Gene Identification of Pheromone Gland Genes Involved in Type II Sex Pheromone Biosynthesis and Transportation in Female Tea Pest Ectropis grisescens

Moths can biosynthesize sex pheromones in the female sex pheromone glands (PGs) and can distinguish species-specific sex pheromones using their antennae. However, the biosynthesis and transportation mechanism for Type II sex pheromone components has rarely been documented in moths. In this study, we constructed a massive PG transcriptome database (14.72 giga bases) from a moth species, Ectropis grisescens, which uses Type II sex pheromones and is a major tea pest in China. We further identified the putative sex pheromone biosynthesis and transportation related genes: 111 cytochrome P450 monooxygenases (P450s, CYPs), 25 odorant binding proteins (OBPs), and 20 chemosensory proteins (CSPs). Tissue expression and phylogenetic tree analyses showed that one CYP (EgriCYP341-fragment3), one OBP (EgriOBP4), and one CSP (EgriCSP10) gene displayed an enriched expression in the PGs, and that EgriOBP2, 3, and 25 are clustered in the moth PBP clade. We considered these our candidate genes. Our results yielded large-scale PG sequence information for further functional studies.

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