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- Minimally Invasive Versus Open Pancreaticoduodenec...
- The Southampton Consensus Guidelines for Laparosco...
- Dynamic Angiogenic Switch as Predictor of Response...
- Hereditary ataxias and paraparesias: clinical and ...
- Cerebrovascular disorders
- The changing landscape of motor neuron disease ima...
- Update on amino acid PET of brain tumours
- Dystonia as a network disorder: a concept in evolu...
- Neuroimaging of brain trauma
- Progressive supranuclear palsy and multiple system...
- Neuroimaging in epilepsy
- Myoclonus-dystonia: classification, phenomenology,...
- Recent advances in headache neuroimaging
- Editorial introductions
- MRI in multiple sclerosis: what is changing?
- Update in therapeutic strategies for Parkinson's d...
- Alzheimer's disease neuroimaging
- The patchy tremor landscape: recent advances in pa...
- MRI of non-Alzheimer's dementia: current and emerg...
- Movement disorders in mitochondrial disease: a cli...
- Neuroimaging advances in Parkinson's disease
- The expanding spectrum of paroxysmal movement diso...
- Molecular mechanisms of acquired resistance to thi...
- Phase II study of nab-paclitaxel in refractory sma...
- Global patterns of care in advanced stage mycosis ...
- Contender waveforms for Low-Power Wide-Area networ...
- Potentiation of PD-L1 blockade with a potency-matc...
- Systemic-inflammation-based score can predict prog...
- Effects of physicochemical parameters on volatile ...
- Poly-l-gamma-glutamic acid production by recombina...
- Investigation into the potential for post-mortem f...
- Optimising Genomic Selection in Wheat: Effect of M...
- AMA Urges Caution With Use of Wire-Bristle BBQ Gri...
- FDA Permits Marketing of Devices to Create Arterio...
- HPV Cervical CA Screening Cuts Odds of Later CIN3+...
- Vegetable Trays Tainted With Cyclospora Put Seven ...
- Oral Insulin Choline, Geranate Ionic Liquid Shows ...
- Utility of Repeat Magnetic Resonance Imaging in Su...
- Consensus on Molecular Subtypes of High-grade Sero...
- Final Report of a Phase I Trial of Olaparib with C...
- Parallel Accumulation of Tumor Hyaluronan, Collage...
- Combined CDK4/6 and pan-mTOR inhibition is synergi...
- Mast cell activation and KSHV infection in Kaposi ...
- The epigenomic landscape of pituitary adenomas rev...
- Paclitaxel Sensitivity of Ovarian Cancer can be en...
- Comparison of longitudinal CA125 algorithms as a f...
- The Importance of the Double Product in the Six-Mi...
- The Social Media Index as an Indicator of Quality ...
- Emergency Department Urinary Tract Infections Caus...
- Point-of-Care Ultrasound Assessment of Bladder Ful...
- Selection of Intravenous Fluids
- Child and Parental Perspectives on Communication a...
- A foodborne acute gastroenteritis outbreak caused ...
- Coping strategies among nurses in South-west Ethio...
- Student performance on the Test of Scientific Lite...
- Sero-prevalence of hepatitis B virus infection and...
- Modes of mechanical ventilation vary between hospi...
- T-Cell Receptor Excision Circles in HIV-Exposed, U...
- Association Between Video Laryngoscopy and Adverse...
- Predictor of multidrug resistant tuberculosis in s...
- Gliadin effect on the oxidative balance and DNA da...
- Neuropilin-1 (NRP-1) upregulated by IL-6/STAT3 sig...
- Conflicting IDH1/IDH2, ATRX, and 1p/19q results in...
- Elevated DSN1 expression is associated with poor s...
- Atypical mitoses are present in otherwise classica...
- Clonality assessment of multifocal lung adenocarci...
- Atypical mitoses are present in otherwise classica...
- IgA dominant glomerulonephritis with a membranopro...
- Exploration of BRAFV600E as a diagnostic adjuvant ...
- Eps8, a therapeutic potential target for cancer
- Targeted gene sequencing of lynch syndrome-related...
- Hepatocellular Carcinoma Invading the Gastric Antrum
- Effect of BCLAF1 on HDAC inhibitor LMK-235-mediate...
- Association between Mismatch Negativity and Voxel-...
- Reply to “Clinical Practice Guidelines or Clinical...
- Clinical Practice Guidelines or Clinical Research ...
- Successful liver-directed gene delivery by ERCP-gu...
- Impact of whole lung irradiation on survival outco...
- High Blood Pressure During Pregnancy and Developme...
- Hypertensive Disorders of Pregnancy and Maternal C...
- Women's Cardiovascular Health After a Hypertensive...
- Recurrent Renal Cysts in a Transplanted Kidney
- Marijuana Use, Respiratory Symptoms, and Pulmonary...
- Chemical composition, antioxidant, antimicrobial a...
- 10-hydroxy-2-decenoic acid of royal jelly exhibits...
- Reducing antibiotic use for uncomplicated urinary ...
- Cytotoxic properties of the anthraquinone derivati...
- Antibacterial interactions, anti-inflammatory and ...
- Associations Between Gun Shows and Firearm Deaths ...
- Implications of Nine Risk Prediction Models for Se...
- Annals for Educators - 3 July 2018
- Clinical Implications of Revised Pooled Cohort Equ...
- Comparison of Five Major Guidelines for Statin Use...
- ESPACOMP Medication Adherence Reporting Guideline ...
- Bronchial Thermoplasty for Severe Asthmatic Cough
- Machine Learning and Evidence-Based Medicine
- Comparison of Five Major Guidelines for Statin Use...
- Firearms and Dementia: Clinical Considerations
- Emergency Room
- Physician Burnout in the Electronic Health Record ...
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Αναζήτηση αυτού του ιστολογίου
Τρίτη 3 Ιουλίου 2018
Minimally Invasive Versus Open Pancreaticoduodenectomy: A Propensity-matched Study From a National Cohort of Patients
https://ift.tt/2l4YWvB
The Southampton Consensus Guidelines for Laparoscopic Liver Surgery: From Indication to Implementation
https://ift.tt/2t6s6ym
Dynamic Angiogenic Switch as Predictor of Response to Chemotherapy-Bevacizumab in Patients With Metastatic Colorectal Cancer
https://ift.tt/2u0MPnz
Hereditary ataxias and paraparesias: clinical and genetic update
https://ift.tt/2KtxBlX
Cerebrovascular disorders
https://ift.tt/2KJrwxL
The changing landscape of motor neuron disease imaging: the transition from descriptive studies to precision clinical tools
https://ift.tt/2KtxqXP
Update on amino acid PET of brain tumours
https://ift.tt/2KNNnEm
Dystonia as a network disorder: a concept in evolution
https://ift.tt/2Ktxdnv
Neuroimaging of brain trauma
https://ift.tt/2z8LlNJ
Progressive supranuclear palsy and multiple system atrophy: clinicopathological concepts and therapeutic challenges
https://ift.tt/2Ktx2Zn
Neuroimaging in epilepsy
https://ift.tt/2KJr56B
Myoclonus-dystonia: classification, phenomenology, pathogenesis, and treatment
https://ift.tt/2KzPFLy
Recent advances in headache neuroimaging
https://ift.tt/2IOKG3K
MRI in multiple sclerosis: what is changing?
https://ift.tt/2IVnYXA
Update in therapeutic strategies for Parkinson's disease
https://ift.tt/2KzPrnG
Alzheimer's disease neuroimaging
https://ift.tt/2IQHy7o
The patchy tremor landscape: recent advances in pathophysiology
https://ift.tt/2KzPjoc
MRI of non-Alzheimer's dementia: current and emerging knowledge
https://ift.tt/2IS8Nyt
Movement disorders in mitochondrial disease: a clinicopathological correlation
https://ift.tt/2KIsde8
Neuroimaging advances in Parkinson's disease
https://ift.tt/2Nq71rJ
The expanding spectrum of paroxysmal movement disorders: update from clinical features to therapeutics
https://ift.tt/2z9Jp7Q
Contender waveforms for Low-Power Wide-Area networks in a scheduled 4G OFDM framework
When designing new solutions for Low-Power Wide-Area (LPWA) networks, coexistence and integration into existing 4G frameworks should be considered to ease the deployment procedure and reduce costs. In a previo...
https://ift.tt/2lS0For
Potentiation of PD-L1 blockade with a potency-matched dual cytokine–antibody fusion protein leads to cancer eradication in BALB/c-derived tumors but not in other mouse strains
Abstract
We have recently described a novel therapeutic antibody product (IL2–F8–TNFmut), featuring the simultaneous fusion of murine IL2 and of a TNF mutant with scFv(F8), an antibody specific to the alternatively-spliced extra domain A of fibronectin (EDA). Here, we report on the in vivo characterization of the anti-cancer activity of IL2–F8–TNFmut in four immunocompetent murine models of cancer, CT26, WEHI-164, F9 teratocarcinoma and Lewis lung carcinoma (LLC), using the product alone or in combination with a monoclonal antibody specific to murine PD-L1. All four models exhibited a strong expression of EDA-fibronectin, which was confined to vascular structures for F9 tumors, while the other three malignancies exhibited a more stromal pattern of staining. A complete and long-lasting tumor eradication of CT26 and WEHI-164 tumors was observed in BALB/c mice when IL2–F8–TNFmut was used in combination with PD-L1 blockade. The combination treatment led to improved tumor growth inhibition in 129/SvEv mice bearing murine teratocarcinoma or in C57BL/6 mice bearing murine LLC, but those cancer cures were difficult to achieve in those models. A microscopic analysis of tumor sections, obtained 24 h after pharmacological treatment, revealed that the PD-L1 antibody had homogenously reached tumor cells in vivo and that the combination of PD-L1 blockade with IL2–F8–TNFmut stimulated an influx of NK cells and of T cells into the neoplastic mass. These data indicate that potency-matched dual-cytokine fusion proteins may be ideally suited to potentiate the therapeutic activity of immune check-point inhibitors.
https://ift.tt/2Kylt39
Systemic-inflammation-based score can predict prognosis in metastatic gastric cancer patients before first-line chemotherapy
Future Oncology, Ahead of Print.
https://ift.tt/2KIrVE0
Effects of physicochemical parameters on volatile sulphur compound formation from l-methionine catabolism by non-growing cells of Kluyveromyces lactis
The present study investigated for the first time the effects of various physicochemical parameters on the production of volatile sulphur compounds (VSCs) by non-growing cells of Kluyveromyces lactis supplemented...
https://ift.tt/2u3ku01
Poly-l-gamma-glutamic acid production by recombinant Bacillus subtilis without pgsA gene
Poly-gamma-glutamic acid (PGA) is a promising bio-based polymer that shares many functions with poly (acrylic acid) and its derivatives. Thus, technologies for efficient production and molecular size control o...
https://ift.tt/2lRTbBR
Investigation into the potential for post-mortem formation of carboxyhemoglobin in bodies retrieved from fires
Abstract
The forensic investigation of a deceased person retrieved following a fire includes measuring carboxyhemoglobin. A carboxyhemoglobin saturation above 10% is considered indicative of respiration during a fire, implying the person had been alive. This relies on the assumption that carbon monoxide will not diffuse into blood used for toxicological analysis. This project investigated the potential for carbon monoxide to passively diffuse into a body and if carboxyhemoglobin levels could become elevated post-mortem. Stillborn piglets with intact skin were exposed to carbon monoxide. Carboxyhemoglobin formed in the hypostasis of the skin, but carboxyhemoglobin levels in blood from the heart and chest cavities were not significantly elevated. However, defects in the skin over body cavities (producing breaches to replicate cases with stab wounds or heat damage) resulted in cavity blood carboxyhemoglobin levels above 10%. A review of fire death cases in South Australia 2000–2015 was performed to determine the origin of the blood samples used for toxicological analysis and the incidence of cases with breaches of body cavities. This revealed a small number of cases in which blood from the cavities had been analyzed when cavity breaches were present. Thus, there is a potential for significant elevation of carboxyhemoglobin saturation post-mortem in forensic casework involving bodies retrieved from fires.
https://ift.tt/2KxoMb1
Optimising Genomic Selection in Wheat: Effect of Marker Density, Population Size and Population Structure on Prediction Accuracy
Genomic selection applied to plant breeding enables earlier estimates of a line's performance and significant reductions in generation interval. Several factors affecting prediction accuracy should be well understood if breeders are to harness genomic selection to its full potential. We used a panel of 10,375 bread wheat (Triticum aestivum) lines genotyped with 18,101 SNP markers to investigate the effect and interaction of training set size, population structure and marker density on genomic prediction accuracy. Through assessing the effect of training set size we showed the rate at which prediction accuracy increases is slower beyond approximately 2,000 lines. The structure of the panel was assessed via principal component analysis and K-means clustering, and its effect on prediction accuracy was examined through a novel cross-validation analysis according to the K-means clusters and breeding cohorts. Here we showed that accuracy can be improved by increasing the diversity within the training set, particularly when relatedness between training and validation sets is low. The breeding cohort analysis revealed that traits with higher selection pressure (lower allelic diversity) can be more accurately predicted by including several previous cohorts in the training set. The effect of marker density and its interaction with population structure was assessed for marker subsets containing between 100 and 17,181 markers. This analysis showed that response to increased marker density is largest when using a diverse training set to predict between poorly related material. These findings represent a significant resource for plant breeders and contribute to the collective knowledge on the optimal structure of calibration panels for genomic prediction.
https://ift.tt/2tVEX7u
AMA Urges Caution With Use of Wire-Bristle BBQ Grill Brushes
TUESDAY, July 3, 2018 -- The American Medical Association (AMA) states that caution should be exercised with use of wire-bristle grill brushes due to the potential health and safety risks associated with bristles that may break off and adhere to the...
https://ift.tt/2KKjBUn
FDA Permits Marketing of Devices to Create Arteriovenous Fistula
TUESDAY, July 3, 2018 -- The U.S. Food and Drug Administration has permitted marketing of two catheter-based devices designed to create an arteriovenous (AV) fistula in patients with chronic kidney disease in need of hemodialysis. The devices are...
https://ift.tt/2KHUYnY
HPV Cervical CA Screening Cuts Odds of Later CIN3+ Diagnosis
TUESDAY, July 3, 2018 -- The use of primary human papillomavirus (HPV) testing versus cytology results in reduced likelihood of cervical intraepithelial neoplasia (CIN) grade 3 or worse (CIN3+) at 48 months, according to a study published in the...
https://ift.tt/2KysIIF
Vegetable Trays Tainted With Cyclospora Put Seven in Hospital
TUESDAY, July 3, 2018 -- Federal, state, and local health officials are investigating a Cyclospora outbreak linked to Del Monte 6 oz and 12 oz vegetable trays containing broccoli, cauliflower, carrots, and dill dip that were sold at Kwik Trip/Kwik...
https://ift.tt/2INAFDX
Oral Insulin Choline, Geranate Ionic Liquid Shows Promise
TUESDAY, July 3, 2018 -- An oral insulin formulation has been developed using choline and geranate (CAGE) ionic liquid, which significantly reduces blood glucose levels in vivo for a sustained period, according to a study published online June 25 in...
https://ift.tt/2KGn4mS
Utility of Repeat Magnetic Resonance Imaging in Surgical Patients with Lumbar Stenosis without Disc Herniation
Publication date: Available online 3 July 2018
Source:The Spine Journal
Author(s): Bryan S. Lee, Rod Nault, Matthew Grabowski, Benjamin Whiting, Joseph Tanenbaum, Konrad Knusel, Matthew Poturalski, Todd Emch, Thomas E. Mroz, Michael P. Steinmetz
BACKGROUND CONTEXTRoutine use of magnetic resonance imaging (MRI) as a diagnostic tool in lumbar stenosis is becoming more prevalent due to the aging population. Currently there is no clinical guideline to clarify the utility of repeat MRI in patients with lumbar stenosis, without instability, neurological deficits, or disc herniation.PURPOSETo evaluate the utility of routine use of MRI as a diagnostic tool in lumbar stenosis, and to help formulate clinical guidelines on the appropriate use of preoperative imaging for lumbar stenosisSTUDY DESIGN/SETTINGRetrospective radiographic analysisPATIENT SAMPLERetrospective chart review was performed to review patients with lumbar stenosis, who underwent lumbar decompression without fusion from 2011 to 2015 at a single institution.OUTCOME MEASURESPreviously established stenosis grading systems were used to measure and compare the initial and the subsequent repeat lumbar MRI's performed preoperatively. If patients were found to have a moderate or severe grade change, and if the surgical plan was altered due to such exacerbated radiographic findings, then their grade changes were considered clinically meaningful.METHODSWe identified patients with lumbar stenosis without radiographic instability or neurological deficits, who had at least two preoperative lumbar MRI's performed and underwent decompressive surgeries. At each pathologic disc level, the absolute value of the change in grade for central/lateral recess stenosis, right foraminal stenosis, and left foraminal stenosis from the first preoperative MRI to the repeated MRI was calculated. These change data were then used to calculate the mean and median changes in each of the three types of stenosis for each pathologic disc level. Identical calculations were carried out for the subsample of patients who only underwent discectomy or had a discectomy included as part of their surgery.RESULTSAmong the 103 patients who met the inclusion criteria, 37 of those patients had more than one level surgically addressed, and a total of 161 lumbar levels were reviewed. Among the subset of patients that had any grade change, the majority of the grades only had a mild change of 1 (36 out of 42 patients, 85.7%, 95% confidence interval [CI]: 73.1%-94.1%); there was a moderate grade change of 2 in two patients (4.8%, CI: 0.8%-14.0%), and a severe change of 3 in one patient (2.4%, CI: 0.2%-10.1%). There were three patients with decreased grade change (7.1%, CI: 1.8%-17.5%). All clinically meaningful grade changes were from the subset of patients who had only discectomy or discectomy as part of the procedure. Lastly, both patients that had a clinically meaningful grade change had their MRI's performed at an interval of greater than 360 days.CONCLUSIONSThe radiographic evaluation of the utility of routinely repeated MRI's in lumbar stenosis without instability, neurological deficits, or disc herniations demonstrated that there were no significant changes found in the repeated MRI in the preoperative setting, especially if the MRI's were performed less than one year apart. The results of the present study can help to standardize the diagnostic evaluation of lumbar stenosis and to formulate clinical guidelines on the appropriate use of preoperative imaging for lumbar stenosis patients.
https://ift.tt/2lTJX8s
Consensus on Molecular Subtypes of High-grade Serous Ovarian Carcinoma
Purpose: The majority of ovarian carcinomas are of high-grade serous histology, which is associated with poor prognosis. Surgery and chemotherapy are the mainstay of treatment, and molecular characterization is necessary to lead the way to targeted therapeutic options. To this end, various computational methods for gene expression-based subtyping of high-grade serous ovarian carcinoma (HGSOC) have been proposed, but their overlap and robustness remain unknown. Experimental Design: We assess three major subtype classifiers by meta-analysis of publicly available expression data, and assess statistical criteria of subtype robustness and classifier concordance. We develop a consensus classifier that represents the subtype classifications of tumors based on the consensus of multiple methods, and outputs a confidence score. Using our compendium of expression data, we examine the possibility that a subset of tumors are unclassifiable based on currently proposed subtypes. Results: HGSOC subtyping classifiers exhibit moderate pairwise concordance across our data compendium (58.9%-70.9%, p < 10-5) and are associated with overall survival in a meta-analysis across datasets (p < 10-5). Current subtypes do not meet statistical criteria for robustness to re-clustering across multiple datasets (Prediction Strength < 0.6). A new subtype classifier is trained on concordantly classified samples to yield a consensus classification of patient tumors that correlates with patient age, survival, tumor purity, and lymphocyte infiltration. Conclusion: A new consensus ovarian subtype classifier represents the consensus of methods, and demonstrates the importance of classification approaches for cancer that do not require all tumors to be assigned to a distinct subtype.
https://ift.tt/2MMUuNU
Final Report of a Phase I Trial of Olaparib with Cetuximab and Radiation for Heavy Smoker Patients with Locally Advanced Head and Neck Cancer.
Purpose: Our goal was to evaluate the safety and toxicity of combining a PARP inhibitor, olaparib with cetuximab and fractionated intensity modulated radiation therapy for patients with locally advanced head and neck cancer and heavy smoking histories. Experimental Design: Patients with ≥10 pack/year history of smoking were treated with olaparib at doses ranging from 25 mg-200 mg orally twice daily (BID) beginning ~10 days prior to initiation of and with concurrent radiation (69.3 Gy in 33 fractions) using a TITE-CRM model. Cetuximab was administered starting ~5 days prior to radiation per standard of care. Results: A total of 16 patients were entered onto the study with 15 evaluable for acute toxicity. The most common treatment-related Grade 3-4 side effects were radiation dermatitis and mucositis (38% and 69%, respectively). The maximum tolerated dose was determined to be 50 mg PO BID, but the RP2D was deemed to be 25 mg PO BID. At a median follow up of 26 months, the actuarial median overall survival was 37 months but was not reached for other endpoints. Two-year overall survival, progression free survival, local control, and distant control rates were 72%, 63%, 72%, 79%, respectively. Patients who continued to smoke during therapy experienced higher recurrence rates. MYCand KMT2Awere identified as potential correlatives of response on gene amplification and mutational analysis. Conclusion: Olaparib at 25 mg oral BID with concurrent cetuximab and radiation was well tolerated with reduced dermatitis within the radiation field. Responses rates were promising for this high-risk population.
https://ift.tt/2KrzGii
Parallel Accumulation of Tumor Hyaluronan, Collagen and Other Drivers of Tumor Progression
Purpose:The tumor microenvironment (TME) evolves to support tumor progression. One marker of more aggressive malignancy is hyaluronan (HA) accumulation. Here we characterize biological and physical changes associated with HA accumulating (HA-high) tumors. Experimental Design:We used immunohistochemistry, in vivo imaging of tumor pH, and microdialysis to characterize the TME of HA-high tumors, including tumor interstitial pressure (tIP), vascular structure, hypoxia, tumor perfusion by doxorubicin, pH, content of collagen and smooth muscle actin (α-SMA). A novel method was developed to measure real-time tumor-associated soluble cytokines and growth factors. We also evaluated biopsies of murine and pancreatic cancer patients to investigate HA and collagen content, important contributors to drug resistance. Results:In immunodeficient and immunocompetent mice, increasing tumor HA content is accompanied by increasing collagen content, tIP, vascular collapse, hypoxia, and increased metastatic potential, as reflected by increased α-SMA. In vivo treatment of HA-high tumors with PEGylated recombinant human hyaluronidase (PEGPH20) dramatically reversed these changes and depleted stores of VEGF-A165, suggesting that PEGPH20 may also diminish the angiogenic potential of the TME. Finally, we observed in xenografts and in pancreatic cancer patients a coordinated increase in HA and collagen tumor content. Conclusions: The accumulation of HA in tumors is associated with high tIP, vascular collapse, hypoxia, and drug resistance. These findings may partially explain why more aggressive malignancy is observed in the HA-high phenotype. We have shown that degradation of HA by PEGPH20 partially reverses this phenotype and leads to depletion of tumor-associated VEGF-A165. These results encourage further clinical investigation of PEGPH20.
https://ift.tt/2MOntkH
Combined CDK4/6 and pan-mTOR inhibition is synergistic against intrahepatic cholangiocarcinoma
Purpose: Intrahepatic cholangiocarcinoma (ICC) is an aggressive cancer type, lacking effective therapies and associated with a dismal prognosis. Palbociclib is a selective CDK4/6 inhibitor, which has been shown to suppress cell proliferation in many experimental cancer models. Recently, we demonstrated that pan-mTOR inhibitors, such as MLN0128, effectively induce apoptosis, while having limited efficacy in restraining proliferation of ICC cells. Here, we tested the hypothesis that Palbociclib, due to its ant-proliferative properties in many cancer types, might synergize with MLN0128 to impair ICC growth. Experimental Design: Human ICC cell lines and the AKT/YapS127A ICC mouse model were used to test the therapeutic efficacy of Palbociclib and MLN0128, either alone or in combination. Results: Administration of Palbociclib suppressed in vitro ICC cell growth by inhibiting cell cycle progression. Concomitant administration of Palbociclib and MLN0128 led to a pronounced, synergistic growth constraint of ICC cell lines. Furthermore, while treatment with Palbociclib or MLN0128 alone resulted in tumor growth reduction in AKT/YapS127A mice, a remarkable tumor regression was achieved when the two drugs were administered simultaneously. Mechanistically, Palbociclib was found to potentiate MLN0128 mTOR inhibition activity, whereas MLN0128 prevented the upregulation of cyclin D1 induced by Pa1bociclib treatment. Conclusions:Our study indicates the synergistic activity of Palbociclib and MLN0128 in inhibiting ICC cell proliferation. Thus, combination of CDK4/6 and mTOR inhibitors might represent a novel, promising, and effective therapeutic approach against human ICC.
https://ift.tt/2KGO5qk
Mast cell activation and KSHV infection in Kaposi sarcoma
Purpose: Kaposi sarcoma (KS) is a vascular tumor initiated by infection of endothelial cells (ECs) with KS-associated herpesvirus (KSHV). KS is dependent on sustained pro-inflammatory signals provided by intra-lesional leukocytes and continued infection of new ECs. However, the sources of these cytokines and infectious virus within lesions are not fully understood. Here, mast cells (MCs) are identified as pro-inflammatory cells within KS lesions that are permissive for, and activated by, infection with KSHV. Experimental Design: Three MC lines were used to assess permissivity to infection and to evaluate MC activation following infection. Biopsies from 31 AIDS-KS cases and 11 AIDS controls were evaluated by immunohistochemistry for the presence of MCs in KS lesions, assessment of activation state, and KSHV infection. Plasma samples from 26 AIDS-KS, 13 classic KS, and 13 healthy adults were evaluated for levels of MC granule contents tryptase and histamine. Results: In culture, MCs supported KSHV infection and infection induced MC degranulation. Within KS lesions MCs were closely associated with spindle cells. MC activation was extensive within KS patients, reflected by elevated circulating levels of tryptase and N-methylhistamine. One patient with clinical signs of extensive MC activation was treated with antagonists of MC-derived pro-inflammatory mediators, which resulted in rapid and durable regression of AIDS-KS lesions. Conclusions: Using complimentary in vitro and in vivo studies we identify MCs as a potential long-lived reservoir for KSHV and a source of pro-inflammatory mediators within the lesion microenvironment. Additionally, we identify MC antagonists as a promising novel therapeutic approach for KS.
https://ift.tt/2MJ5egq
The epigenomic landscape of pituitary adenomas reveals specific alterations and differentiates among acromegaly, Cushing's disease and endocrine-inactive subtypes
Purpose Pituitary adenomas (PAs) are one of the most common benign neoplasms of the central nervous system. Although emerging evidence suggests roles for both genetic and epigenetic factors in tumorigenesis, the degree to which these factors contribute to disease remains poorly understood. Experimental Design A multi-platform analysis was performed to identify the genomic and epigenomic underpinnings of disease among the three major subtypes of surgically-resected PAs in 48 patients: growth hormone (GH)-secreting (n=17), adrenocorticotropic hormone (ACTH)-secreting (n=13, including 3 silent-ACTH adenomas), and endocrine-inactive (n=18). Whole-exome sequencing was used to profile the somatic mutational landscape, whole-transcriptome sequencing was used to identify disease specific patterns of gene expression, and array-based DNA methylation profiling was used to examine genome-wide patterns of DNA methylation. Results Recurrent single nucleotide and small indel somatic mutations were infrequent among the three adenoma subtypes. However, somatic copy number alterations (SCNAs) were identified in all three PA subtypes. Methylation analysis revealed adenoma subtype-specific DNA methylation profiles, with GH-secreting adenomas being dominated by hypomethylated sites. Likewise, gene expression patterns revealed adenoma subtype-specific profiles. Integrating DNA methylation and gene expression data revealed that hypomethylation of promoter regions are related with increased expression of GH1 and SSTR5 genes in GH-secreting adenomas and POMCgene in ACTH-secreting adenomas. Finally, multispectral IHC staining of immune-related proteins showed abundant expression of PD-L1among all three adenoma subtypes. Conclusions Taken together, these data stress the contribution of epigenomic alterations to disease specific etiology among adenoma subtypes and highlight potential targets for future immunotherapy-based treatments.
https://ift.tt/2KKhuQi
Paclitaxel Sensitivity of Ovarian Cancer can be enhanced by knocking down Pairs of Kinases that regulate MAP4 Phosphorylation and Microtubule Stability
Purpose: Most ovarian cancer patients receive paclitaxel chemotherapy, but less than half respond. Pre-treatment microtubule stability correlates with paclitaxel response in ovarian cancer cell lines. Microtubule stability can be increased by depletion of individual kinases. As microtubule stability can be regulated by phosphorylation of microtubule associated proteins (MAPs), we reasoned that depletion of pairs of kinases that regulate phosphorylation of MAPs could induce microtubule stabilization and paclitaxel sensitization. Experimental Design: Fourteen kinases known to regulate paclitaxel sensitivity were depleted individually in 12 well-characterized ovarian cancer cell lines before measuring proliferation in the presence or absence of paclitaxel. Similar studies were performed by depleting all possible pairs of kinases in 6 ovarian cancer cell lines. Pairs that enhanced paclitaxel sensitivity across multiple cell lines were studied in depth in cell culture and two xenograft models. Results: Transfection of siRNA against 10 of the 14 kinases enhanced paclitaxel sensitivity in at least 6 of 12 cell lines. Dual knockdown of IKBKB/STK39 or EDN2/TBK1 enhanced paclitaxel sensitivity more than silencing single kinases. Sequential knockdown was superior to concurrent knockdown. Dual silencing of IKBKB/STK39 or EDN2/TBK1 stabilized microtubules by inhibiting phosphorylation of p38 and MAP4, inducing apoptosis and blocking cell cycle more effectively than silencing individual kinases. Knockdown of IKBKB/STK39 or EDN2/TBK1 enhanced paclitaxel sensitivity in two ovarian xenograft models. Conclusions: Sequential knockdown of dual kinases increased microtubule stability by decreasing p38-mediated phosphorylation of MAP4 and enhanced response to paclitaxel in ovarian cancer cell lines and xenografts, suggesting a strategy to improve primary therapy.
https://ift.tt/2MIRbr4
Comparison of longitudinal CA125 algorithms as a first line screen for ovarian cancer in the general population
Purpose: In the United Kingdom Collaborative Trial of Ovarian Cancer Screening(UKCTOCS) women in the multimodal (MMS) arm had a serum CA125 test (first-line), with those at increased risk, having repeat CA125/ultrasound (second-line test). CA125 was interpreted using the 'Risk of Ovarian Cancer Algorithm'(ROCA). We report on performance of other serial algorithms and a single CA125 threshold as a first line screen in the UKCTOCS dataset. Experimental Design: 50,083 post-menopausal women who attended 346,806 MMS screens were randomly split into training and validation sets, following stratification into cases (ovarian/tubal/peritoneal cancers) and controls. The two longitudinal algorithms, a new serial algorithm, method of mean trends (MMT) and the parametric empirical Bayes (PEB) were trained in the training set and tested in the blinded validation set and the performance characteristics, including that of a single CA125 threshold, were compared. Results The area under receiver operator curve (AUC) was significantly higher (p=0.01) for MMT (0.921) compared to CA125 single threshold (0.884). At a specificity of 89.5%, sensitivities for MMT (86.5%;95%CI:78.4-91.9) and PEB (88.5%;95%CI:80.6-93.4) were similar to that reported for ROCA (sensitivity 87.1%; specificity 87.6%; AUC 0.915) and significantly higher than the single CA125 threshold (73.1%;95%CI:63.6-80.8). Conclusions: These findings from the largest available serial CA125 data set in the general population provide definitive evidence that longitudinal algorithms are significantly superior to simple cut-offs for ovarian cancer screening. Use of these newer algorithms requires incorporation into a multimodal strategy. The results highlight the importance of incorporating serial change in biomarker levels in cancer screening/early detection strategies.
https://ift.tt/2KGO2e8
The Importance of the Double Product in the Six-Minute Walk Test to Predict Myocardial Function
Introduction. The Six-Minute Walk Test (6MWT) is a widely used test to measure the physical performance of patients to assess the effectiveness of treatment, to qualify for rehabilitation, and to evaluate its effects.. Aim. This paper focuses on the assessment of the growth of a double product (DP) during the 6MWT and its diagnostic value in the assessment of patients with heart failure. Material and Methods. The paper has retrospective character. We analyzed medical records of 412 patients hospitalized for cardiac reasons, in whom a 6MWT was performed. The patients were divided into two groups: one with diagnosed heart failure and a control group. Results. The patients with diagnosed heart failure, compared to the control group, were characterized by a shorter walking distance and greater DP increase at equal walking intervals. After distinguishing the group with the preserved and decreased left ventricle ejection fraction, the value of the DP increase was still higher compared to the control group. The mean DP increase corresponding to one meter of walk was the only one that correlated negatively with the left ventricular ejection fraction. Conclusion. The assessment of the increase of the DP during the march test seems to be a better parameter reflecting the efficiency of the myocardium from the distance of the march.
https://ift.tt/2lPqSUN
The Social Media Index as an Indicator of Quality for Emergency Medicine Blogs: A METRIQ Study
Online educational resources such as blogs are increasingly used for education by emergency medicine clinicians. The Social Media Index was developed to quantify their relative impact. The Medical Education Translational Resources: Indicators of Quality (METRIQ) study was conducted in part to determine the association between the Social Media Index score and quality as measured by gestalt and previously derived quality instruments.
https://ift.tt/2u28Tyc
Emergency Department Urinary Tract Infections Caused by Extended-Spectrum β-Lactamase–Producing Enterobacteriaceae: Many Patients Have No Identifiable Risk Factor and Discordant Empiric Therapy Is Common
Community-onset urinary tract infections (UTIs) caused by extended-spectrum β-lactamase (ESBL)–producing Enterobacteriaceae, which are resistant to ceftriaxone and usually coresistant to fluoroquinolones, are increasing worldwide. We investigate and describe in detail UTIs caused by ESBL-producing Enterobacteriaceae in our emergency department (ED), and determine the proportion that occurred in patients without health care–associated risk factors and who received discordant initial antibiotic therapy.
https://ift.tt/2lTaJh0
Point-of-Care Ultrasound Assessment of Bladder Fullness for Female Patients Awaiting Radiology-Performed Transabdominal Pelvic Ultrasound in a Pediatric Emergency Department: A Randomized Controlled Trial
Radiology-performed transabdominal pelvic ultrasound, used to evaluate female patients with suspected pelvic pathology in the pediatric emergency department (ED), is often delayed by the need to fill the bladder. We seek to determine whether point-of-care ultrasound assessment of bladder fullness can predict patient readiness for transabdominal pelvic ultrasound more quickly than patient sensation of bladder fullness.
https://ift.tt/2lTaBhw
Selection of Intravenous Fluids
Fluid resuscitation is one of the mainstays of shock management, and there has been tremendous interest in the choice of intravenous fluids. At present, isotonic crystalloid solutions are favored over colloid solutions. Among isotonic crystalloid solutions, 0.9% "normal" saline solution is perhaps the most widely prescribed medication in the United States. In clinical practice, the alternatives to 0.9% saline solution are balanced salt solutions that contain a physiologic amount of chloride and lactate or acetate as the base equivalent (Table 1).
https://ift.tt/2lSTs7V
Child and Parental Perspectives on Communication and Decision Making in Pediatric CKD: A Focus Group Study
Effective communication and shared decision making improve quality of care and patient outcomes but can be particularly challenging in pediatric chronic disease because children depend on their parents and clinicians to manage complex health care and developmental needs. We aimed to describe the perspectives of children with chronic kidney disease (CKD) and their parents with regard to communication and decision making.
https://ift.tt/2z8D24N
A foodborne acute gastroenteritis outbreak caused by GII.P16-GII.2 norovirus in a boarding high school, Beijing, China: a case–control study
In December 2017, an acute gastroenteritis outbreak involving 61 students occurred in a boarding high school in Beijing, China. We conducted an outbreak investigation immediately in order to determine the caus...
https://ift.tt/2KsQmpC
Coping strategies among nurses in South-west Ethiopia: descriptive, institution-based cross-sectional study
This study aimed to describe coping strategies for job stress among nurses working in Jimma Zone public hospitals, South-west Ethiopia. The study conducted from March to April 2014 through census using English...
https://ift.tt/2IPMcmb
Student performance on the Test of Scientific Literacy Skills (TOSLS) does not change with assignment of a low-stakes grade
Response-validated multiple-choice assessments are used in college courses to assess student learning gains. The ability of a test to accurately reflect student learning gains is highly dependent on the studen...
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Sero-prevalence of hepatitis B virus infection and associated factors among health care workers and medical waste handlers in primary hospitals of North-west Ethiopia
The aim of this cross-sectional study was to determined the sero-prevalence of HBV infection and associated factors among health care workers and medical waste handlers in primary hospitals of North-west Ethio...
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Modes of mechanical ventilation vary between hospitals and intensive care units within a university healthcare system: a retrospective observational study
As evidence-based guidance to aid clinicians with mechanical ventilation mode selection is scant, we sought to characterize the epidemiology thereof within a university healthcare system and hypothesized that ...
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T-Cell Receptor Excision Circles in HIV-Exposed, Uninfected Newborns Measured During a National Newborn Screening Program for Severe Combined Immunodeficiency
Severe combined immunodeficiency screening by measuring T-receptor excision circles at birth allows evaluation of the impact of various maternal conditions on newborn immunity. The slight decrease observed in a French cohort of newborns to HIV-infected mothers can be explained by the confounding factors of prematurity and African descent.
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Association Between Video Laryngoscopy and Adverse Tracheal Intubation-Associated Events in the Neonatal Intensive Care Unit
The effect of video laryngoscopy on adverse events during neonatal tracheal intubation is unknown. In this single site retrospective cohort study, video laryngoscopy was independently associated with decreased risk for adverse events during neonatal intubation.
https://ift.tt/2IPYqLm
Predictor of multidrug resistant tuberculosis in southwestern part of Ethiopia: a case control study
Curable disease tuberculosis is becoming incurable or difficult to treat due to drug resistance. Multi drug resistance tuberculosis is a major health problem for less developed countries. Development of drug r...
https://ift.tt/2KybfQB
Gliadin effect on the oxidative balance and DNA damage: An in-vitro, ex-vivo study
Gliadins are involved in gluten-related disorders and are responsible for the alteration of the cellular redox balance. It is not clear if the gliadin-related oxidative stress can induce DNA damage in enterocytes.
https://ift.tt/2NpmoRx
Neuropilin-1 (NRP-1) upregulated by IL-6/STAT3 signalling contributes to invasion in pancreatic neuroendocrine neoplasms
Although the upregulation of Neuropilin-1 (NRP-1) is associated with many solid tumours, its role in pancreatic neuroendocrine neoplasms (pNEN) has not been well elucidated. The aim of this study was to investigate the role of NRP-1 in improving treatment and determining the prognosis of pNEN. In this study, the expression of NRP-1 in pNEN tissue samples and pNEN cell line BON1 was analysed by Western blot, polymerase chain reaction (PCR) and immunocytochemistry upon exposure to interleukin-6 (IL-6).
https://ift.tt/2lQaun1
Conflicting IDH1/IDH2, ATRX, and 1p/19q results in diffuse gliomas: a commentary
Valeria Barresi, MD, PhD vbarresi@unime.it (See Fig..)
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Elevated DSN1 expression is associated with poor survival in patients with hepatocellular carcinoma
Dosage suppressor of Nnf1 (DSN1) is a component of the kinetochore protein complex that is required for proper chromosome segregation. Some studies have explored that DSN1 is related to colorectal cancer progression. However, the role of DSN1 in hepatocellular carcinoma (HCC) remains unknown. This study aimed to explore DSN1 expression in HCC tissues and investigated DSN1 functions in HCC cells. We obtained data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) to analyze DSN1 expression in HCC.
https://ift.tt/2lT1Hk6
Clonality assessment of multifocal lung adenocarcinoma by pathology evaluation and molecular analysis
The aim of this study was to explore morphologic and molecular features distinguishing between multifocal lung adenocarcinoma (MLA) and intrapulmonary metastases (IM). Sixteen patients with MLAs, a total of 34 tumors, were reviewed. Four approaches were used: (1) array-comparative genomic hybridization (CGH) as a standard clonality assessment; (2) EGFR and KRAS mutational profiles as a supplementary method; (3) comprehensive histologic assessment (CHA) was method I in pathology evaluation; (4) CHA combined with lepidic (scaling) component analysis was method II.
https://ift.tt/2lTIv5W
IgA dominant glomerulonephritis with a membranoproliferative pattern of injury
IgA dominant membranoproliferative glomerulonephritis (MPGN) is a descriptive term for renal biopsies in which differential diagnoses of unusual IgA nephropathy (IgAN), infection-related GN, or other etiologies are considered. We sought to understand clinical and pathologic features of this finding. Native kidney biopsies with IgA dominant immune deposits and diffuse MPGN features without significant exudative features or subepithelial deposits were retrospectively reviewed. Two groups (n=27, 33 biopsies) were identified, patients with chronic liver disease and those without.
https://ift.tt/2lPYW2Y
Exploration of BRAFV600E as a diagnostic adjuvant in the non-invasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP)
The non-invasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) and encapsulated follicular variant of papillary thyroid carcinoma (EFVPTC) are distinguished from classical papillary thyroid carcinoma with predominantly follicular architecture (CPTCPFA) by distinct histomorphologic and molecular features. CPTCPFA frequently harbor the oncogenic variant BRAFV600E while NIFTP and EFVPTC are largely RAS-driven. CPTCPFA have rare papillae and intranuclear pseudoinclusions that may distinguish them from NIFTP or EFVPTC.
https://ift.tt/2u2OZTP
Targeted gene sequencing of lynch syndrome-related and sporadic endometrial carcinomas
About one third of endometrial carcinomas (ECs), mainly of endometrioid histology, harbor the mismatch repair (MMR) defects and microsatellite instability (MSI). Among these, ECs arising in women with Lynch Syndrome (LS) account for a large proportion. To date, no somatic genetic analyses have been published comparing LS-ECs with sporadic ECs. In this work, we examined the mutational profiles of a well-characterized series of sporadic and LS-related ECs, performing exonic targeted sequencing of 16 genes mainly involved in MSI ECs.
https://ift.tt/2z7Lz7T
Association between Mismatch Negativity and Voxel-Based Brain Volume in Schizophrenia
Mismatch negativity (MMN), a type of event-related potential (ERP), is generated when a discernable change occurs in a series of repetitive standard stimuli (Naatanen et al., 1978). MMN represents the pre-attentive process of auditory discrimination and is associated with the function of auditory memory and involuntary attention shifting (Naatanen et al., 1979, Javitt et al., 1995, Naatanen et al., 2007). MMN deficit is extensively observed in schizophrenia patients (Shelley et al., 1991, Javitt et al., 1993, Umbricht et al., 2005).
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Reply to “Clinical Practice Guidelines or Clinical Research Guidelines?”
We thank our esteemed clinical colleagues Bagić et al. (2018) for their interest in our paper "IFCN-endorsed practical guidelines for clinical magnetoencephalography (MEG)" (Hari et al. 2018). We hereby respectfully respond to the criticism that our paper has (1) a confusing title and (2) poorly-weighted contents.
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Clinical Practice Guidelines or Clinical Research Guidelines?
We write in regard to the recent publication, "IFCN-endorsed practical guidelines for clinical magnetoencephalography (MEG)", by Hari et al. (2018) We find the title misleading and the content, though extensive, inadequate for guidelines in "clinical magnetoencephalography", as practiced. Our concerns follow:
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Successful liver-directed gene delivery by ERCP-guided hydrodynamic injection (with videos)
A simple, safe, targeted and efficient in vivo DNA delivery system is necessary for clinical-grade liver targeted gene therapy in humans. Intravascular hydrodynamic gene delivery has been investigated in large animal models but translation to humans has been hampered by its technical challenges, invasiveness and potential for significant cardiovascular adverse events. We posited that intrabiliary delivery of DNA plasmids via ERCP-guided hydrodynamic injection overcomes these obstacles.
https://ift.tt/2KK1RbJ
Impact of whole lung irradiation on survival outcome in patients with lung relapsed Ewing sarcoma
Whole lung irradiation (WLI) demonstrated potential improvements in survival outcomes in patients with isolated lung relapse of a Ewing sarcoma (EwS), and it additionally appears to be tolerated and safe for both pediatric and young adult patients. The primary tumor site and response of pulmonary lesions to chemotherapy are significant prognostic factors for survival following WLI. Without a randomized prospective analysis, the WLI should be considered for treating pulmonary relapsed EwS.
https://ift.tt/2z3LBO2
Hypertensive Disorders of Pregnancy and Maternal Cardiovascular Disease Risk Factor Development An Observational Cohort Study
https://ift.tt/2tZ7JDA
Women's Cardiovascular Health After a Hypertensive Disorder of Pregnancy
https://ift.tt/2Nik4eZ
Marijuana Use, Respiratory Symptoms, and Pulmonary Function A Systematic Review and Meta-analysis
https://ift.tt/2z5yTyq
Chemical composition, antioxidant, antimicrobial and Antiproliferative activities of essential oil of Mentha spicata L. (Lamiaceae) from Algerian Saharan atlas
Mentha spicata (M. spicata) is a member of Lamiaceae that spreads mainly in the temperate and sub-temperate zones of the world. It is considered as a rich source of essential oils, which is widely used in pharmac...
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10-hydroxy-2-decenoic acid of royal jelly exhibits bactericide and anti-inflammatory activity in human colon cancer cells
Royal jelly (RJ), the exclusive food for the larva of queen honeybee, is regarded as the novel supplement to promote human health. The function of RJ may be attributed to its major and unique fatty acid, 10-hy...
https://ift.tt/2KKZXV9
Reducing antibiotic use for uncomplicated urinary tract infection in general practice by treatment with uva-ursi (REGATTA) – a double-blind, randomized, controlled comparative effectiveness trial
Uncomplicated urinary tract infections (UTI) are common in general practice and usually treated with antibiotics. This contributes to increasing resistance rates of uropathogenic bacteria. A previous trial sho...
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Cytotoxic properties of the anthraquinone derivatives isolated from the roots of Rubia philippinensis
Cancer is one of the most frequently occurring diseases and is the second leading cause of death worldwide. In this study, anthraquinone derivatives (Compounds 1–5) were evaluated for their anti-cancer potenti...
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Antibacterial interactions, anti-inflammatory and cytotoxic effects of four medicinal plant species
The constant emergence of antibiotic resistant species and the adverse side effects of synthetic drugs are threatening the efficacy of the drugs that are currently in use. This study was aimed at investigating...
https://ift.tt/2NklDZE
Implications of Nine Risk Prediction Models for Selecting Ever-Smokers for Computed Tomography Lung Cancer Screening
https://ift.tt/2L0CDDf
Clinical Implications of Revised Pooled Cohort Equations for Estimating Atherosclerotic Cardiovascular Disease Risk
https://ift.tt/2Hhr7A9
ESPACOMP Medication Adherence Reporting Guideline (EMERGE)
Research on assessing or managing medication adherence applies approaches from observational, interventional, and implementation science that spans many disciplines and demands coherent conceptualization, valid methods, appropriate analyses, and complete and accurate reporting. To ensure such reporting, the European Society for Patient Adherence, COMpliance, and Persistence (ESPACOMP) Medication Adherence Reporting Guideline (EMERGE) recommends standard reporting approaches based on an accepted taxonomy.This guideline is derived from a literature review, a reactive Delphi study with 26 medication adherence experts from many countries and disciplines, and feedback from ESPACOMP members. It is designed to supplement existing guidelines for health research reporting and is structured around 4 minimum reporting criteria and 17 items reflecting best reporting practice. By enhancing and harmonizing research reporting, EMERGE aims to advance research and, ultimately, patient outcomes.
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Machine Learning and Evidence-Based Medicine
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Firearms and Dementia: Clinical Considerations
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Physician Burnout in the Electronic Health Record Era: Are We Ignoring the Real Cause?
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Defining, Estimating, and Communicating Overdiagnosis in Cancer Screening
The toll of inadequate health care is well-substantiated, but recognition is mounting that "too much" is also possible. Overdiagnosis represents one harm of too much medicine, but the concept can be confusing: It is often conflated with related harms (such as overtreatment, misclassification, false-positive results, and overdetection) and is difficult to measure because it cannot be directly observed. Because the U.S. Preventive Services Task Force (USPSTF) issues screening recommendations aimed largely at healthy persons, it has a particular interest in understanding harms related to screening, especially but not limited to overdiagnosis. In support of the USPSTF, the authors summarize the knowledge and provide guidance on defining, estimating, and communicating overdiagnosis in cancer screening. To improve consistency, thinking, and reporting about overdiagnosis, they suggest a specific definition. The authors articulate how variation in estimates of overdiagnosis can arise, identify approaches to estimating overdiagnosis, and describe best practices for communicating the potential for harm due to overdiagnosis.
https://ift.tt/2KmOScz
Identifying Patients for Whom Lung Cancer Screening Is Preference-Sensitive A Microsimulation Study
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Precision Screening for Lung Cancer: Risk-Based but Not Always Preference-Sensitive?
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Improving Implementation of Lung Cancer Screening With Risk Prediction Models
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When Given a Lemon, Make Lemonade: Revising Cardiovascular Risk Prediction Scores
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Group B streptococcal colonization in mothers and infants in western China: prevalences and risk factors
The epidemiology of maternal and infant Group B streptococcus (GBS) colonization is poorly understood in China. The aim of this study is to explore the prevalence and risk factors associated with maternal and ...
https://ift.tt/2KsFt7o
The inflammatory response and neuronal injury in Streptococcus suis meningitis
Many of the currently used models of bacterial meningitis have limitations due to direct inoculation of pathogens into the cerebrospinal fluid or brain and a relatively insensitive assessment of long-term sequ...
https://ift.tt/2MNHwzJ
Antibiotic resistance and molecular characterization of the hydrogen sulfide-negative phenotype among diverse Salmonella serovars in China
Among 2179 Salmonella isolates obtained during national surveillance for salmonellosis in China from 2005 to 2013, we identified 46 non-H2S-producing strains originating from different sources.
https://ift.tt/2KsFBno
Tuberculous pneumonia-induced severe ARDS complicated with DIC in a female child: a case of successful treatment
Tuberculous (TB) pneumonia can induce acute respiratory distress syndrome (ARDS). Although TB pneumonia is one of the causes of disease and death among children worldwide, the literature on TB pneumonia-induce...
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Does discovery of differentially culturable M tuberculosis really demand a new treatment paradigm? Longitudinal analysis of DNA clearance from sputum
According to the traditional tuberculosis (TB) treatment paradigm, the initial doses of treatment rapidly kill most Mycobacterium tuberculosis (Mtb) bacilli in sputum, yet many more months of daily treatment are ...
https://ift.tt/2KK8xXh
Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology
Here we present a protocol to visualize fine structures of peripheral nerves by obtaining and staining 1-2 µm sections with toluidine blue
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Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Circulating microRNAs have shown promise as biomarkers for cardiovascular diseases and acute myocardial infarctions. In this study, we describe a protocol for miRNA extraction, reverse transcription, and digital PCR for the absolute quantification of miRNAs in the serum of patients with cardiovascular disease.
https://ift.tt/2KNsglx
Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets
Beta-cell functionality is important for blood-glucose homeostasis, which is evaluated at single-cell resolution using a genetically encoded reporter for calcium influx.
https://ift.tt/2KsNQjf
Working >45 Hours Tied to Higher Diabetes Risk in Women
TUESDAY, July 3, 2018 -- For women, working 45 hours or more per week is associated with increased risk of diabetes, according to a study published online July 2 in BMJ Open Diabetes Research & Care. Mahée Gilbert-Ouimet, Ph.D., from the...
https://ift.tt/2tRzLSe
CVD Risk Up With Hypertensive Disorders of Pregnancy
TUESDAY, July 3, 2018 -- Women with hypertensive disorders of pregnancy (HDP) in their first pregnancy have increased rates of chronic hypertension, type 2 diabetes mellitus, and hypercholesterolemia, according to a study published online July 3 in...
https://ift.tt/2KwpYve
American Academy of Pediatrics Warns Against Using Fireworks
TUESDAY, July 3, 2018 -- The American Academy of Pediatrics (AAP) has issued a warning about fireworks safety. Fireworks can cause serious burns, blindness, scars, and death. Even sparklers can cause serious burns to users and bystanders, the AAP...
https://ift.tt/2MMTv0v
Smoking Marijuana May Be Tied to Cough, Sputum Production
TUESDAY, July 3, 2018 -- Smoking marijuana seems to be associated with increased risk of cough, sputum production, and wheezing, according to a review published online July 3 in the Annals of Internal Medicine. Mehrnaz Ghasemiesfe, M.D., from the...
https://ift.tt/2KwpRzO
Effective Marketing Methods Offered for Primary Care Doctors
TUESDAY, July 3, 2018 -- Marketing is important for physicians, and effective methods include internet marketing, speaking engagements, and print materials, according to an article published in Medical Economics. Marketing is increasingly important...
https://ift.tt/2MManV4
Dynamics of Brain Volume Loss Vary With MS Progression
TUESDAY, July 3, 2018 -- Brain volume loss (BVL) has nonlinear dynamics and limited reproducibility as a marker of therapeutic response in multiple sclerosis (MS), according to a study published online July 2 in JAMA Neurology. Magí Andorra, from...
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AMA Adopts Policy to Cut Sugar Sweetened Drink Consumption
TUESDAY, July 3, 2018 -- At the annual meeting of the American Medical Association (AMA), a policy was adopted to reduce the consumption of sugar-sweetened beverages (SSBs) as a way to reduce the amount of sugar that Americans consume. The new...
https://ift.tt/2MM3uTz
Automated Bone Scan Index Prognostic for Prostate Cancer
TUESDAY, July 3, 2018 -- The automated Bone Scan Index (aBSI) is an independent prognostic imaging biomarker of overall survival (OS) in metastatic castration-resistant prostate cancer (mCRPC), according to a study published online May 17 in JAMA...
https://ift.tt/2KwpyFa
Future Outlook Tied to Risk of Weapon Violence in Male Teens
TUESDAY, July 3, 2018 -- Positive future orientation is associated with reduced odds of weapon-related violence perpetration among teenage males from low-resource neighborhoods, according to a research letter published online July 2 in JAMA...
https://ift.tt/2ML898f
What to know about bone grafts
Doctors use bone grafts in the treatment of a variety of health issues, including fractures, bone infections, spinal fusions, tumors, and joint problems. In this article, learn about the different types of bone grafts and replacement materials. We also cover the possible risks, complications, and the recovery process.
https://ift.tt/2NpadDU
Small Talk May Not Be as Bad as Previously Thought
People who engage in more substantive conversations tend to be happier, according to research published in Psychological Science, a journal of the Association for Psychological Science. The research also shows that idle small talk is not necessarily negatively related to well-being, contrary to previous findings, said study coauthor Matthias Mehl, a professor of psychology at the University of Arizona.
The new research revisits a small study of 79 college students that Mehl published in 2010, which suggested that more meaningful conversations were linked to greater happiness, while more small talk was linked to unhappiness.
The new study, with a larger and more diverse sample size of 486 people, confirms that quality conversations are indeed linked to greater happiness, but found that small talk seems to have no relationship with happiness one way or the other.
The results hold true for both introverts and extroverts, Mehl said.
"We do not think anymore that there is an inherent tension between having small talk and having substantive conversations. Small talk didn't positively contribute to happiness, and it didn't negatively contribute to it," said Mehl, who carried out the work alongside lead author Anne Milek, who was a postdoctoral researcher in Mehl's lab from 2016 to 2017.
"With this study, we wanted to find out whether it is primarily the quantity or the quality of our social encounters that matters for one's well-being," said Milek, who is now a senior research scientist at the University of Zurich in Switzerland.
The researchers' findings are based on an analysis of four separate studies, in which snippets of audio were collected from participants' daily interactions. The study participants included college students; breast cancer survivors and their partners; recently divorced adults; and healthy adults participating in a meditation intervention.
Upon waking until bedtime, participants in each study wore what is called the EAR, or Electronically Activated Recording, device, which is set to turn on intermittently for short periods of time throughout the day to capture candid moments of daily interactions. The EAR was developed by Mehl at the UA to help psychologists capture behavioral data.
The research team coded the conversations recorded by the EAR to determine whether they were substantive.
"We define small talk as a conversation where the two conversation partners walk away still knowing equally as much – or little – about each other and nothing else," Mehl said. "In substantive conversation, there is real, meaningful information exchanged. Importantly, it could be about any topic – politics, relationships, the weather – it just needs to be at a more than trivial level of depth."
Study participants also completed surveys designed to measure their life satisfaction, as well as assess their personality.
Overall, study participants who engaged in a greater number of substantive conversations were happier, regardless of whether they had more introverted or extroverted personalities.
"We expected that personality might make a difference, for example that extroverts might benefit more from social interactions than introverts or that substantive conversations might be more closely linked to well-being for introverts than for extroverts, and were very surprised that this does not seem to be the case," Milek said.
"Conversation quantity and quality are related to well-being," said Mehl.
"We replicated that people who spend a lot of time alone are less satisfied with their lives and have lower well-being," Mehl said. "People who spend more time interacting and have more meaningful, substantive conversations are more satisfied. The happy life is social, rather than solitary, and meaningfully so."
Although small talk didn't have any direct link to participants' well-being, it may still be important, in that it can help lay the groundwork for more substantive conversations, Mehl said.
"I think of it like this: In every pill, there's an inactive ingredient, and it's a nice metaphor, because you cannot have the pill without the inactive ingredient," Mehl said. "We all understand that small talk is a necessary component to our social lives. You cannot usually walk up to a stranger and jump right into a deep, existential conversation because of social norms."
While the study establishes a link between substantive conversations and happiness, it's hard to say whether having more substantive conversations actually makes people happier, or if happier people have more substantive conversations, Mehl said. That's an area for future research.
"I would like to experimentally 'prescribe' people a few more substantive conversations," he said, "and see whether that does something to their happiness."
Coauthors on the research include Emily A. Butler, Allison M. Tackman, Deanna M. Kaplan, and David Sbarra of the University of Arizona; Charles L. Raison of the University of Wisconsin-Madison; and Simine Vazire of the University of California, Davis.
A. Milek's work on this article was supported by the Swiss National Science Foundation (P2ZHP1_164959). The research was supported by National Institutes of Health Grants 3R01AT004698, 5R01AT004698, R01HD069498, and R03CA137975.
All data and materials have been made publicly available via the Open Science Framework. The complete Open Practices Disclosure for this article is available online. This article has received badges for Open Data and Open Materials.
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Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
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heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
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