Publication date: Available online 13 February 2018
Source:The Spine Journal
Author(s): Li Yao, Nikol Flynn
Background ContextAdvances in the development of biomaterials and stem cell therapy provide a promising approach to regenerating degenerated discs. The normal nucleus pulposus (NP) cells exhibit the similar phenotype as chondrocytes. Because dental pulp stem cells (DPSCs) can be differentiated into chondrogenic cells, the DPSCs and DPSCs-derived chondrogenic cells encapsulated in type I and type II collagen hydrogels can potentially be transplanted into degenerated nucleus pulposus (NP) to repair damaged tissue. The motility of transplanted cells is critical because the cells need to migrate away from the hydrogels containing the cells of high density and disperse into the NP tissue after implantation.PurposeThe purpose of this study was to determine the motility of DPSC and DPSC-derived chondrogenic cells in type I and type II collagen hydrogels.Study Design/SettingThe time lapse imaging that recorded cell migration was analyzed to quantify the cell migration velocity and distance.MethodsThe cell viability of DPSCs in native or 4S-StarPEG – crosslinked type I and type II collagen hydrogels was determined using LIVE/DEAD® cell viability assay and AlamarBlue® assay. DPSCs were differentiated into chondrogenic cells. The migration of DPSCs and DPSC-derived chondrogenic cells in these hydrogels was recorded using a time lapse imaging system. This study was funded by Regional Institute on Aging and Wichita Medical Research and Education Foundation and the authors declare no competing interest.ResultDPSCs showed high cell viability in non-crosslinked and crosslinked collagen hydrogels. DPSCs migrated in collagen hydrogels, and the cell migration speed was not significantly different in either type I collagen or type II collagen hydrogels. The migration speed of DPSC-derived chondrogenic cells was higher in type I collagen hydrogel than in type II collagen hydrogel. Crosslinking of type I collagen with 4S-StarPEG significantly reduced the cell migration speed of DPSC-derived chondrogenic cells.ConclusionsAfter implantation of collagen hydrogels encapsulating DPSCs or DPSC-derived chondrogenic cells, the cells can potentially migrate from the hydrogels and migrate into the NP tissue. This study also explored the differential cell motility of DPSCs and DPSC-derived chondrogenic cells in these collagen hydrogels.
http://ift.tt/2BL4C7E
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
▼
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
-
▼
Φεβρουαρίου
(4835)
-
▼
Φεβ 22
(271)
- Establishment and characterization of in vivo orth...
- LncRNA HOTAIR influences cell growth, migration, i...
- Prognostic variables for temporal lobe injury afte...
- CGB5 expression is independently associated with p...
- Risk factors and survival outcomes in patients wit...
- Prediction of radiotherapy response with a 5-micro...
- Chromosomal abnormality variation detected by G-ba...
- Treatment-related mortality in newly diagnosed ped...
- Application of the red fluorescent protein mCherry...
- Variations in the relative abundance of Wolbachia ...
- The future of genomics in polar and alpine cyanoba...
- Arbuscular mycorrhizal fungal and soil microbial c...
- Lyme borreliosis–from tick bite to diagnosis and t...
- Epicrestal and subcrestal placement of platform-sw...
- A sustainable tandem catalysis approach to plant o...
- Employment benefits and job retention: evidence am...
- Central nervous system relapse in patients over 80...
- Natural occurrence and production of tenuazonic ac...
- Adult Wilms tumor with inferior vena cava thrombus...
- Prediction of suboptimal cytoreductive surgery in ...
- The extremity localized classic osteosarcomas have...
- Fast dictionary learning from incomplete data
- Immunomodulation of mesenchymal stem cells in disc...
- Prevalence and risk factors for neural axis anomal...
- Short-term outcomes following posterior cervical f...
- Back pain improves significantly following discect...
- Load-sharing through elastic micro-motion accelera...
- Risk factors for perioperative morbidity in spine ...
- Dental pulp stem cell-derived chondrogenic cells d...
- ‘Micro endoscopic’ vs. ‘pure endoscopic’ surgery f...
- Incidence and risk factors of post-operative neuro...
- Re: Are Modic changes associated with intervertebr...
- Risk-factor analysis of adjacent segment disease r...
- Risk factors & associated complications for postop...
- Motor impairment in patients with chronic neck pai...
- Impaction durability of porous PEEK and titanium-c...
- Do findings identified on magnetic resonance imagi...
- Ixazomib in the management of relapsed multiple my...
- Reporting guidelines for oncology research: helpin...
- EQUATOR-Oncology: reducing the latitude of cancer ...
- Mass spectrometry-based proteomics reveals potenti...
- Clinical Factors Predict Atezolizumab Response [Ne...
- Polyunsaturated fatty acids and risk of melanoma: ...
- Tumor-associated auto-antibodies as early detectio...
- Systematic Complex Haploinsufficiency-Based Geneti...
- Photosensitive Alternative Splicing of the Circadi...
- A Genome-Wide Association Study for Host Resistanc...
- EQUATOR-Oncology: reducing the latitude of cancer ...
- Reporting guidelines for oncology research: helpin...
- Progression of corticospinal tract dysfunction in ...
- Value of ictal and interictal epileptiform dischar...
- Adherence to colorectal cancer screening measured ...
- Identification of volumetric laser endomicroscopy ...
- Implementation of Medical Assistance in Dying (MAi...
- Demonstration of safety and feasibility of hydroge...
- A Phase II Study of Stereotactic Body Radiotherapy...
- Impact of Widespread Cervical Cancer Screening: Nu...
- Induction Therapies Plus Surgery Versus Exclusive ...
- Early Results From the Implementation of a Lung Ca...
- Is Hormone Replacement Therapy Safe in Women With ...
- Long-term Outcomes After Radiosurgery for Temporal...
- Primary Debulking Surgery Versus Neoadjuvant Chemo...
- Factors Associated With Receipt of Radiation Thera...
- Stage I and II Endometrial Adenocarcinoma: Analysi...
- A Phase 2 Randomized, Double-Blind, Multicenter Tr...
- Use of Radiation Therapy in Locally Advanced Pancr...
- The Benefit of Adjuvant Radiotherapy in High-grade...
- Albumin and Neutrophil-Lymphocyte Ratio (NLR) Pred...
- Breast Cancer is Common in Women With Ovarian Mali...
- Immunogenicity of Influenza Vaccination in Patient...
- Molecular Switch Links High-Fat Diet to Prostate C...
- Clarithromycin (Biaxin): Drug Safety Communication...
- Rapidity of Correction of Hyponatremia Due to Synd...
- Management of Acute Kidney Injury: Core Curriculum...
- Report of the Standardized Outcomes in Nephrology–...
- Numerical study on the hemodynamics of patient-spe...
- Comparing and optimizing ultraviolet germicidal ir...
- Clarithromycin (Biaxin): Drug Safety Communication...
- Four-pulse transcranial magnetic stimulation using...
- Visually Based Characterization of the Incipient P...
- A New Method for Inducing a Depression-Like Behavi...
- Effect of propranolol on heart rate variability in...
- Retrograde Neuroanatomical Tracing of Phrenic Moto...
- Organellar stress intersects the astrocyte endopla...
- Arginase-II activates mTORC1 through myosin-1b in ...
- Estradiol signaling mediates gender difference in ...
- Hints on ATGL implications in cancer: beyond bioen...
- Metformin exerts multitarget antileukemia activity...
- Role of autophagy in modulating post-maturation ag...
- PU.1/microRNA-142-3p targets ATG5/ATG16L1 to inact...
- Transient Expression of Foreign Genes in Insect Ce...
- Association between proton pump inhibitors use and...
- Electrophysiological Assessment of Murine Atria wi...
- The Abscopal Effect in the Era of Cancer Immunothe...
- CRISPR/Cas9-mediated modification of the extreme C...
- Pleiotropic effects of heparins: does anticoagulan...
- Dorsal root ganglia volume differentiate schwannom...
- Macrophage-Derived Extracellular Succinate License...
- Bis-2(5H)-furanone Derivatives as New Anticancer A...
- Biological Activities of 3,4,5-Trihydroxypiperidin...
-
▼
Φεβ 22
(271)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
►
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
- ► Φεβρουαρίου (793)
Αναζήτηση αυτού του ιστολογίου
Πέμπτη 22 Φεβρουαρίου 2018
Dental pulp stem cell-derived chondrogenic cells demonstrate differential cell motility in type I and type II collagen hydrogels
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
Alimentary Pharmacology &Therapeutics, EarlyView. https://ift.tt/2qECBIJ
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.