An optimised assay for quantitative, high-throughput analysis of polysialyltransferase activity

Analyst, 2016, Accepted Manuscript
DOI: 10.1039/C6AN01073C, Paper

Sara Elkashef, Mark Sutherland, L H Patterson, Paul Loadman, Robert Andrew Falconer
The polysialyltransferases are biologically important glycosyltransferase enzymes responsible for the biosynthesis of polysialic acid, a carbohydrate polymer that plays a critical role in the progression of several diseases, notably cancer....
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Fluorescence detection of single-nucleotide differences using aptamer-forming binary DNA probes

Analyst, 2016, Accepted Manuscript
DOI: 10.1039/C6AN00912C, Paper

Saori Kamoto, Masumi Hyuga, Teru Kato
We report a simple method for fluorescence detection of single-nucleotide alterations in a long target DNA, which is based on the formation of a three-way-junction-structured cholic-acid-binding DNA aptamer by the...
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Remote Sensing, Vol. 8, Pages 646: Maximizing the Diversity of Ensemble Random Forests for Tree Genera Classification Using High Density LiDAR Data

Recent research into improving the effectiveness of forest inventory management using airborne LiDAR data has focused on developing advanced theories in data analytics. Furthermore, supervised learning as a predictive model for classifying tree genera (and species, where possible) has been gaining popularity in order to minimize this labor-intensive task. However, bottlenecks remain that hinder the immediate adoption of supervised learning methods. With supervised classification, training samples are required for learning the parameters that govern the performance of a classifier, yet the selection of training data is often subjective and the quality of such samples is critically important. For LiDAR scanning in forest environments, the quantification of data quality is somewhat abstract, normally referring to some metric related to the completeness of individual tree crowns; however, this is not an issue that has received much attention in the literature. Intuitively the choice of training samples having varying quality will affect classification accuracy. In this paper a Diversity Index (DI) is proposed that characterizes the diversity of data quality (Qi) among selected training samples required for constructing a classification model of tree genera. The training sample is diversified in terms of data quality as opposed to the number of samples per class. The diversified training sample allows the classifier to better learn the positive and negative instances and; therefore; has a higher classification accuracy in discriminating the "unknown" class samples from the "known" samples. Our algorithm is implemented within the Random Forests base classifiers with six derived geometric features from LiDAR data. The training sample contains three tree genera (pine; poplar; and maple) and the validation samples contains four labels (pine; poplar; maple; and "unknown"). Classification accuracy improved from 72.8%; when training samples were selected randomly (with stratified sample size); to 93.8%; when samples were selected with additional criteria; and from 88.4% to 93.8% when an ensemble method was used.

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Biosensors, Vol. 6, Pages 43: Effects of Surface Epitope Coverage on the Sensitivity of Displacement Assays that Employ Modified Nanoparticles: Using Bisphenol A as a Model Analyte

With the ever-increasing use of nanoparticles in immunosensors, a fundamental study on the effect of epitope density is presented herein, with a small molecule epitope, on the performance of the displacement assay format in an enzyme-linked immunosorbent assay (ELISA). Thiolated bisphenol A (BPA) functionalized gold nanoparticles (cysBPAv-AuNPs) and specific anti-BPA antibodies are employed for this purpose. It is shown that the displacement of cysBPAv-AuNPs bound to the immobilized antibodies was influenced by both the avidity of bound cysBPAv-AuNPs and the concentration of free BPA to displace it. The importance of surface epitope density was that it changed the number of epitopes in close proximity to the antibody-binding site. This then influenced the avidity of cysBPAv-AuNPs bound to the immobilized antibody. Furthermore, the molar epitope concentration in an assay appears to affect the degree of antibody binding site saturation. Controlling surface epitope density of the functionalized nanoparticles and molar epitope concentration in an assay leads to a decrease of the concentration of free BPA required to displace the bound cysBPAv-AuNP, and hence better assay performance with regards to the D50 value and dynamic range in the displacement assay.

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Molecules, Vol. 21, Pages 1031: New Sesquiterpenenoids from Ainsliaea yunnanensis

Investigation of the ethanol extract of the whole plant of Ainsliaea yunnanensis led to the isolation of four new dimeric sesquiterpene lactones, ainsliadimer F–I (1–4), together with seven known dimeric sesquiterpene lactones (5–11) and ten sesquiterpenes (12–21). Their structures were elucidated by spectroscopic methods. The relative stereochemistry of ainsliadimer F was further confirmed by single crystal X-ray diffraction analysis. Compounds 1–21 were tested for the inhibition of nuclear factor kappa B (NF-κB) in the 293-NF-κB-luciferase reporter cell line induced by lipopolysaccharide (LPS), and Compounds 5, 18, 20 and 21 were further tested for the production of TNF-α, IL-1β, IL-6 and IL-10 in RAW 264.7 macrophages induced by LPS. Compounds 5, 18, 20 and 21 exhibited significant activity in anti-inflammatory activity assays.

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Polymers, Vol. 8, Pages 289: Key Role of Reinforcing Structures in the Flame Retardant Performance of Self-Reinforced Polypropylene Composites

The flame retardant synergism between highly stretched polymer fibres and intumescent flame retardant systems was investigated in self-reinforced polypropylene composites. It was found that the structure of reinforcement, such as degree of molecular orientation, fibre alignment and weave type, has a particular effect on the fire performance of the intumescent system. As little as 7.2 wt % additive content, one third of the amount needed in non-reinforced polypropylene matrix, was sufficient to reach a UL-94 V-0 rating. The best result was found in self-reinforced polypropylene composites reinforced with unidirectional fibres. In addition to the fire retardant performance, the mechanical properties were also evaluated. The maximum was found at optimal consolidation temperature, while the flame retardant additive in the matrix did not influence the mechanical performance up to the investigated 13 wt % concentration.

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IJERPH, Vol. 13, Pages 802: Effects of Long-Term Exposure to 60 GHz Millimeter-Wavelength Radiation on the Genotoxicity and Heat Shock Protein (Hsp) Expression of Cells Derived from Human Eye

Human corneal epithelial (HCE-T) and human lens epithelial (SRA01/04) cells derived from the human eye were exposed to 60 gigahertz (GHz) millimeter-wavelength radiation for 24 h. There was no statistically significant increase in the micronucleus (MN) frequency in cells exposed to 60 GHz millimeter-wavelength radiation at 1 mW/cm2 compared with sham-exposed controls and incubator controls. The MN frequency of cells treated with bleomycin for 1 h provided positive controls. The comet assay, used to detect DNA strand breaks, and heat shock protein (Hsp) expression also showed no statistically significant effects of exposure. These results indicate that exposure to millimeter-wavelength radiation has no effect on genotoxicity in human eye cells.

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IJMS, Vol. 17, Pages 1287: β-Ketoacyl-acyl Carrier Protein Synthase I (KASI) Plays Crucial Roles in the Plant Growth and Fatty Acids Synthesis in Tobacco

Fatty acids serve many functions in plants, but the effects of some key genes involved in fatty acids biosynthesis on plants growth and development are not well understood yet. To understand the functions of 3-ketoacyl-acyl-carrier protein synthase I (KASI) in tobacco, we isolated two KASI homologs, which we have designated NtKASI-1 and NtKASI-2. Expression analysis showed that these two KASI genes were transcribed constitutively in all tissues examined. Over-expression of NtKASI-1 in tobacco changed the fatty acid content in leaves, whereas over-expressed lines of NtKASI-2 exhibited distinct phenotypic features such as slightly variegated leaves and reduction of the fatty acid content in leaves, similar to the silencing plants of NtKASI-1 gene. Interestingly, the silencing of NtKASI-2 gene had no discernibly altered phenotypes compared to wild type. The double silencing plants of these two genes enhanced the phenotypic changes during vegetative and reproductive growth compared to wild type. These results uncovered that these two KASI genes had the partially functional redundancy, and that the KASI genes played a key role in regulating fatty acids synthesis and in mediating plant growth and development in tobacco.

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Energies, Vol. 9, Pages 622: The Energy and Environmental Performance of Ground-Mounted Photovoltaic Systems—A Timely Update

Given photovoltaics' (PVs) constant improvements in terms of material usage and energy efficiency, this paper provides a timely update on their life-cycle energy and environmental performance. Single-crystalline Si (sc-Si), multi-crystalline Si (mc-Si), cadmium telluride (CdTe) and copper indium gallium diselenide (CIGS) systems are analysed, considering the actual country of production and adapting the input electricity mix accordingly. Energy pay-back time (EPBT) results for fixed-tilt ground mounted installations range from 0.5 years for CdTe PV at high-irradiation (2300 kWh/(m2·yr)) to 2.8 years for sc-Si PV at low-irradiation (1000 kWh/(m2·yr)), with corresponding quality-adjusted energy return on investment (EROIPE-eq) values ranging from over 60 to ~10. Global warming potential (GWP) per kWhel averages out at ~30 g(CO2-eq), with lower values (down to ~10 g) for CdTe PV at high irradiation, and up to ~80 g for Chinese sc-Si PV at low irradiation. In general, results point to CdTe PV as the best performing technology from an environmental life-cycle perspective, also showing a remarkable improvement for current production modules in comparison with previous generations. Finally, we determined that one-axis tracking installations can improve the environmental profile of PV systems by approximately 10% for most impact metrics.

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Nutrients, Vol. 8, Pages 484: Sex-Specific Sociodemographic Correlates of Dietary Patterns in a Large Sample of French Elderly Individuals

This cross-sectional analysis provides up-to-date information about dietary patterns (DP) and their sociodemographic correlates in European elderly individuals. We studied 6686 enrollees aged 65+ (55% women) in the ongoing French population-based NutriNet-Santé e-cohort. Diet was assessed via three 24 h records. The sex-specific correlates of factor analysis derived DP were identified with multivariable linear regression. Using 22 pre-defined food groups, three DP were extracted. The "healthy" DP (fruit, vegetables, grains, nuts, fish) was positively associated with education, living alone, and being a former smoker (women), and negatively associated with being overweight, current smoker (men), age 75+ years, having hypertension, and obesity (women). The "western" DP (meat, appetizers, cheese, alcohol) was positively associated with BMI (men) and being a former/current smoker; it was negatively associated with age 75+ years (women) and living alone. The "traditional" DP (bread, potatoes, milk, vegetables, butter, stock) was positively associated with age and negatively associated with being a former/current smoker, education (men), and residing in an urban/semi-urban area. The findings support the diversity of DP among the elderly, highlighting sex-specific differences. The "healthy" DP explained the largest amount of variance in intake. Future studies could replicate the models in longitudinal and international contexts.

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Sensors, Vol. 16, Pages 1251: A Study of a Handrim-Activated Power-Assist Wheelchair Based on a Non-Contact Torque Sensor

Demand for wheelchairs is increasing with growing numbers of aged and disabled persons. Manual wheelchairs are the most commonly used assistive device for mobility because they are convenient to transport. Manual wheelchairs have several advantages but are not easy to use for the elderly or those who lack muscular strength. Therefore, handrim-activated power-assist wheelchairs (HAPAW) that can aid driving power with a motor by detecting user driving intentions through the handrim are being researched. This research will be on HAPAW that judge user driving intentions by using non-contact torque sensors. To deliver the desired motion, which is sensed from handrim rotation relative to a fixed controller, a new driving wheel mechanism is designed by applying a non-contact torque sensor, and corresponding torques are simulated. Torques are measured by a driving wheel prototype and compared with simulation results. The HAPAW prototype was developed using the wheels and a driving control algorithm that uses left and right input torques and time differences are used to check if the non-contact torque sensor can distinguish users' driving intentions. Through this procedure, it was confirmed that the proposed sensor can be used effectively in HAPAW.

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Sensors, Vol. 16, Pages 1254: Reversed Three-Dimensional Visible Light Indoor Positioning Utilizing Annular Receivers with Multi-Photodiodes

Exploiting the increasingly wide use of light emitting diodes (LEDs) lighting, in this paper we propose a reversed indoor positioning system (IPS) based on LED visible light communication (VLC) in order to improve indoor positioning accuracy. Unlike other VLC positioning systems, we employ two annular receivers with multi-photodiodes installed on the ceiling to locate the persons who carry LEDs. The basic idea is using multi-photodiodes to calculate the angle while using the received signal strength (RSS) method to calculate the distance. The experiment results show that the effective positioning range of the proposed system is 1.8 m when the distance between two receivers is 1.2 m. Moreover, a positioning error less than 0.2 m can be achieved under the condition that the radius of the PIN circle is between 0.16 m and 0.2 m, and the distance of the transmitter-receiver plane is less than 1.8 m, which will be effective in practice.

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IJERPH, Vol. 13, Pages 798: The Regularities in Insufficient Leisure-Time Physical Activity in Poland

Background: Insufficient physical activity (PA) has become an increasing risk factor of noncommunicable diseases and an important cause of deaths all over the world. The goal of this paper is to provide an in-depth description of insufficient PA in Poland as well as an examination of some of its correlates. Methods: We take advantage of statistical and econometric (logistic regression) analysis on the basis of a representative survey. Out of 3056 respondents, we analyze the 1260 low-PA ones. Results: The household size is more significant than the household life phase, and only several professions increase the odds of insufficient PA. The influence of socioeconomic status and place of residence is most robust. Gender does not significantly influence insufficient PA. Physical inactivity is concentrated among inhabitants of rural areas and town dwellers, with poor educational profile, and limited labor market opportunities. However, even high socioeconomic status does not completely prevent insufficient activity. Conclusions: Groups at the highest risk of inactivity should be covered by promotional actions first. Their aim should mainly be raising the leisure-time physical activity (LTPA) awareness. To start with, primary forms of activity would be walking, Nordic walking and jogging.

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Toxins, Vol. 8, Pages 235: A Rapid Assay to Detect Toxigenic Penicillium spp. Contamination in Wine and Musts

Wine and fermenting musts are grape products widely consumed worldwide. Since the presence of mycotoxin-producing fungi may greatly compromise their quality characteristics and safety, there is an increasing need for relatively rapid "user friendly" quantitative assays to detect fungal contamination both in grapes delivered to wineries and in final products. Although other fungi are most frequently involved in grape deterioration, secondary infections by Penicillium spp. are quite common, especially in cool areas with high humidity and in wines obtained by partially dried grapes. In this work, a single-tube nested real-time PCR approach—successfully applied to hazelnut and peanut allergen detection—was tested for the first time to trace Penicillium spp. in musts and wines. The method consisted of two sets of primers specifically designed to target the β-tubulin gene, to be simultaneously applied with the aim of lowering the detection limit of conventional real-time PCR. The assay was able to detect up to 1 fg of Penicillium DNA. As confirmation, patulin content of representative samples was determined. Most of analyzed wines/musts returned contaminated results at >50 ppb and a 76% accordance with molecular assay was observed. Although further large-scale trials are needed, these results encourage the use of the newly developed method in the pre-screening of fresh and processed grapes for the presence of Penicillium DNA before the evaluation of related toxins.

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IJERPH, Vol. 13, Pages 797: Quality of Life and Its Related Factors in Chinese Unemployed People: A Population-Based Cross-Sectional Study

With the global economic crisis and industrial restructuring, the unemployed are suffering from job loss-related stress and loss of income, which is believed to impair their mental and physical health, while coping and self-efficacy could combat the adverse effects of unemployment on health. Thus, this study aims to describe quality of life (QOL) among unemployed Chinese people and explore the associated factors. A cross-sectional study was conducted by convenience sampling, composed of 1825 unemployed people, from January 2011 to September 2011. Questionnaires pertaining to demographic characteristics, the 36-item Short-Form Health Survey (SF-36), the abbreviated version of the Cope Inventory (Brief COPE) and self-efficacy scales were used to collect information from unemployed people in the eastern, central, and western regions of China. Hierarchical multiple regression analysis was performed to explore the related factors of QOL. A structural equation model (SEM) was used to test the relations among coping, self-efficacy, and QOL. Mental QOL was significantly lower than physical QOL in Chinese unemployed people. Coping had significant effects on both physical component summary (PCS) and mental component summary (MCS), while self-efficacy played the mediating role in the association between Coping and QOL. Unemployed Chinese people's mental QOL was disrupted more seriously than their physical QOL. An increase in coping could improve QOL by promoting better management of issues brought about by unemployment. In addition, self-efficacy has the ability to reduce the impact of unemployment on QOL, through the mediating path of coping on QOL. This study highlights the need of coping skills training and self-efficacy enhancement for better management of unemployment in order to improve QOL and well-being.

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Buildings, Vol. 6, Pages 29: The Importance of Heating System Transient Response in Domestic Energy Labelling

European National Calculation Methods (NCM), such as the UK Standard Assessment Procedure (SAP), are used to make standardised and simplified assessments of building energy performance. These NCMs contain simplifications to aid ease of use and comparability of resulting Energy Performance Certificates (EPC). By comparing SAP with a modern, dynamic modelling system, this study quantifies internal temperatures and thereby heating energy consumption. Results show that for the considered test house SAP results correspond closely to a dynamic model using an idealistic heating system, with perfect control and instant responsiveness. However, the introduction of a dynamic, physically realistic gas fired boiler and water based heating system to the model results in a consistent increase in internal temperature (0.5 °C) and energy demand (by ca. 1000 kWh/a). Variation of further parameters within the dynamic model, controls and heat source size, are presented and compared to SAP results and assumptions. The inclusion of more realistic dynamics in building energy modelling for NCMs may provide a better basis for effective decision making with respect to a wide range of heating systems.

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Atmosphere, Vol. 7, Pages 104: Retention of Atmospheric Particles by Local Plant Leaves in the Mount Wutai Scenic Area, China

To evaluate the characteristics of atmospheric particle retention by plant leaves during the tourism season in Buddhism-based scenic areas, plants distributed in the core area of the Mount Wutai scenic area were selected for study: Populus davidiana (Po. davidiana), Rosa hugonis Hemsl. (R. hugonis), Betula platyphylla Suk. (B. platyphylla), Rosa xanthina Lindl. (R. xanthina), Periploca sepium Bunge (Pe. sepium), Spiraea salicifolia L. (S. salicifolia), Vitex negundo var. Heterophylla (V. negundo var. heterophylla) and Pinus tabuliformis Carrière (Pi. tabuliformis). Before rain, the atmospheric suspended particle-retaining weight of the plant leaves varied in the range of 6.95 ± 1.55 (Pi. tabuliformis) to 38.60 ± 18.32 mg/cm2 (Po. davidiana); the light shaded areas caused by particles on leaves were in the range of 7.25 ± 0.04 (Pi. tabuliformis) to 126.50 ± 6.66 cm2/leaf (Po. davidiana); and the atmospheric particle-retaining horizontal density of leaves varied in the range of 110 ± 2 (Pi. tabuliformis) to 255 ± 11 per cm2 (Po. davidiana). After rain, the atmospheric suspended particle-retaining quality of plant leaves varied in the range of 0.65 ± 0.23 (Pi. tabuliformis) to 3.50 ± 1.83 mg/cm2 (Po. davidiana); the light shaded areas by particles on leaves were in the range of 4.26 ± 0.02 (Pi. tabuliformis) to 45.96 ± 2.42 cm2/leaf (Po. davidiana); and the atmospheric particle-retaining horizontal density of leaves was in the range of 97 ± 2 (Pi. tabuliformis) to 147 ± 7 per cm2 (Po. davidiana). The broad-leaved plants, particularly Po. davidiana, R. hugonis and B. platyphylla, were appropriate species for purification of atmospheric particles. Plants with lower dust-retention abilities than the above three species (e.g., R. xanthina, Pe. sepium, S. salicifolia and V. negundo var. heterophylla) could be alternative plants for purification. However, the needle-leaved plant Pi. tabuliformis was not recommended as a tree species for purification of atmospheric particles in the core area of the Mount Wutai scenic area.

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IJGI, Vol. 5, Pages 140: Design and Implementation of a Robust Decision Support System for Marine Space Resource Utilization

Increasing coastal space resource utilization (CSRU) activities and their impact on coastal environments has been recognized as a critical coastal zone stressor. Consequently, the need for sustainable and valid CSRU management has been highlighted. In this study, a highly-intelligent prototype decision-aided system for CSRU was developed. In contrast with existing coastal decision-aided systems, this system is aimed at the management of CSRU, providing reliable and dynamic numerical simulation, analysis, and aided decision making for real coastal engineering based on a self-developed fully automatic numerical program. It was established on multi-tier distributed architecture based on Java EE. The most efficient strategies for spatial data organization, automatic coastal numerical programs, and impact assessment modules are demonstrated. In addition, its integrated construction involving the addition of a new coastal project on the webpage, its one-click numerical prediction of coastal environmental impacts, assessments based on numerical results, and its aided decision-making capabilities are addressed. The system was applied to Ningbo Sea, China, establishing the Ningbo CSRU Decision Support System. Two projects were demonstrated: one reclamation project and one land-based outlet planning case. Results indicated that these projects had detrimental effects on local coastal environments. Therefore, the approvals of these projects were not recommended.

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Education Sciences, Vol. 6, Pages 26: “We’re One Team”: Examining Community School Implementation Strategies in Oakland

The community school model posits that the traditional school model is not sufficient to overcome the role of poverty in equitable access to learning, and that improving student achievement requires addressing the needs of the whole child. By leveraging community partnerships to address student barriers to learning and shift relationships between schools, families, and community, the community school model represents an expanded vision of what schools are, who they include, and what they are responsible for. This paper aims to improve our understanding of community school implementation, based on qualitative research in five community schools in Oakland, California. We apply the Children's Aid Society's framework of four community school capacities including: (1) comprehensiveness; (2) collaboration; (3) coherence; and (4) commitment (Lubell, 2011) in our analysis. We find evidence of a collaborative culture, in which school and community partner staff worked together across traditional boundaries to serve students. Schools showed signs of coherence of vision and goals, and alignment of services and supports with the instructional core of the school. Community school strategies not only provided important school-based services but also represented an expansion of the traditional school model by leveraging and aligning community partners to improve student outcomes.

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Religions, Vol. 7, Pages 102: The Dilemmas of Monogamy: Pleasure, Discipline and the Pentecostal Moral Self in the Republic of Benin

Based on ethnographic research in the Republic of Benin, this article explores how Pentecostal teachings on marriage and the management of sexual pleasure contribute to shaping converts' moral selves. For Pentecostals, fidelity towards God, when single and fidelity between partners, once married, is presented as the ideal model of partnership to which every "Born-Again" should aspire. In the context where polygamous unions are socially accepted, Pentecostal pastors teach that a satisfactory sexual life restricted to marriage is the means of building successful monogamous unions. However, sexual satisfaction might not always guarantee marital success, especially when people face problems of infertility. The author suggests that the disciplinary regimes that these teachings promote contribute to shaping new modes of intimacy, which are compatible with societal changes but often contradict the extant social norms and ideals of reproduction. Moral dilemmas arising from this tension are the key to understanding how Pentecostal Christianity shapes the moral self. The article addresses how Pentecostals in Benin navigate and negotiate cultural continuities and discontinuities in relation to church authority and family life.

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Remote Sensing, Vol. 8, Pages 638: Estimation of Energy Balance Components over a Drip-Irrigated Olive Orchard Using Thermal and Multispectral Cameras Placed on a Helicopter-Based Unmanned Aerial Vehicle (UAV)

A field experiment was carried out to implement a remote sensing energy balance (RSEB) algorithm for estimating the incoming solar radiation (Rsi), net radiation (Rn), sensible heat flux (H), soil heat flux (G) and latent heat flux (LE) over a drip-irrigated olive (cv. Arbequina) orchard located in the Pencahue Valley, Maule Region, Chile (35°25′S; 71°44′W; 90 m above sea level). For this study, a helicopter-based unmanned aerial vehicle (UAV) was equipped with multispectral and infrared thermal cameras to obtain simultaneously the normalized difference vegetation index (NDVI) and surface temperature (Tsurface) at very high resolution (6 cm × 6 cm). Meteorological variables and surface energy balance components were measured at the time of the UAV overpass (near solar noon). The performance of the RSEB algorithm was evaluated using measurements of H and LE obtained from an eddy correlation system. In addition, estimated values of Rsi and Rn were compared with ground-truth measurements from a four-way net radiometer while those of G were compared with soil heat flux based on flux plates. Results indicated that RSEB algorithm estimated LE and H with errors of 7% and 5%, respectively. Values of the root mean squared error (RMSE) and mean absolute error (MAE) for LE were 50 and 43 W m−2 while those for H were 56 and 46 W m−2, respectively. Finally, the RSEB algorithm computed Rsi, Rn and G with error less than 5% and with values of RMSE and MAE less than 38 W m−2. Results demonstrated that multispectral and thermal cameras placed on an UAV could provide an excellent tool to evaluate the intra-orchard spatial variability of Rn, G, H, LE, NDVI and Tsurface over the tree canopy and soil surface between rows.

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Polymers, Vol. 8, Pages 285: Enhancing the Adhesive Strength of a Plywood Adhesive Developed from Hydrolyzed Specified Risk Materials

The current production of wood composites relies mostly on formaldehyde-based adhesives such as urea formaldehyde (UF) and phenol formaldehyde (PF) resins. As these resins are produced from non-renewable resources, and there are some ongoing issues with possible health hazard due to formaldehyde emission from such products, the purpose of this research was to develop a formaldehyde-free plywood adhesive utilizing waste protein as a renewable feedstock. The feedstock for this work was specified risk material (SRM), which is currently being disposed of either by incineration or by landfilling. In this report, we describe a technology for utilization of SRM for the development of an environmentally friendly plywood adhesive. SRM was thermally hydrolyzed using a Canadian government-approved protocol, and the peptides were recovered from the hydrolyzate. The recovered peptides were chemically crosslinked with polyamidoamine-epichlorohydrin (PAE) resin to develop an adhesive system for bonding of plywood specimens. The effects of crosslinking time, peptides/crosslinking agent ratio, and temperature of hot pressing of plywood specimens on the strength of formulated adhesives were investigated. Formulations containing as much as 78% (wt/wt) peptides met the ASTM (American Society for Testing and Materials) specifications of minimum dry and soaked shear strength requirement for UF resin type adhesives. Under the optimum conditions tested, the peptides–PAE resin-based formulations resulted in plywood specimens having comparable dry as well as soaked shear strength to that of commercial PF resin.

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Micromachines, Vol. 7, Pages 139: A Reconfigurable Microfluidics Platform for Microparticle Separation and Fluid Mixing

Microfluidics is an engineering tool used to control and manipulate fluid flows, with practical applications for lab-on-a-chip, point-of-care testing, and biological/medical research. However, microfluidic platforms typically lack the ability to create a fluidic duct, having an arbitrary flow path, and to change the path as needed without additional design and fabrication processes. To address this challenge, we present a simple yet effective approach for facile, on-demand reconfiguration of microfluidic channels using flexible polymer tubing. The tubing provides both a well-defined, cross-sectional geometry to allow reliable fluidic operation and excellent flexibility to achieve a high degree of freedom for reconfiguration of flow pathways. We demonstrate that microparticle separation and fluid mixing can be successfully implemented by reconfiguring the shape of the tubing. The tubing is coiled around a 3D-printed barrel to make a spiral microchannel with a constant curvature for inertial separation of microparticles. Multiple knots are also made in the tubing to create a highly tortuous flow path, which induces transverse secondary flows, Dean flows, and, thus, enhances the mixing of fluids. The reconfigurable microfluidics approach, with advantages including low-cost, simplicity, and ease of use, can serve as a promising complement to conventional microfabrication methods, which require complex fabrication processes with expensive equipment and lack a degree of freedom for reconfiguration.

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Sustainability, Vol. 8, Pages 765: Sustainability of Local and Global Food Chains: Introduction to the Special Issue

Sustainability assessment is one of the keys to competition by food supply chains over sustainability. The way it is conceived and embodied into decision-makers' choices affects the competitiveness of local and global chains. Science-based assessment methodologies have made substantial progress, but uncertainties—as well as interests at stake—are high. There are no science-based methods that are able to give an unchallenged verdict over the sustainability performance of a firm, let alone a supply chain. Assessment methods are more suited for medium-large firm dimensions, as planning, monitoring, and reporting are costly. Moreover, the availability of data affects the choice of parameters to be measured, and many claims of local food are not easily measurable. To give local chains a chance to operate on a level playing field, there is the need to re-think sustainability assessment processes and tailor them to the characteristics of the analysed supply chains. We indicate seven key points on which we think scholars should focus their attention when dealing with food supply chain sustainability assessment.

http://ift.tt/2b2wqc4

Sensors, Vol. 16, Pages 1252: Analysis of the Bias on the Beidou GEO Multipath Combinations

The Beidou navigation satellite system is a very important sensor for positioning in the Asia-Pacific region. The Beidou inclined geosynchronous orbit (IGSO) and medium Earth orbit (MEO) satellites have been analysed in some studies previously conducted by other researchers; this paper seeks to gain more insight regarding the geostationary earth orbit (GEO) satellites. Employing correlation analysis, Fourier transformation and wavelet decomposition, we validate whether there is a systematic bias in their multipath combinations. These biases can be observed clearly in satellites C01, C02 and C04 and have a great correlation with time series instead of elevation, being significantly different from those of the Beidou IGSO and MEO satellites. We propose a correction model to mitigate this bias based on its daily periodicity characteristic. After the model has been applied, the performance of the positioning estimations of the eight stations distributed in the Asia-Pacific region is evaluated and compared. The results show that residuals of multipath series behaves random noise; for the single point positioning (SPP) and precise point positioning (PPP) approaches, the positioning accuracy in the upward direction can be improved by 8 cm and 6 mm, respectively, and by 2 cm and 4 mm, respectively, for the horizontal component.

http://ift.tt/2bdFGXl

Information, Vol. 7, Pages 50: Smart Homes and Sensors for Surveillance and Preventive Education at Home: Example of Obesity

(1) Background: The aim of this paper is to show that e-health tools like smart homes allow the personalization of the surveillance and preventive education of chronic patients, such as obese persons, in order to maintain a comfortable and preventive lifestyle at home. (2) Technologies and methods: Several types of sensors allow coaching the patient at home, e.g., the sensors recording the activity and monitoring the physiology of the person. All of this information serves to personalize serious games dedicated to preventive education, for example in nutrition and vision. (3) Results: We built a system of personalized preventive education at home based on serious games, derived from the feedback information they provide through a monitoring system. Therefore, it is possible to define (after clustering and personalized calibration) from the at home surveillance of chronic patients different comfort zones where their behavior can be estimated as normal or abnormal and, then, to adapt both alarm levels for surveillance and education programs for prevention, the chosen example of application being obesity.

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The Diagnosis of Iliac Bone Destruction in Children: 22 Cases from Two Centres

Iliac bone destruction in children is uncommon and presents various imaging features. Correct diagnosis based on clinical and imaging features is difficult. This research aimed to retrospectively explore the clinical features, imaging, and histopathological diagnosis of children with iliac bone destruction. A total of 22 children with iliac bone destruction were enrolled in this retrospective analysis from two children's hospitals during July 2007 to April 2015. Clinical features, imaging, and histopathological findings were analysed. The mode of iliac bone destruction, lesion structure, and the relationship between the range of soft tissue mass and cortical destruction were determined based on imaging data. The data were analysed using descriptive methods. Of the iliac bone destruction cases, eight cases were neuroblastoma iliac bone metastasis, seven cases were bone eosinophilic granuloma, two cases were Ewing's sarcoma, two cases were osteomyelitis, one case was bone cyst, one case was bone fibrous dysplasia, and one case was non-Hodgkin's lymphoma. Iliac bone destruction varies widely in children. Metastatic neuroblastoma and eosinophilic granuloma are the most commonly involved childhood tumours.

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In Silico Prediction of Gamma-Aminobutyric Acid Type-A Receptors Using Novel Machine-Learning-Based SVM and GBDT Approaches

Gamma-aminobutyric acid type-A receptors (s) belong to multisubunit membrane spanning ligand-gated ion channels (LGICs) which act as the principal mediators of rapid inhibitory synaptic transmission in the human brain. Therefore, the category prediction of s just from the protein amino acid sequence would be very helpful for the recognition and research of novel receptors. Based on the proteins' physicochemical properties, amino acids composition and position, a classifier was first constructed using a 188-dimensional (188D) algorithm at 90% cd-hit identity and compared with pseudo-amino acid composition (PseAAC) and ProtrWeb web-based algorithms for human proteins. Then, four classifiers including gradient boosting decision tree (GBDT), random forest (RF), a library for support vector machine (libSVM), and k-nearest neighbor (-NN) were compared on the dataset at cd-hit 40% low identity. This work obtained the highest correctly classified rate at 96.8% and the highest specificity at 99.29%. But the values of sensitivity, accuracy, and Matthew's correlation coefficient were a little lower than those of PseAAC and ProtrWeb; GBDT and libSVM can make a little better performance than RF and -NN at the second dataset. In conclusion, a classifier was successfully constructed using only the protein sequence information.

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Sprinkling of neural dust opens door to electroceuticals

University of California Berkeley Health News

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Diagnosis accuracy of transcutaneous bilirubinometry in very preterm newborns

Neonatology

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Sofosbuvir plus daclatasvir with or without ribavirin for chronic hepatitis C infection: Impact of drug concentration on viral load decay

Digestive and Liver Diseases

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Prediction models of mortality in acute pancreatitis in adults: a systematic review

Annals of Internal Medicine

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Vitamin D deficiency in non-alcoholic fatty liver disease

Clinical Nutrition

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Methylation quantitative trait loci within the TOMM20 gene are associated with metabolic syndrome-related lipid alterations in severely obese subjects

Diabetology & Metabolic Syndrome

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Use of antibiotics among patients with cirrhosis and upper gastrointestinal bleeding is associated with reduced mortality

Clinical Gastroenterology and Hepatology

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A randomized, double-blind, placebo-controlled phase 2 study of brodalumab in patients with moderate-to-severe Crohn’s disease

The American Journal of Gastroenterology

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Validity and reliability of the Bristol Stool Form Scale in healthy adults and patients with diarrhoea-predominant irritable bowel syndrome

Alimentary Pharmacology and Therapeutics

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Role of conserved E2 residue W420 in receptor binding and hepatitis C virus infection

Journal of Virology

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Risk of gastric cancer among patients with intestinal metaplasia of the stomach in a United States integrated healthcare system

Clinical Gastroenterology and Hepatology

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Relation of serum irisin level with metabolic and antropometric parameters in obese children

Journal of Diabetes and its Complications

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Association between results of a gene expression signature assay and recurrence-free interval in patients with stage II colon cancer in cancer and leukemia group B 9581

Journal of Clinical Oncology

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A case of acute esophageal necrosis and duodenal disease in a patient with adrenal insufficiency

Clinical Gastroenterology and Hepatology

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Epidemiology of hepatitis C virus and genotype distribution in immigrants crossing to Europe from north and sub-Saharan Africa

Travel Medicine and Infectious Disease

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AGA establishes NIH-funded registry to track fecal microbiota transplants

American Gastroenterological Association News

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Sensors, Vol. 16, Pages 1249: Comparative Study of Two Different TiO2 Film Sensors on Response to H2 under UV Light and Room Temperature

An anatase TiO2 film sensor was prepared by a facile in-situ method on the interdigitated Au electrode deposited on the alumina substrate. The structure, morphology and the optical properties of the in-situ TiO2 film sensor were characterized by X-ray diffraction, Scanning Electron Microscopy, and UV-vis diffuse reflectance spectra. The photo-assisted gas sensitivities of the prepared film towards H2 gas were evaluated at room temperature in N2 and synthetic air atmospheres. As compared to TiO2 film sensor prepared by drop-coating method, this in-situ TiO2 film sensor exhibited a more compact structure composed of uniform TiO2 microspheres as well as a better gas sensitivity towards H2 under UV irradiation, especially in synthetic air. The photo-electrochemical measurements suggest that these improvements may be associated with the efficient charge transfer in the TiO2 interface induced by the TiO2 microsphere structure. This study might offer a feasible approach to develop photo-assisted gas sensors at ambient temperature.

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Sustainability, Vol. 8, Pages 767: Research of Sustainable Use of Tire Shreds in Landfill

Studies are on-going to establish the suitability of tire shreds in the landfill bottom drainage layer, to minimize clogging. Four experimental columns were constructed in which were 500 mm long and 200 mm in diameter. They were filled with different fillers and with a different amount of tire shreds. The most important problem with drainage filler in landfills is clogging. Over long periods of time in landfill operation, the drainage layer clogs (the pores of the layer become smaller and the porosity of the layer becomes smaller). The experiment was carried out for 365 days. Although the landfill for this period represents only one-fiftieth or less of the operation time, the laboratory tests found that the drainage layer bandwidth of reduction in one year can have a negative impact in the long run over time. The main elements that influence the decrease of conductivity are the total suspended solids and calcium and iron compounds. The change of these compounds was observed during the column study, where the concentration of each month in all the columns was decreasing. The results showed that the waste of rubber (tire shreds) used for creating fillers in columns provided greater porosity of the layer. It is a beneficial reason to use a rubble and tire shreds waste mix for forming the drainage layer in landfills.

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Mathematics, Vol. 4, Pages 50: Complete Classification of Cylindrically Symmetric Static Spacetimes and the Corresponding Conservation Laws

In this paper we find the Noether symmetries of the Lagrangian of cylindrically symmetric static spacetimes. Using this approach we recover all cylindrically symmetric static spacetimes appeared in the classification by isometries and homotheties. We give different classes of cylindrically symmetric static spacetimes along with the Noether symmetries of the corresponding Lagrangians and conservation laws.

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IJGI, Vol. 5, Pages 139: Spatiotemporal Modeling of Urban Growth Predictions Based on Driving Force Factors in Five Saudi Arabian Cities

This paper investigates the effect of four driving forces, including elevation, slope, distance to drainage and distance to major roads, on urban expansion in five Saudi Arabian cities: Riyadh, Jeddah, Makkah, Al-Taif and Eastern Area. The prediction of urban probabilities in the selected cities based on the four driving forces is generated using a logistic regression model for two time periods of urban change in 1985 and 2014. The validation of the model was tested using two approaches. The first approach was a quantitative analysis by using the Relative Operating Characteristic (ROC) method. The second approach was a qualitative analysis in which the probable urban growth maps based on urban changes in 1985 is used to test the performance of the model to predict the probable urban growth after 2014 by comparing the probable maps of 1985 and the actual urban growth of 2014. The results indicate that the prediction model of 2014 provides a reliable and consistent prediction based on the performance of 1985. The analysis of driving forces shows variable effects over time. Variables such as elevation, slope and road distance had significant effects on the selected cities. However, distance to major roads was the factor with the most impact to determine the urban form in all five cites in both 1985 and 2014.

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IJGI, Vol. 5, Pages 138: Occlusion-Free Visualization of Important Geographic Features in 3D Urban Environments

Modern cities are dense with very tall buildings, which often leads to features of interest (FOIs, e.g., relevant roads and associated landmarks) being occluded by clusters of buildings. Thus, from any given point of view, users can see only a small area of the city. However, it is currently an important technical problem to maintain the visibility of FOIs while preserving the urban shapes and spatial relationships between features. In this paper, we present a novel automatic visualization method to generate occlusion-free views for FOIs in real time. Our method integrates with three effective cartographic schemes: route broadening, building displacement, and building scaling, using an optimization framework A series of distortion energies are presented to preserve the urban resemblance, considering the view position and the urban features based on spatial cognition to maintain spatial and temporal coherence. Our approach can be used to visualize large urban environments at interactive framerates in which the visibility of the occluded FOIs is maximized while the deformation of the landscape's shape is minimized. Using this approach, the visual readability of such 3D urban maps can be much improved.

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Life, Vol. 6, Pages 32: Ultra Large Gene Families: A Matter of Adaptation or Genomic Parasites?

Gene duplication is an important mechanism of molecular evolution. It offers a fast track to modification, diversification, redundancy or rescue of gene function. However, duplication may also be neutral or (slightly) deleterious, and often ends in pseudo-geneisation. Here, we investigate the phylogenetic distribution of ultra large gene families on long and short evolutionary time scales. In particular, we focus on a family of NACHT-domain and leucine-rich-repeat-containing (NLR)-genes, which we previously found in large numbers to occupy one chromosome arm of the zebrafish genome. We were interested to see whether such a tight clustering is characteristic for ultra large gene families. Our data reconfirm that most gene family inflations are lineage-specific, but we can only identify very few gene clusters. Based on our observations we hypothesise that, beyond a certain size threshold, ultra large gene families continue to proliferate in a mechanism we term "run-away evolution". This process might ultimately lead to the failure of genomic integrity and drive species to extinction.

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