RubisCO Early Oxygenase Activity: A Kinetic and Evolutionary Perspective

RubisCO (D-ribulose 1,5-bisphosphate carboxylase/oxygenase) is Earth's main enzyme responsible for CO₂ fixation via carboxylation of ribulose-1,5-bisphosphate (RuBP) into organic matter. Besides the carboxylation reaction, RubisCO also catalyzes the oxygenation of RuBP by O₂, which is probably as old as its carboxylation properties. Based on molecular phylogeny, the occurrence of the reactive oxygen species (ROS)-removing system and kinetic properties of different RubisCO forms, we postulated that RubisCO oxygenase activity appeared in local microoxic areas, yet before the appearance of oxygenic photosynthesis. Here, in reviewing the literature, we present a novel hypothesis: the RubisCO early oxygenase activity hypothesis. This hypothesis may be compared with the exaptation hypothesis, according to which latent RubisCO oxygenase properties emerged later during the oxygenation of the Earth's atmosphere. The reconstruction of ancestral RubisCO forms using ancestral sequence reconstruction (ASR) techniques, as a promising way for testing of RubisCO early oxygenase activity hypothesis, is presented.

RubisCO catalyzes carboxylation and oxygenation of ribulose-1,5-bisphosphate. Carboxylating properties of RubisCO are an ancient way for CO₂ fixation into organic matter. However, an evolutionary importance of oxygenase activity of RubisCO remains enigmatic. Here, we formulated RubisCO early oxygenase activity hypothesis. According to this hypothesis, RubisCO oxygenase activity appeared yet before the appearance of oxygenic photosynthesis.

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