Oceanisphaera aquimarina sp. nov., Isolated from Oil-Contaminated Sediment of Ocean Coastal Area from South Korea

Abstract

Strain S33^T was isolated from oil-contaminated sediment of Tae-an coastal region of South Korea. Cells are aerobic, motile, Gram staining-negative, and coccoid shaped. Strain S33^T grew optimally at the temperature of 25 °C (range of 4–40 °C), pH 6.0 (range of pH 6.0–10.0), and in the presence of 1 % (w/v) NaCl (range of 0–10 %). Ubiquinone-8 was the predominant respiratory quinone. C_16:0, summed feature 3 (comprising C_16:1ω7c/C_16:1ω6c) and C_18:1ω7c were the major fatty acids. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. Strain S33^T showed the ability to degrade benzene, toluene, and ethylbenzene after 3 days incubation. 16S rRNA gene sequence analysis showed that the strain S33^T was most closely related to Oceanisphaera sediminis TW92^T (97.3 %), Oceanisphaera profunda SM1222^T (97.2 %), and Oceanisphaera ostreae T-w6^T (97.1 %) and <97 % with other members of the genus Oceanisphaera. The genomic DNA G+C mol% content of strain S33^T was 51.0 mol%. Based on distinct phenotypic, genotypic, and phylogenetic analysis, strain S33^T was proposed to represent a novel species in the genus Oceanisphaera as Oceanisphaera aquimarina sp. nov. (= KEMB 1002-058^T = JCM 30 794^T).

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