Imaging of bioluminescent Klebsiella pneumoniae induced pulmonary infection in an immunosuppressed mouse model: Journal of International Medical Research, Volume 48, Issue 10, October 2020.
ObjectiveTo establish a mouse model of bioluminescent Klebsiella pneumoniae-induced lung infection, under different infection states after pretreatment with various dosages of cyclophosphamide (CTX).MethodsA K. pneumoniae strain carrying the luxCDABE operon was used to infect immunocompetent mice (intraperitoneal injection of saline at 4 days and 1 day prior to experimental lung infection) and immunodeficient mice (50 mg/kg CTX at 4 days and 50 mg/kg CTX at 1 day prior to lung infection; or 150 mg/kg CTX at 4 days and 100 mg/kg CTX at 1 day prior to lung infection). Disease progression was monitored in living mice using a bioluminescence imaging system. The bioluminescent images, bacterial loads in lungs, blood cytological changes and histopathology of lungs were analysed.ResultsK. pneumoniae-induced lung infection models were established in mice pretreated with CTX. Different doses of CTX led to different severities of lung infection. Mice pretreated with 150/100 mg/kg CTX were more suitable for real-time monitoring as they had more typical bioluminescent images of lung infection, more obvious changes in the bioluminescent intensity values, more bacterial colonies in the lungs and more distinct pulmonary pathological changes.ConclusionsA stable bioluminescent K. pneumonia-induced lung infection model was successfully established in mice pretreated with CTX, which can be semi-quantitatively monitored in real-time.
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
▼
2020
(1624)
- ► Δεκεμβρίου (293)
-
▼
Οκτωβρίου
(341)
-
▼
Οκτ 12
(80)
- Medicine by Alexandros G. Sfakianakis
- pubmed: carotid blowout synd...
- pubmed: gene therapies for h...
- ScienceDirect Publication: Biomaterials
- Neurocutaneous melanocytosis (melanosis)
- What is dimethoate used for? Dimethoate is used to...
- Organophosphate Poisoning PDF
- Partial internal biliary diversion
- Biliary ascariasis and severe bacterial outcomes
- The Fussy Baby Network® (FBN), a program supportin...
- Birth Weight and the Development of Functional Gas...
- Low prevalence of infantile functional gastrointes...
- DNA repair targeted therapy
- Plants, Vol. 9, Pages 1350: Identification and An...
- Clinical Genetics
- Intralesional methotrexate
- Intralesional immunotherapy for molluscum contagiosum
- Standardizing a volume benchmark for cerebrospinal...
- Adenomatous neuroendocrine tumors of the middle ea...
- Management of transmodiolar and transmacular cochl...
- Association of Estrogen Receptor 1 and Tumor Necro...
- Primary Intraosseous Squamous Cell Carcinoma Invol...
- Lymphocyte predominant Hodgkin lymphoma, antigen‐d...
- SETDB1 promotes gastric carcinogenesis and metasta...
- Microglia/macrophages express alternative proangio...
- Evaluation of myostatin as a possible regulator an...
- Effect of etelcalcetide on parathyroid hormone sec...
- The role of immune checkpoint inhibitor therapy in...
- Translational perspectives to treat Epidermolysis ...
- Cantilevered resin-bonded fixed partial dentures
- Telomerase reverse transcriptase rs2736098 polymor...
- Cardiac contractility modulation attenuates struct...
- Imaging of bioluminescent Klebsiella pneumoniae in...
- Serum oestradiol levels are inversely associated w...
- Large (stage ≥T2b) and locally advanced renal cell...
- Risk factors for early postoperative recurrence in...
- Immune dysfunction in MGAT2‐CDG
- Cutaneous Manifestations in Adult Patients with CO...
- The Role of Environmental Exposures in Atopic Derm...
- Strategies for having a more effective and less to...
- Mutationssignaturen beim Kopf- und Hals-Tumor
- Frameless stereotactic radiosurgery
- Custom designed radial forearm free flap for recon...
- Spindle Cell Melanoma Presenting as an Ulcer in a ...
- Nummular Headache
- Bleomycin sclerotherapy in lymphangiomas of the he...
- Adjuvant radiotherapy for endometrial cancer with ...
- DDGP vs. SMILE in Relapsed/Refractory Extranodal N...
- Remimazolam in Procedural Sedation With Nonhomogen...
- Evaluation of the Electroglottographic Signal Vari...
- Denosumab induced atypical fracture of free vascul...
- Renal cell carcinoma metastasis to the maxillary b...
- Driveline Infection with Vacuum-Assisted Closure T...
- Conservative Treatment of Iatrogenic Ascending Aor...
- Genome-enabled inference of functional genetic var...
- The Effect of Pituitary Gland Disorders on Glucose...
- Effect of periosteal reaction in medication-relate...
- A combined approach to correct posterior left vent...
- Synonymous mutations and the molecular evolution o...
- Serial circulating tumor DNA in predicting and mon...
- Ramucirumab in Combination with Osimertinib in Adv...
- Lysine-Specific Demethylase-1 Inhibitor CC-90011 i...
- Three distinct stroma types in human pancreatic ca...
- Activity and Safety of Geptanolimab (GB226) for pa...
- Intratumoral Injection of Clostridium novyi-NT Spo...
- Preclinical activity of SAR408701, a novel anti-CE...
- A cyclin D1-dependent transcriptional program pred...
- A Morphometric Study of Stylomastoid Foramen with ...
- The Longissimus Capitis Insertion as a Superficial...
- Advancement of PI3 Kinase Inhibitor Combination Th...
- Preoperative Embolization on Juvenile Nasopharynge...
- Management of Cutaneously Exposed Carotid Stents i...
- IntraOmmaya compartmental radioimmunotherapy using...
- Upper respiratory tract infection
- Behavioural Brain Research
- bioRxiv Subject Collection: Evolutionary Biology
- International Journal of Environmental Research ...
- Antioxidants
-
▼
Οκτ 12
(80)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
►
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
- ► Φεβρουαρίου (793)
Αναζήτηση αυτού του ιστολογίου
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
https://ift.tt/2MQ8Ai8
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.