pertussis vaccination during pregnancy in Vietnam: Results of a randomized controlled trial Pertussis vaccination during pregnancy

Publication date: Available online 31 October 2015
Source:Vaccine
Author(s): Hoang Thi Thu Ha, Elke Leuridan, Kirsten Maertens, Trung Dac Nguyen, Niel Hens, Ngoc Ha Vu, Raissa Nadège Caboré, Hong Thi Duong, Kris Huygen, Pierre Van Damme, Dang Duc Anh
A pertussis vaccination during pregnancy has recently been adopted in several countries to indirectly protect young infants. This study assessed the effect of adding a pertussis component to the tetanus vaccination, in the pregnancy immunization program in Vietnam.A randomized controlled trial was performed. Pregnant women received either a Tdap (tetanus, diphtheria acellular pertussis) vaccine or a tetanus only vaccine between 19 and 35 weeks' gestational age. Immunoglobulin G (IgG) against tetanus (TT), diphtheria (DT), pertussis toxin (PT), filamentous hemaglutinin (FHA) and pertactin (Prn) were measured using commercial ELISA tests, at baseline, 1 month after maternal vaccination, at delivery, and in infants from cord blood and before and after the primary series (EPI: month 2-3-4) of a pertussis containing vaccine.Significantly higher geometric mean concentrations (GMC) were observed for all 3 measured pertussis antigens in the offspring of the Tdap group, up to 2 months of age. One month after completion of the primary infant vaccination schedule, anti-Prn GMC, but not anti-PT and anti-FHA GMCs, was significantly (p=0.006) higher in the control group.Maternal antibodies induced by vaccination during pregnancy close the susceptibility gap for pertussis in young infants. Limited interference with the infant vaccine responses was observed. Whether this interference effect disappears with the administration of a fourth vaccine dose is further studied.



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PneuMum: Impact from a randomised controlled trial of maternal 23-valent pneumococcal polysaccharide vaccination on middle ear disease amongst Indigenous infants, Northern Territory, Australia

Publication date: Available online 31 October 2015
Source:Vaccine
Author(s): Michael J. Binks, Sarah A. Moberley, Anne Balloch, Amanda J. Leach, Sandra Nelson, Kim M. Hare, Cate Wilson, Peter S. Morris, Jane Nelson, Mark D. Chatfield, Mimi L.K. Tang, Paul Torzillo, Jonathan R. Carapetis, E. Kim Mulholland, Ross M. Andrews
BackgroundWe assessed maternal 23-valent pneumococcal polysaccharide (23vPPV) vaccine efficacy (VE) against middle ear disease and pneumococcal carriage amongst Australian Indigenous infants.MethodsIn an open label, allocation concealed, outcome-assessor blinded, community stratified, randomised controlled trial, healthy pregnant Indigenous women aged 17–39 years in the Northern Territory of Australia received the 23vPPV (1:1:1) at: 30–36 weeks gestation, birth, or were unvaccinated (ClinicalTrials.gov NCT00714064). Co-primary outcomes were the point prevalences of infant middle ear disease and 23vPPV-type carriage at age 7 months.ResultsThe consent rate was 50% (313/632). Among 227 eligible participants randomised, retention rates were 86% (66/77) controls; 89% (67/75) pregnancy vaccinees; 88% (66/75) birth vaccinees. At infant age 7 months, ear disease prevalence was: 71% (47/66) controls, 63% (42/67) pregnancy vaccinees, 76% (50/66) birth vaccinees; and 23vPPV-type carriage was: 26% (17/66) controls, 18% (12/67) pregnancy vaccinees, 18% (12/66) birth vaccinees. For pregnancy vaccinees, VE was 12% (95% CI −12% to 31%) against infant ear disease and 30% (95% CI −34% to 64%) against 23vPPV-type carriage. In a post-hoc analysis, VE against infant ear disease concurrent with carriage of 23vPPV or related types was 51% (95% CI −2% to 76%). There were no serious adverse effects following receipt of the 23vPPV in pregnancy or at birth.ConclusionsIn a high risk population, our study was unable to demonstrate efficacy of 23vPPV in pregnancy against the co-primary outcomes of either all-cause infant ear disease or 23vPPV-type nasopharyngeal carriage at age 7 months. Efficacy against ear disease concurrent with carriage of vaccine-related serotypes (a more specific outcome) suggests 23vPPV in pregnancy may complement childhood pneumococcal vaccination programs.



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Translating Research to Practice for Children With Autism Spectrum Disorder: Part I: Definition, Associated Behaviors, Prevalence, Diagnostic Process, and Interventions

The number of children with autism spectrum disorder (ASD) is rising, along with the potential for challenging behaviors during health care encounters.

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Testing anti-HIV activity of antiretroviral agents in vitro using flow cytometry analysis of CEM-GFP cells infected with transfection-derived HIV-1 NL4-3

ABSTRACT

An assay, specifically optimized to evaluate the anti-HIV activity of antiretrovirals by flow cytometry analysis, is described. As widely used anti-HIV agents, zidovudine (AZT), abacavir (ABC), 2',3'-dideoxyinosine (DDI), lamivudine (3TC), nevirapine (NVP), and efavirenz (EFV), and as drugs of recent approval raltegravir (RAL), etravirine (ETR) and rilpivirine (RPV), were utilized as reference drugs. HIV-1 NL4-3 virus was prepared by transfection of HEK293T cells with purified plasmid DNA and quantified by p24 antigen-capture assay. For infection, CEM-GFP cells were exposed to vehicle or to several concentrations of the drugs for 2 h at 37°C before HIV-1 NL4-3 was added to each sample. The adsorption was prolonged for 3 h at 37°C. After 72 h incubation, HIV-induced GFP expression in infected CEM-GFP cells was assessed by flow cytometry analysis and expressed as % positive cells. For comparison, p24 production in supernatants was assessed by a commercial ELISA kit. On the basis of IC₅₀ values, the anti-HIV activity, as assayed by this method, was EFV > 3TC > AZT > NVP > DDI > ABC and ETR > RPV > RAL. The comparison between the IC₅₀ values calculated through flow cytometry and p24 production revealed overlapping results, showing that the optimized protocol of CEM-GFP infection with HIV NL4-3 is a suitable method to perform quantitative, rapid and low-expensive screening tests to evaluate the in vitro effect of new candidate anti-HIV drugs. This article is protected by copyright. All rights reserved

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Risk of congenital cytomegalovirus infection among HIV-exposed uninfected infants is not decreased by maternal nelfinavir use during pregnancy

Abstract

Background

Congenital cytomegalovirus (cCMV) infection is common among infants born to HIV-infected women. Nelfinavir (NFV), an antiretroviral drug that is safe during pregnancy, inhibits CMV replication in vitro at concentrations that standard doses achieve in plasma. We hypothesized that infants born to women receiving NFV for prevention of mother-to-child transmission of HIV (PMTCT) would have a reduced prevalence of cCMV infection.

Methods

The prevalence of cCMV infection was compared among HIV-uninfected infants whose HIV-infected mothers either received NFV for >4 weeks during pregnancy (NFV-exposed) or did not receive any NFV in pregnancy (NFV-unexposed). CMV PCR was performed on infant blood samples collected at <3 weeks from birth.

Results

Of the 1,255 women included, 314 received NFV for >4 weeks during pregnancy and 941 did not receive any NFV during pregnancy. The overall prevalence of cCMV infection in the infants was 2.2%, which did not differ by maternal NFV use. Maternal CD4 T cell counts were inversely correlated with risk of cCMV infection, independent of the time NFV was initiated during gestation. Infants with cCMV infection were born 0.7 weeks earlier (p = 0.010) and weighed 170 grams less (p = 0.009) than uninfected infants.

Conclusion

Among HIV-exposed uninfected infants, cCMV infection was associated with adverse perinatal outcomes. NFV use in pregnancy was not associated with protection against cCMV. Safe and effective strategies to prevent cCMV infection are needed. This article is protected by copyright. All rights reserved

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Susceptibility of HPV16 and 18 to high level disinfectants indicated for semi-critical ultrasound probes

Abstract

Background

Ultrasound probes used in endocavitary procedures have been shown to be contaminated with high-risk HPV after routine use and HPV is also known to be resistant to some high level disinfectants (HLDs). This study compared efficacy of two leading ultrasound probe HLD methods; liquid ortho-phthalaldehyde (Cidex® OPA) and an automated device using sonicated hydrogen peroxide (trophon® EPR) against HPV16 and HPV18 in a hard-surface carrier test.

Methods

Native HPV16 and HPV18 virions were generated in organotypic epithelial raft cultures. Viral lysates were dried onto carriers with a 5% (v/v) protein soil. Efficacy tests were performed against the automated device at 35% and 31.5% H₂O₂ and 0.55% OPA in quadruplicate with matched input, neutralization and cytotoxicity controls. Hypochlorite was included as a positive control. Infectivity was determined by the abundance (qRT-PCR) of the spliced E1^E4 transcript in infected recipient cells.

Results

The automated HLD device showed excellent efficacy against HPV16 and HPV18 (>5 log₁₀ reductions in infectivity) whereas OPA showed minimal efficacy (<0.6 log₁₀ reductions).

Conclusions

While HPV is highly resistant to OPA, sonicated hydrogen peroxide offers an effective disinfection solution for ultrasound probes. Disinfection methods that are effective against HPV should be adopted where possible. This article is protected by copyright. All rights reserved

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Increased expression and dysregulated association of restriction factors and type I interferon in HIV, HCV mono- and co-infected patients

Abstract

Host restriction factors and type I interferon are important in limiting HIV and HCV infections, yet the role of HIV, HCV mono- and co-infection in regulating these antiviral genes expression is not clear. In this study, we measured the levels of TRIM5α, TRIM22, APOBEC3G, and IFN-α, -β mRNA expression in peripheral blood mononuclear cells of 43 HIV mono-infected, 70 HCV mono-infected and 64 HIV/HCV co-infected patients along with 98 healthy controls. We also quantified HIV and HCV viral loads in mono- and co-infected patients. The results showed that HCV, HIV mono- and co-infection differentially increased TRIM22, APOBEC3G and IFN-α, -β mRNA expression while the mRNA expression of TRIM5 was upregulated only by HCV-mono infection. HIV/HCV co-infection was associated with higher viral load, compared to either HIV or HCV mono-infection. Additionally, we showed TRIM5 and TRIM22 positively correlated with IFN-α, -β, which could be dysregulated by HIV, HCV mono- and co-infection. Furthermore, we found TRIM22 negatively correlated with HCV viral load in mono-infected patients and APOBEC3G positively correlated with HIV viral load in co-infected patients. Collectively, our findings suggest the potential role of restriction factors in restricting HIV, HCV mono- and co-infection in vivo, which appears to be a therapeutic target for potential drug discovery. This article is protected by copyright. All rights reserved

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Human papillomavirus infection in male patients with STI-related symptoms in Hanoi, Vietnam

Abstract

This cross-sectional study investigated the prevalence, genotypes, and risk factors for human papillomavirus (HPV) infection in Hanoi, Vietnam. The study included 192 males (mean age, 32.9 years) with symptoms related to sexually transmitted infections (STI). Urinary, penile, and urethral samples were collected in April and May, 2014. HPV DNA was detected with PCR, performed with modified and/or original GP5⁺/GP6⁺ primers. HPV genotypes were determined with a gene array assay. Neisseria gonorrhoeae (NG) and Chlamydia trachomatis (CT) DNA were detected with loop-mediated isothermal amplification. HPV DNA, NG, and CT were detected in 48 (25.0%), 23 (12.0%), and 41 (21.4%) patients, respectively. HPV DNA appeared in penile samples (21.0%, 39/186) more frequently than in urinary (3.1%, 6/191, p < 0.001) and urethral (9.4%, 18/192, p = 0.002) samples. Among patients with HPV, genotype prevalence was: HPV81 (22.9%), HPV52 (18.8%), HPV18 (16.7%), and HPV16 (6.3%). Multiple-type and high risk-type HPV infections were determined in 33.3% and 64.6%, respectively. Maltivariate analysis showed a significant association of HPV infection in urethra with younger sexual debut age. HPV52 was the most prevalent high-risk HPV genotype, whereas HPV16 was less common in the male Vietnamese patients with STI-related symptoms. Younger sexual-debut age was a risk factor for HPV infection in urethra. This article is protected by copyright. All rights reserved

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Hydrolyzed fish proteins reduced activation of caspase-3 in H 2 O 2 induced oxidative stressed liver cells isolated from Atlantic salmon (Salmo salar)

Hydrolyzed fish proteins (H-pro) contains high concentrations of free amino acids and low molecular peptides that potentially benefit health. The following study aimed to test whether the water soluble phase of H-pro could reduce apoptosis and inflammation in primary liver cells isolated from Atlantic salmon following H 2 O 2 provoked oxidative stress. Cells were grown as monocultures or co-cultured with head kidney cells to assess possible cross talk in inflammation and metabolism during treatments. Cells were grown in media with or without H-pro for 2 days before being stressed with 200 µM H 2 O 2 then harvested 24 h post exposure. Both treatments were compared to the respective treatments without H 2 O 2 supplementation. Oxidative stressed cells had increased activation of caspase-3, but supplementation with H-pro in the media prior to the oxidative stress reduced caspase-3 activation. In conclusion, free amino acids and low molecular weight peptides from H-pro attenuated oxidative stress, and made cells able to withstand apoptosis after H 2 O 2 provoked oxidative stress.

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Contaminant release history identification in 2-D heterogeneous aquifers through a minimum relative entropy approach

The minimum relative entropy (MRE) method has been applied in a wide variety of fields since it was first introduced. Woodbury and Ulrych (Water Resour Res 29(8): 2847–2860, 1993 , Water Resour Res 32(9): 2671–2681, 1996 ) adopted and improved this method to solve linear inverse problems in aquifers. In this work, the MRE method was improved to detect the source release history in 2-D aquifer characterized by a non-uniform flow-field. The approach was tested on two cases: a 2-D homogeneous conductivity field and a heterogeneous one (the hydraulic conductivity presents three orders of magnitude in terms of variability). In the latter case the transfer function cannot be described with an analytical formulation, thus, the transfer functions were estimated by means of a numerical procedure. In order to analyze the method performance in different conditions, two datasets have been used: observations collected at the same time at 20 different monitoring points, and observations collected at 2 monitoring points at several times. The observed data have been processed with and without a random error and the Boxcar and Gaussian probability distribution functions were considered as a priori information. The agreement between the true and the estimated data has been evaluated through the calculation of the normalized Root Mean Square error. The approach was able to recover the release history even in the most difficult case.

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Medicine,New Articles,October 31st,2015

Synthesis and comparison of the biological activity of monocyclic phosphonate, difluorophosphonate and phosphate analogs of the natural AChE inhibitor cyclophostin

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 δευτερόλεπτα

Publication date: Available online 30 October 2015 *Source:*Bioorganic &amp; Medicinal Chemistry Author(s): Benjamin P. Martin, Elena Vasilieva, Cynthia M. Dupureur, Christopher D. Spilling New monocyclic phosphate, phosphonate and difluorophosphonate analogs of the natural AChE inhibitor cyclophostin were synthesized and their activity toward human AChE examined. Surprisingly, the phosphate, phosphonate, and difluorophosphonate analogs all showed diminished activity when compared with the natural product. Graphical abstract [image: image] from #Medicine via ola Kala on Inoreader http:...περισσότερα »

1,2,3-Triazole pharmacophore-based benzofused nitrogen/sulfur heterocycles with potential anti-Moraxella catarrhalis activity

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 δευτερόλεπτα

Publication date: Available online 30 October 2015 *Source:*Bioorganic &amp; Medicinal Chemistry Author(s): Silvija Maračić, Tatjana Gazivoda Kraljević, Hana Čipčić Paljetak, Mihaela Perić, Mario Matijašić, Donatella Verbanac, Mario Cetina, Silvana Raić-Malić Versatile 1,2,3-triazole pharmacophore-based benzofused heterocycles containing halogen-substituted aromatic (9–17 and 25–28), 7-substituted coumarin (18–23 and 29–30) or penciclovir-like subunit (31a,b–38a) were designed and synthesized to evaluate their antibacterial activities against selected Gram-positive and Gram-negative bac... περισσότερα »

Discovery of bis-ary urea derivatives as potent and selective Limk inhibitors: Exploring Limk1 activity and Limk1/ROCK2 selectivity through a combined computational study

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 δευτερόλεπτα

Publication date: Available online 30 October 2015 *Source:*Bioorganic &amp; Medicinal Chemistry Author(s): Jiaxin Cui, Mei Ding, Wei Deng, Yan Yin, Zhonghua Wang, Hong Zhou, Guofeng Sun, Yu Jiang, Yangbo Feng Lim kinase (Limk), a proline/serine-rich sequence, can regulate the polymerization of the actin filaments by phosphorylating, and it is found to be highly involved in various human diseases. In this paper, 47 reported Limk1 inhibitors with bis-ary urea scaffold were used to design potent and selective Limk inhibitors by computational approaches. Firstly, the structure-Limk1 activi... περισσότερα »

Synthesis of di- and tri-saccharide fragments of Salmonella typhi Vi capsular polysaccharide and their zwitterionic analogues

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 34 δευτερόλεπτα

Publication date: Available online 30 October 2015 *Source:*Bioorganic &amp; Medicinal Chemistry Author(s): Matteo Fusari, Silvia Fallarini, Grazia Lombardi, Luigi Lay Zwitterionic polysaccharides (ZPS) behave like traditional T cell-dependent antigens, suggesting the design of new classes of vaccines alternative to currently used glycoconjugates and based on the artificial introduction of a zwitterionic charge motif onto the carbohydrate structure of pathogen antigens. Here we report the new synthesis and antigenic evaluation of di-/tri-saccharide fragments of Salmonella typhi Vi polysa...περισσότερα »

Investigation of the mechanisms of Angelica dahurica root extract-induced vasorelaxation in isolated rat aortic rings

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 1 ώρα

Background: The root of Angelica dahurica Bentham et Hooker (Umbelliferae) has been used as a traditional medicine for colds, headache, dizziness, toothache, supraorbital pain, nasal congestion, acne, ulcer, carbuncle, and rheumatism in China, Japan, and Korea. Interestingly, it has been used in the treatment of vascular diseases including hypertension. The aim of this study was to provide pharmacological evidence for the anti-hypertensive effect of A. dahurica by investigating the mechanism underlying its vasorelaxant effect. Methods: The vasorelaxant effects of a 70 % methanol e... περισσότερα »

Retrospective case-series of Paecilomyces lilacinus ocular mycoses in Queensland, Australia

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 1 ώρα

Background: The purpose of this study was to report: (1) the varying presentation of Paecilomyces ocular infections arising in Queensland; (2) the significance of immunosuppression as a primary determinant of disease; (3) the outcomes of voriconazole use; and (4) the ongoing need for both surgical and medical management of this devastating fungal infection. Methods: A retrospective case series of 21 culture proven individuals participated in this series and were identified via a review of the pathology reporting system utilized in the Queensland public health system. All culture p... περισσότερα »

Meningitis caused by Pasteurella multocida in a dog owner without a dog bite: clonal lineage identification by MALDI-TOF mass spectrometry

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 1 ώρα

Background: Pasteurella multocida meningitis in an immunocompetent patient is rare and commonly occurs after animal bite. To our knowledge, only 48 cases have been reported in the literature since 1989. P. multocida meningitis is commonly linked to animal contagion. Here we report on a new case of P. multocida meningitis in an immunocompetent patient who is a dog owner without a dog bite. We used the matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry to investigate the clonal lineage between animal and human isolates.Case presentationIn our ca...περισσότερα »

Effect of consuming novel foods consisting high oleic canola oil, barley β-glucan, and DHA on cardiovascular disease risk in humans: the CONFIDENCE (Canola Oil and Fibre with DHA Enhanced) study – protocol for a randomized controlled trial

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

Background: Metabolic syndrome (MetS) has been identified as a major contributor to the development of cardiovascular disease (CVD). Current recommendations for dietary management of people with MetS involve quantitative and qualitative modifications of food intake, such as high consumption of vegetables, fruits, and whole grain foods. The results from our previous human trials revealed the potential of the dietary components high-oleic acid canola oil (HOCO)-docosahexaenoic acid (DHA) and high molecular weight barley β-glucan individually in managing CVD risk factors. Foods with ... περισσότερα »

Analysis of the Cochrane Review: Interventions for Improving Upper Limb Function after Stroke. Cochrane Database Syst Rev. 2014,11:CD010820.

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

Impairment of the upper limbs is quite frequent after stroke, making rehabilitation an essential step towards clinical recovery and patient empowerment. This review aimed to synthetize existing evidence regarding interventions for upper limb function improvement after Stroke and to assess which would bring some benefit. The Cochrane Database of Systematic Reviews, the Database of Reviews of Effects and PROSPERO databases were searched until June 2013 and 40 reviews have been included, covering 503 studies, 18 078 participants and 18 interventions, as well asdifferent doses and set... περισσότερα »

Hyperbaric Oxygen Therapy for the Treatment of Fournier’s Gangrene: A Review of 34 Cases

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

*Introduction:* Fournier's gangrene is a serious necrotizing infection that can be fatal if not promptly attended. Treatment for this condition consists of a combination of surgical debridement, antibiotherapy and supportive care. Hyperbaric oxygen therapy is used as an adjuvant for the optimization of infected tissue oxygenation and for its bactericidal and bacteriostatic effects. *Material and Methods:* The data presented in this study encompass a period of 25 years of clinical records of patients with Fournier's gangrene that had been treated at our center with hyperbaric oxygen... περισσότερα »

Alcohol and Youth in Portugal: An Intervention Through the Triangle Project

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

*Keywords:* Adolescent; Alcohol Drinking; Portugal. from #Medicine via ola Kala on Inoreader http://ift.tt/1HicEhl via IFTTT

Haemorrhagic Gingival Hypertrophy: A Striking Presentation of Acute Myelomonocytic Leukaemia

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

*Keywords:* Gingival Hypertrophy; Leukemia, Myelomonocytic, Acute. from #Medicine via ola Kala on Inoreader http://ift.tt/1Q12GrF via IFTTT

Pantothenate Kinase Associated Neurodegeneration in Two Brothers

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

*Keywords:* Neurodegenerative Diseases; Phosphotransferases (Alcohol Group Acceptor); Siblings. from #Medicine via ola Kala on Inoreader http://ift.tt/1HicGFS via IFTTT

Takotsubo Cardiomyopathy: Cause of a Cardiogenic Shock

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

Takotsubo cardiomyopathy, of unknown etiology, is characterized by sudden and transient systolic dysfunction of the mid-apical segments of the left ventricle without significant coronary disease, and full normalization of segmental changes. More common in middle-aged women, it is cause of differential diagnosis with acute coronary syndrome. We present the case of a 59 year old woman admitted to the emergency room with sudden chest pain and dyspnea. At presentation: acute hypotensive pulmonary edema requiring aminergic support and invasive ventilation. Blood tests showed elevated n...περισσότερα »

Auto-Immune Pancreatitis: The Diagnostic Role of Imaging Studies

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

Auto-immune pancreatitis is an uncommon and underdiagnosed pathology. Its clinical picture is not specific and the diagnosis is therefore difficult. Imaging studies have a crucial role in the diagnostic process and also in the follow-up of treatment. With the objective of emphasizing the importance of imaging in the correct diagnosis of this pathology, the authors report the case of a patient with longterm unspecific abdominal complaints that was diagnosed as auto-immune pancreatitis after laboratorial and imaging investigation. Assuming this diagnosis it was instituted treatment ... περισσότερα »

Hypothyroidism Associated to TSH Hormone-Receptor Autoantibodies with Blocking Activity Assessed In Vitro

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

Thyroid-stimulating hormone-receptor autoantibodies normally causes hyperthyroidism. However, they might have blocking activity causing hypothyroidism. A 11-year-old girl followed due to type 1 diabetes mellitus, celiac disease and euthyroid lymphocytic thyroiditis at diagnosis. Two years after the initial evaluation, thyroid-stimulating hormone was suppressed with normal free T4; nine months later, a biochemical evolution to hypothyroidism with thyroid-stimulating hormone-receptor autoantibodies elevation was seen; the patient remained always asymptomatic. Chinese hamster ovary c... περισσότερα »

Periarticular Corticosteroid Injection in the Therapeutic Approach of Musculoskeletal Disease in General Practice: A systematic Review

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

*Introduction:* Periarticular corticosteroid injection utilization by General Practitioners treating musculoskeletal disease in primary health care system is internationally acknowledged. This article aims at analyzing this evidence and contributing to the discussion of a potential development of the technique in Portugal. *Material and Methods:* Qualitative analysis of randomized control trials, cost-effectiveness studies, cross-sectional studies, retrospective cohort studies and observational studies, using PRISMA model. Data Sources: PubMed, Cochrane Library and Essential Evidence... περισσότερα »

Monoclonal Antibodies for Multiple Sclerosis Treatment

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

Since their introduction in medical therapy, in the last quarter of the 20th century, monoclonal antibodies have gained an increasing importance in the treatment of various diseases. Neurology has been one of the medical specialties benefiting of the therapeutic potential of these monoclonal antibodies and certain neurological conditions may now contain such drugs in their therapeutic algorithms. Multiple sclerosis is one of these diseases and, in addition to the monoclonal antibodies already licensed for clinical use, several others are in development for future utilization in th... περισσότερα »

Current Clinical Indications for Small Bowel Capsule Endoscopy

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

*Introduction:* Small bowel capsule endoscopy is currently the first line diagnostic examination for many diseases affecting the small bowel. This article aims to review and critically address the current indications of small bowel capsule endoscopy in clinical practice. *Material and Methods:* Bibliographic review of relevant and recent papers indexed in PubMed. *Results and Discussion:* Small bowel capsule endoscopy enables a non-invasive full-assessment of the small bowel mucosa, with high diagnostic yield even for subtle lesions. In patients with obscure gastrointestinal bleedi... περισσότερα »

Mediterranean Spotted Fever: Retrospective Review of Hospitalized Cases and Predictive Factors of Severe Disease

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

*Introduction:* Mediterranean spotted fever, an ancient zoonotic disease raising current issues. This study aims to reevaluate the disease clinical picture and to identify prognostic factors related to severe disease. *Material and Methods:* We evaluate cases admitted to a central hospital during 12-year period. The risk factors were determined by uni and multivariate analysis, comparing patients admitted in general infirmary versus intermediate/intensive care units or in case of death. *Results:* We reviewed 71 cases of Mediterranean spotted fever. The mean age was 63.3 ± 16.7 years ...περισσότερα »

Acute Ischemic Stroke on Cancer Patients, a Distinct Etiology? A Case-Control Study

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

*Introduction:* It is still unclear whether the etiology of ischemic stroke differs between cancer and non-cancer patients. Stroke and cancer share common modifiable risk factors but evidence suggests that cancer patients have specific conditions that increase the risk of stroke. Our goal was to compare the etiology of ischemic stroke in cancer and non-cancer patients. *Material and Methods:* Case-control study conducted in patients admitted to a stroke unit between January 2007 and December 2012. Cases had a concomitant diagnosis of cancer and acute ischemic stroke, controls of onl... περισσότερα »

Suicidal Behavior among Portuguese Psychiatry Trainees: Comparison with the European Situation

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

*Introduction:* The aim of this paper was to assess the prevalence of suicide ideation and attempts in Portuguese psychiatry trainees (adult and child and adolescence), and compare the data with the general population and other European countries. *Material and Methods:* A structured and anonymous questionnaire was sent by email to 159 portuguese trainees of adult psychiatry and child and adolescence psychiatry with questions about personal history of suicidal ideation and suicide attempts, as well as family history of suicide attempts and completed suicides. This is part of the BoSS... περισσότερα »

Neonatal Morbidity in Term Newborns Born by Elective Cesarean Section

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

*Introduction:* International guidelines suggest that non-urgent planned deliveries be scheduled at or after 39 weeks. Despite this recommendation elective cesarean often occurs before 39 weeks. Some research has demonstrated that elective cesarean before 39 weeks poses a greater risk to the infants than at or after 39 weeks. *Objective:* To evaluate neonatal morbidity in term newborns born by elective cesarean section. *Material and Methods:* Retrospective study of all term elective cesarean sections (scheduled and without labor) performed in level III maternity, in the last 11 year... περισσότερα »

Translation, Cultural Adaptation and Validation of the Power of Food Scale for Use by Adult Populations in Portugal

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

*Introduction:* The Power of Food Scale measures appetite for, rather than consumption of, palatable foods - a construct frequently designated as hedonic hunger. The purpose of this study was to examine the psychometric properties of a Portuguese version of the Power of Food Scale, namely reliability and construct validity. *Material and Methods:* Data was obtained from 1266 adult participants sampled from three different populations in Portugal: two nonclinical samples and one multi-center sample of severely obese candidates for weight-loss surgery. *Results:* We found that the fa... περισσότερα »

Quality of Recovery after Anesthesia: Validation of the Portuguese Version of the “Quality of Recovery 15” Questionnaire

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

*Background:* The "Quality of Recovery 15" questionnaire is used for the study of quality recovery after anesthesia. The aim of this study was to validate the Portuguese version of "Quality of Recovery 15" questionnaire. *Material and Methods:* After study approval by the institutional ethics committee, an observational and cohort prospective study was conducted on patients scheduled for elective surgery from June to August 2013. The "Quality of Recovery 15" questionnaire was translated in accordance with available guidelines. The "Quality of Recovery 15" Portuguese version was use... περισσότερα »

The Importance of Early Referral in Pediatric Acute Liver Failure

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

*Introduction:* Acute liver failure is a rare disorder associated to high morbidity and mortality despite survival improvement through liver transplantation. The importance of a multidisciplinary approach and early referral to a pediatric liver transplantation center were important conclusions of a national meeting in 2008, from which resulted an actuation consensus. *Objectives:* To characterize acute liver failure admissions in a Pediatric Intensive Care Unit of the portuguese pediatric liver transplantation center. To compare results before (A) and after (B) 2008. *Material and Met... περισσότερα »

Characterization and Evolution of Avoidable Admissions in Portugal: The Impact of Two Methodologic Approaches

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 3 ώρες

*Introduction:* The aim of this study is to evaluate the health systems performance through the avoidable hospital admissions, once these have gained international relevance. We used two different methods to identify the admissions for Ambulatory Care Sensitive Conditions, describing the Portuguese reality and evolution. *Material and Methods:* Over 12 million hospitalizations were analyzed between 2000 and 2012 using the national hospital discharge databases. We used two different methodologies to identify the hospitalizations for Ambulatory Care Sensitive Conditions, determining th...περισσότερα »

Thirdhand Smoke: A Concept to Remember!

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 4 ώρες

*Keywords:* Tobacco Smoke Pollution; Smoking. from #Medicine via ola Kala on Inoreader http://ift.tt/1HicwOF via IFTTT

Prophylactic use of the Arabin cervical pessary in fetuses with severe congenital diaphragmatic hernia treated by fetoscopic endoluminal tracheal occlusion (FETO): preliminary experience

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 5 ώρες

Abstract Objective To describe whether the prophylactic use of a cervical pessary decreases the rate of premature birth in congenital diaphragmatic hernia (CDH) fetuses treated with fetoscopic tracheal occlusion (FETO). Methods Consecutive series of all cases with CDH and FETO and a group of CDH without FETO. In a subgroup of the FETO group, a prophylactic cervical pessary was inserted the day following the procedure. Gestational age (GA) at birth was the primary outcome. Results 59 fetuses with FETO and 47 expectantly managed were included. The last 15 FETO had a cervical pess...περισσότερα »

Medial temporal lobe atrophy, white matter hyperintensities and cognitive impairment among Nigerian African stroke survivors

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 5 ώρες

Background: Neuroimaging features associated with vascular cognitive impairment have not been examined in sub-Saharan Africans. We determined magnetic resonance imaging (MRI) features associated with cognitive impairment in a sample of Nigerian stroke survivors. Methods: Stroke survivors underwent brain MRI with standardized assessment of brain volumes and visual rating of medial temporal lobe atrophy (MTA), and white matter hyperintensities (WMH) at 3 months post-stroke. Demographic, clinical and psychometric assessments of global cognitive function, executive function, mental sp...περισσότερα »

Pulsed Direct Current Electrospray: Enabling Systematic Analysis of Small Volume Sample by Boosting Sample Economy

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 6 ώρες

[image: TOC Graphic] Analytical Chemistry DOI: 10.1021/acs.analchem.5b02115 [image: ancham?d=yIl2AUoC8zA] from #Medicine via ola Kala on Inoreader http://ift.tt/1XG3pAX via IFTTT

Sensitive Trimodal Magnetic Resonance Imaging-Surface-Enhanced Resonance Raman Scattering-Fluorescence Detection of Cancer Cells with Stable Magneto-Plasmonic Nanoprobes

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 6 ώρες

[image: TOC Graphic] Analytical Chemistry DOI: 10.1021/acs.analchem.5b02419 [image: ancham?d=yIl2AUoC8zA] from #Medicine via ola Kala on Inoreader http://ift.tt/1XG3m8l via IFTTT

Enhancement of the Carbon Dots/K2S2O8 Chemiluminescence System Induced by Triethylamine

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 6 ώρες

[image: TOC Graphic] Analytical Chemistry DOI: 10.1021/acs.analchem.5b02562 [image: ancham?d=yIl2AUoC8zA] from #Medicine via ola Kala on Inoreader http://ift.tt/1XG3lRz via IFTTT

CIUSuite: A Quantitative Analysis Package for Collision Induced Unfolding Measurements of Gas-Phase Protein Ions

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 6 ώρες

[image: TOC Graphic] Analytical Chemistry DOI: 10.1021/acs.analchem.5b03292 [image: ancham?d=yIl2AUoC8zA] from #Medicine via ola Kala on Inoreader http://ift.tt/1XG3k06 via IFTTT

Imaging of Proteins in Tissue Samples Using Nanospray Desorption Electrospray Ionization Mass Spectrometry

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 8 ώρες

[image: TOC Graphic] Analytical Chemistry DOI: 10.1021/acs.analchem.5b03389 [image: ancham?d=yIl2AUoC8zA] from #Medicine via ola Kala on Inoreader http://ift.tt/1PaIPWd via IFTTT

Modified-Atmospheric Pressure-Matrix Assisted Laser Desorption/Ionization Identification of Friction Modifier Additives Oleamide and Ethoxylated Tallow Amines on Varied Metal Target Materials and Tribologically Stressed Steel Surfaces

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 8 ώρες

[image: TOC Graphic] Analytical Chemistry DOI: 10.1021/acs.analchem.5b02793 [image: ancham?d=yIl2AUoC8zA] from #Medicine via ola Kala on Inoreader http://ift.tt/1PaIP8W via IFTTT

To Your Health: NLM update: Explaining health risks in drug advertising

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 9 ώρες

[image: Listen to the NLM Director's Comments] Listen to the To Your Health: NLM update on Explaining health risks in drug advertising. The transcript is also available. The risks of U.S. Food and Drug Administration (FDA)-approved medications should be explained with the same dexterity as promoting its benefits by direct-to-consumer advertisers, suggests an interesting perspective recently published in The New England Journal of Medicine. from #Medicine via ola Kala on Inoreader http://ift.tt/1OegKyJ via IFTTT

To Your Health: NLM update: Ebola vaccine's initial success

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 9 ώρες

[image: Listen to the NLM Director's Comments] Listen to the To Your Health: NLM update on Ebola vaccine's initial success. The transcript is also available. A vaccine to prevent Ebola was successful in an initial clinical trial within the West African nation of Guinea, reports a pioneering study and editorial recently published in The Lancet. from #Medicine via ola Kala on Inoreader http://ift.tt/1GCixuV via IFTTT

Tomosynthesis Imaging

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 12 ώρες

This article reviews Tomosynthesis Imaging Imaging in Medical Diagnosis and Therapy. Editors: I. Reiser and S. Glick. Series Editor: William R. Hendee, , Boca Raton, FL, 2014. 257 pp. Price: $144.46. ISBN: 978143987870 (hardback). from #Medicine via ola Kala on Inoreader http://ift.tt/1kgWLlY via IFTTT

Erratum: “Analytic variance estimates of Swank and Fano factors” [Med. Phys. 41, 072102 (5pp.) (2014)]

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 12 ώρες

from #Medicine via ola Kala on Inoreader http://ift.tt/1OdVU2C via IFTTT

Erratum: “Modeling respiratory motion for reducing motion artifacts in 4D CT images” [Med. Phys. 40, 041716 (13pp.) (2013)]

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 12 ώρες

from #Medicine via ola Kala on Inoreader http://ift.tt/1kgWL5A via IFTTT

Optimization of an on-board imaging system for extremely rapid radiation therapy

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 12 ώρες

Purpose: Next-generation extremely rapid radiation therapy systems could mitigate the need for motion management, improve patient comfort during the treatment, and increase patient throughput for cost effectiveness. Such systems require an on-board imaging system that is competitively priced, fast, and of sufficiently high quality to allow good registration between the image taken on the day of treatment and the image taken the day of treatment planning. In this study, three different detectors for a custom on-board CT system were investigated to select the best design for integr... περισσότερα »

Image-derived and arterial blood sampled input functions for quantitative PET imaging of the angiotensin II subtype 1 receptor in the kidney

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 12 ώρες

Purpose: The radioligand 11C-KR31173 has been introduced for positron emission tomography (PET) imaging of the angiotensin II subtype 1 receptor in the kidney in vivo. To study the biokinetics of 11C-KR31173 with a compartmental model, the input function is needed. Collection and analysis of arterial blood samples are the established approach to obtain the input function but they are not feasible in patients with renal diseases. The goal of this study was to develop a quantitative technique that can provide an accurate image-derived input function (ID-IF) to replace the conventio...περισσότερα »

A round-robin gamma stereotactic radiosurgery dosimetry interinstitution comparison of calibration protocols

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 12 ώρες

Purpose: Absorbed dose calibration for gamma stereotactic radiosurgery is challenging due to the unique geometric conditions, dosimetry characteristics, and nonstandard field size of these devices. Members of the American Association of Physicists in Medicine (AAPM) Task Group 178 on Gamma Stereotactic Radiosurgery Dosimetry and Quality Assurance have participated in a round-robin exchange of calibrated measurement instrumentation and phantoms exploring two approved and two proposed calibration protocols or formalisms on ten gamma radiosurgery units. The objectives of this study ... περισσότερα »

A simple clot based assay for detection of procoagulant cell-derived microparticles

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 12 ώρες

Journal Name: Clinical Chemistry and Laboratory Medicine (CCLM) Issue: Ahead of print from #Medicine via ola Kala on Inoreader http://ift.tt/1NepYcU via IFTTT

Establishment of reference intervals of clinical chemistry analytes for adult population in Saudi Arabia: a study conducted as a part of the IFCC global study on reference values

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 12 ώρες

Journal Name: Clinical Chemistry and Laboratory Medicine (CCLM) Issue: Ahead of print from #Medicine via ola Kala on Inoreader http://ift.tt/1NepVxU via IFTTT

Incidence and determinants of neonatal morbidity after elective caesarean section at the national referral hospital in Kampala, Uganda

Αλέξανδρος ΣφακιανάκηςστοMedicine by Alexandros G.SfakianakisΠριν από 13 ώρες

Background: Elective caesarean sections (ECS) have been implicated in increased risk of adverse neonatal outcomes. The primary objective was to assess the incidence and determinants of neonatal morbidity after elective caesarean section deliveries. The secondary objective was to describe the maternal morbidity associated with elective caesarean section. Methods: This was a prospective cohort study of women admitted for ECS, as well as their newborns, conducted at Mulago hospital from March 1, 2013 to February 28, 2014. These were followed from the time of the operation until 6 week...περισσότερα »

Proton pump inhibitor and voice therapy on reflux-related laryngeal disorders

Alexandros G. SfakianakisστοOtoRhinoLaryngology - Alexandros G. SfakianakisΠριν από 17 ώρες

*ORIGINAL ARTICLEYear *: 2015 | *Volume* : 43 | *Issue* : 4 | *Page* : 127--133 Effect of proton pump inhibitor and voice therapy on reflux-related laryngeal disorders *Hatem Ezzeldin1, Ahmed A Hasseba2* 1 Department of Otorhinolaryngology, Sohag University, Sohag, Egypt; Department of Phoniatrics, King Fahd Hospital Jeddah, Jeddah, Kingdom of Saudi Arabia, 2 Department of Otorhinolaryngology, El-Minia University, Minya, Egypt *Correspondence Address*: Hatem Ezzeldin (Phoniatrics), Department of Phoniatrics, King Fahd Hospital Jeddah, PO Box 8488, Jeddah 21196 *Objectives* The...περισσότερα »