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Δευτέρα 1 Αυγούστου 2016
Issue Information: J. Biophotonics 8/2016
No abstract is available for this article.
http://ift.tt/2atYFhW
Front Cover: Wide-field time-gated photoluminescence microscopy for fast ultrahigh-sensitivity imaging of photoluminescent probes (J. Biophotonics 8/2016)
The ultimate limit of an imaging system based on photoluminescent molecular-probes is set by the signal-tonoise ratio between the bio-probes and the background. A microscopy technique which combines nanoruby photoluminescent bio-probes and time-gating acquisition is proposed in order to achieve manifold improvement on imaging contrast capabilities. The system is sensitive and fast enough to image single nanoruby particles within cultured cells as well as to capture the particle-binding kinetics in biological fluids.
Further details can be found in the article by Wan A. W. Razali et al. on pp. 848–858.
http://ift.tt/2ahB14V
Back Cover: Four-dimensional live imaging of hemodynamics in mammalian embryonic heart with Doppler optical coherence tomography (J. Biophotonics 8/2016)
Detailed volumetric measurement of hemodynamics in early developing mammalian heart can provide great insights for improved understanding of normal cardiogenesis and management of congenital cardiac disease. In this study, Doppler optical coherence tomography is performed in live mouse embryo culture to obtain the first four-dimensional high-resolution reconstruction and quantitative analysis of hemodynamic features in the mouse embryonic heart. This provides a powerful approach to investigate biomechanical regulation of early mammalian cardiogenesis.
Further details can be found in the article by Shang Wang et al. on pp. 837–847.
http://ift.tt/2atXLlD
Determination of serum glucose by isotope dilution liquid chromatography-tandem mass spectrometry: a candidate reference measurement procedure
Abstract
Accurate and precise glucose measurements are requisite for ensuring appropriate diagnosis and management of diseases related to hyperglycemia or hypoglycemia. It is necessary to have a higher order method to provide an accuracy base to which routine methods can be compared. We developed and evaluated a highly reliable measurement procedure based on isotope dilution liquid chromatography–tandem mass spectrometry (ID LC-MS/MS) with a simple one-step derivatization. An appropriate amount of serum was accurately weighed and spiked with an isotope-labeled internal standard. After protein precipitation, the supernatant was reacted with 1-phenyl-3-methyl-5-pyrazolone for chemical structural transformation. The glucose derivatives were analyzed with LC-MS/MS in positive electrospray ionization mode. The within-run and total CVs ranged from 0.28 to 0.42 % and from 0.42 to 0.76 %, respectively, for a concentration range of 1.691 to 15.676 mmol/L. A regression comparison of the presented method to an existing RMP based on ID GC-MS showed agreement with no statistical difference (Y = 0.9985X-0.008; 95 % CI for the slope, 0.9966 to 1.001; 95 % CI for the intercept, −0.012 to 0.019). The structural analogs of glucose with a molecular mass of 180 were tested, and no significant interference effect was found. The limit of quantification was estimated to 0.8 ng glucose in absolute amount. This method is accurate, simple, and can serve as a candidate reference measurement procedure (RMP) in the establishment of a serum glucose reference system.
http://ift.tt/2aqVILo
Extraction of toxic compounds from saliva by magnetic-stirring-assisted micro-solid-phase extraction step followed by headspace-gas chromatography-ion mobility spectrometry
Abstract
A new sample extraction procedure based on micro-solid-phase extraction (μSPE) using a mixture of sorbents of different polarities (polymeric reversed-phase sorbent HLB, silica-based sorbent C18, and multiwalled carbon nanotubes) was applied to extract benzene, toluene, butyraldehyde, benzaldehyde, and tolualdehyde present in saliva to avoid interference from moisture and matrix components and enhance sensitivity and selectivity of the ion mobility spectrometry (IMS) methodology proposed. The extraction of target analytes from saliva samples by using μSPE were followed by the desorption step carried out in the headspace vials placed in the autosampler of the IMS device. Then, 200 μL of headspace was injected into the GC column coupled to the IMS for its analysis. The method was fully validated in terms of sensitivity, precision, and recovery. The LODs and LOQs obtained, when analytes were dissolved in saliva samples to consider the matrix effect, were within the range of 0.38–0.49 and 1.26–1.66 μg mL−1, respectively. The relative standard deviations were <3.5 % for retention time and drift time values, which indicate that the method proposed can be applied to determine toxic compounds in saliva samples.
Graphical abstract
Summary of steps followed in the experimental set up of this work
http://ift.tt/2arZsPY
Forests, Vol. 7, Pages 167: Towards Harmonizing Leaf Litter Decomposition Studies Using Standard Tea Bags—A Field Study and Model Application
Decomposition of plant litter is a key process for the transfer of carbon and nutrients in ecosystems. Carbon contained in the decaying biomass is released to the atmosphere as respired CO2, and may contribute to global warming. Litterbag studies have been used to improve our knowledge of the drivers of litter decomposition, but they lack comparability because litter quality is plant species-specific. The use of commercial tea bags as a standard substrate was suggested in order to harmonize studies, where green tea and rooibos represent more labile and more recalcitrant C compounds as surrogates of local litter. Here we examine the potential of the use of standardized material for improving our understanding of litter decomposition across climate regions, and to further develop pertinent models. We measured the decomposition of incubated local and standard litters over two years along an elevation gradient in the Austrian Limestone Alps. The similar response to changes in temperature and precipitation of the pairs of local and standard litter—i.e., Fagus sylvatica and green tea, and Pinus nigra and rooibos tea, respectively—suggests the suitability of the standard litters for further examining the role of environmental drivers of decomposition. Harmonized data obtained from standardized litter experiments would provide a key prerequisite for further developing simulation models for the estimation of the C balance of ecosystem litter pools.
http://ift.tt/2afcopm
Shanghai Journal of Stomatology
. Year 2016 Issue 03 is now available on CAOD.
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Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
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heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
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