Exosome-Transmitted PSMA3 and PSMA3-AS1 Promotes Proteasome Inhibitors Resistance in Multiple Myeloma

Purpose: How exosomal RNAs released within the bone marrow microenvironment affect proteasome inhibitors (PIs) sensitivity of multiple myeloma (MM) is currently unknown. This study aims to evaluate which exosomal RNAs are involved and by which molecular mechanisms they exert this function. Experimental Design: Exosomes were characterized by dynamic light scattering, transmission electron microscopy and western blot. Coculture experiments were performed to assess exosomal RNAs transferring from mesenchymal stem cells (MSCs) to MM cells. The role of PSMA3-AS1 in PIs sensitivity was further evaluated in vivo. To determine the prognostic significance of circulating exosomal PSMA3 and PSMA3-AS1, a cohort of newly diagnosed MM patients was enrolled to study. Cox regression models and Kaplan-Meier curves were used to analyze progression free survival (PFS) and overall survival (OS). Results: We identified that PSMA3 and PSMA3-AS1 in MSCs could be packaged into exosomes and transferred to myeloma cells, thus promoted PIs resistance. PSMA3-AS1 could form an RNA duplex with pre-PSMA3, which transcriptionally promoted PSMA3 expression by increasing its stability. In xenograft models, intravenously administered siPSMA3-AS1, was found to be effective in increasing carfilzomib sensitivity. Moreover, plasma circulating exosomal PSMA3 and PSMA3-AS1 derived from MM patients were significantly associated with PFS and OS. Conclusion: This study suggested a unique role of exosomal PSMA3 and PSMA3-AS1 in transmitting PIs resistance from MSCs to MM cells, through a novel exosomal PSMA3-AS1/PSMA3 signaling pathway. Exosomal PSMA3 and PSMA3-AS1 might act as promising therapeutic targets for PIs resistance and prognostic predictors for clinical response.

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