Molecular characterization of multidrug-resistant Pseudomonas aeruginosa isolates in hospitals in Myanmar [Epidemiology and Surveillance]

The emergence of multidrug-resistant (MDR) Pseudomonas aeruginosa has become a serious worldwide medical problem. This study was designed to clarify the genetic and epidemiological properties of MDR P. aeruginosa strains isolated from hospitals in Myanmar. Forty-five MDR P. aeruginosa isolates obtained from different patients in seven hospitals in Myanmar were screened by the broth microdilution method. The whole genomes of the MDR isolates were sequenced by MiSeq (Illumina). Phylogenetic trees were constructed from single nucleotide polymorphism concatemers. Multilocus sequence types were deduced and drug resistance genes were identified. Of the 45 isolates, 38 harbored genes encoding carbapenemases, including DIM-1, IMP-1, NDM-1, VIM-2 and VIM-5; and nine had genes encoding 16S rRNA methylases, including RmtB, RmtD3, RmtE and RmtF2. Most strains of MDR P. aeruginosa isolated in Myanmar belonged to ST1047. This is the first molecular epidemiological analysis of MDR P. aeruginosa clinical isolates in Myanmar. These findings strongly suggest that P. aeruginosa ST1047 harboring carbapenemases, including DIM-, IMP-, NDM- and VIM-type metallo-β-lactamases, have been spreading throughout medical settings in Myanmar.

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