Thalassiella azotovora gen. nov., sp. nov., a New Member of the Family Kineosporiaceae Isolated from Sea Water in South Korea

Abstract

A gram-positive, nonmotile, rod-shaped, nonflagellated, aerobic bacterium, designated strain DSD2^T was isolated from a seawater sample from Sadong wharf, Ulleung-Island, South Korea. Strain DSD2^T was found to be able to grow at pH ranging from 6 to 11 (optimum 7–8), 0–7 % (w/v) NaCl (optimum 0 %), at 10–42 °C (optimum 37 °C). 16S rRNA gene sequence analysis revealed that strain DSD2^T was 95.8 % similar to the type strain of Kineosporia rhamnosa KACC 15195^T, 95.8 % similar to Angustibacter aerolatus KACC 15527^T, and 95.5 % similar to Kineococcus xinjiangensis KCTC 19474^T as its closest relatives. A neighbor-joining phylogenetic tree based on the 16S rRNA gene sequence showed that strain DSD2^T related to Micrococcineae and Kineosporiineae suborder clade. The major polar lipids were phosphoglycolipids and phospholipids. Strain DSD2^T was found to contain MK-8 (H₂) and MK-9 (H₄) as the predominant menaquinone and iso-C_16:0 as the major fatty acid. The isolate contained meso-diaminopimelic acid (meso-A₂pm) with alanine, glutamic acid, and glycine as the diagnostic diamino acid. The DNA G+C content of strain DSD2^T was 73.2 mol%. On the basis of phylogenetic, biochemical, chemotaxonomic, and other physiological characteristics, strain DSD2^T is assigned to a novel species of a novel genus within the suborder Kineosporiineae and the name Thalassiella azotovora gen nov., sp. nov., is proposed. The type strain is DSD2^T (= KCTC 39634^T = JCM 31134^T).

http://ift.tt/2d5Xgyy