Microglia/macrophages express alternative proangiogenic factors depending on granulocyte content in human glioblastoma

Microglia/macrophages express alternative proangiogenic factors depending on granulocyte content in human glioblastoma:

Abstract

Myeloid cells are an inherent part of the microenvironment of glioblastoma multiforme (GBM). There is growing evidence for their participation in mechanisms of tumor escape, especially in development of resistance following initially promising anti‐VEGF/VEGFR treatment. Thus, we sought to define the capability of myeloid cells to contribute to the expression of proangiogenic molecules in human GBM. We investigated GBM specimens in comparison to anaplastic astrocytoma (WHO grade III) and epilepsy patient samples freshly obtained from surgery. Flow cytometric analyses revealed two distinct CD11b⁺CD45⁺ cell populations in GBM tissues, which were identified as microglia/macrophages and granulocytes. Due to varied granulocyte influx, GBM samples were subdivided into groups with low (GBM‐lPMNL) and high numbers of granulocytes (GBM‐hPMNL), which were related to activation of the microglia/macrophage population. Microglia/macrophages of the GBM‐lPMNL group were similar to those of astrocytoma specimens, but those of GBM‐hPMNL tissues revealed an altered phenotype by expressing high levels of CD163, TIE2, HIF1α, VEGF, CXCL2 and CD13. While microglia/macrophages represented the main source of alternative proangiogenic factors, additionally granulocytes participated by production of IL8 and CD13. Moreover, microglia/macrophages of the GBM‐hPMNL specimens were highly associated with tumor blood vessels, accompanied by remodeling of the vascular structure. Our data emphasize that tumor‐infiltrating myeloid cells might play a crucial role for limited efficacy of anti‐angiogenic therapy bypassing VEGF mediated pathways through expression of alternative proangiogenic factors.

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