Functional Analysis of the Voltage Sensor Domain Present in the Mammalian Sperm-Specific NA+/H+ Exchanger by Patch-Clamp Fluorometry of Chimeric Fluorescent Voltage Sensor

Several enzymes, transporters and ion channels accurately regulate sperm motility. Some of these proteins are expressed only in spermatozoa, but the functional relationship among them is not fully understood. Sperm-specific Na+/H+ exchanger (sNHE) is an essential protein for mouse sperm motility regulation and is localized in the principal piece of mammalian spermatozoa. Differently from somatic NHEs, sNHE has two predicted regulatory domains, a voltage sensor domain (VSD) and a cyclic nucleotide binding domain (CNBD), although their functionality remains to be confirmed.

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