Gametocytes from K13-propeller mutant Plasmodium falciparum clinical isolates demonstrate reduced susceptibility to dihydroartemisinin in the male gamete exflagellation inhibition assay [Susceptibility]

Mutations in the kelch-propeller domain (K13-propeller) of Plasmodium falciparum parasites from Southeast Asia are associated with reduced susceptibility to artemisinin. We exposed in vitro-cultured Stage V gametocytes from Cambodian K13-propeller mutant parasites to dihydroartemisinin and evaluated inhibition of male gamete formation in the in vitro exflagellation inhibition assay (EIA). Gametocytes with R539T and C580Y K13-propeller alleles were less susceptible to dihydroartemisinin and had significantly higher 50% inhibitory concentrations (IC₅₀s) compared to gametocytes with wild-type alleles.

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