Development of a 450 nm laser irradiation desorption method for fast headspace solid‐phase microextraction of volatiles from krill oil (Euphausia superba)

Abstract

A laser irradiation desorption (LID) method was developed using a 450 nm blue semiconductor laser and gas chromatography mass spectrometry (GC/MS) for analyzing the flavor of Antarctic krill oil (Euphausia superba). The experimental parameters of LID were optimized by response surface methodology (RSM), and the optima were obtained as following, laser power (10 w), irradiation time (5 min), and sample volume (3 g). The results showed that a total of 41 volatile molecular species were detected, including 6 alcohols, 10 aldehydes, 5 ketones, 14 hydrocarbons, and 6 other compounds. Compared with the conventional heating assisted desorption technique, the LID could release volatile molecular species with higher abundance in a considerably reduced extraction time from 30 to 5 min. Finally, the proposed method was validated in terms of sensitivity, precision, linearity and recovery, which demonstrated its advantages with high efficient and simplified procedure.

Practical applications: A laser irradiation desorption (LID) method was developed using a 450 nm blue semiconductor laser for analyzing the flavor of Antarctic krill oil (Euphausia superba). Compared with the conventional heating assisted desorption technique, the LID could release more volatile molecular species with higher abundance in a considerably shorter extraction time, and the intensive laser power would not induce the autoxidation of polyunsaturated fatty acids. It showed the advantages of high efficient, and simplified procedure.

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