Objectives To determine the transcription profile of the mouse nucleus pulposus (NP) and annulus fibrosus (AF) with an unbiased method. Furthermore, pathophysiological relevance of selected genes was demonstrated in the mouse tail intervertebral disc (IVD) injury model. Design Paired normal mouse NP and AF tissue from C57BL/6j mice was examined by a polymerase chain reaction (PCR) array. Key gene expression in the normal and injured IVDs were confirmed by Real-Time PCR. Results Among the 84 genes studied, 63 were expressed higher in AF than in NP; only 4 genes were expressed higher in NP than in AF (n=4, p≤0.05). Real-time PCR confirmed that cadherin (cdh)-2 gene expression was higher in NP than in AF, and type I collagen (col1) gene expression was higher in the AF than in NP (n=8, p
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Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
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heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
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