In-vitro evaluation of biofilm dispersal as a therapeutic strategy to restore antimicrobial efficacy [PublishAheadOfPrint]

As a proof-of-concept study, the direct impact of biofilm dispersal on the in-vitro efficacy of imipenem and tobramycin was evaluated against three-day-old biofilms of Pseudomonas aeruginosa. Arabinose induction of biofilm dispersal via activation of the phosphodiesterase yhjH in P. aeruginosa engineering strain PAO1/p_BAD-yhjH resulted in an increased antimicrobial efficacy and synergy of the imipenem-tobramycin combination. These results support the use of biofilm dispersal to enhance antimicrobial efficacy in the treatment of biofilm-associated infections, representing a promising therapeutic strategy.

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