Introduction. Honey bee venom (HBV) has various biological activities such as the inhibitory effect on several types of cancer. Cisplatin is an old and potent drug to treat most of the cancers. Our aim in the present study was to determine antimutagenic and cytotoxic effects of HBV on mammary carcinoma, exclusively and in combination with cisplatin. Methods. In this study, 4T1 cell line was cultured in RPMI-1640 with 10% fetal bovine serum (FBS), at 37°C in humidified CO2 incubator. The cell viabilities were examined by the MTT assay. Also, HBV was screened for its antimutagenic activity via the Ames test. The results were assessed by SPSS software version 19 and one-way ANOVA method considering level of significance. Results. The results showed that 6 mg/ml of HBV, 20 μg/ml of cisplatin, and 6 mg/ml HBV with 10 μg/ml cisplatin could induce approximately 50% of 4T1 cell death. The concentration 7 mg/ml of HBV with of 62.76% inhibitory rate showed the highest antimutagenic activity in comparison with other treatment groups. Conclusions. The MTT assay demonstrated that HBV and cisplatin could cause cell death in a dose-dependent manner. The cytotoxic effect of cisplatin also promoted by HBV. Ames test outcomes indicated that HBV could act as a significant mutagenic agent. The antimutagenic activity of HBV was increased considerably in the presence of S9 mix. Therefore, our findings have revealed that HBV can enhance the cytotoxic effect of cisplatin drug and its cancer-preventing effects.
http://bit.ly/2Sh1LvK
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
▼
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
-
▼
Φεβρουαρίου
(5586)
-
▼
Φεβ 09
(260)
- Investigation of Key Genes and Pathways in Inhibit...
- Preemptive isolation and active surveillance in th...
- An update on the antimicrobial susceptibility and ...
- Clinical outcome of patients with Clostridium diff...
- Surgery is safe and effective when indicated in th...
- Evaluation of QuickFISH and maldi Sepsityper for i...
- Evaluation of the 15 and 24-loci MIRU-VNTR genotyp...
- High nuc DNA load in whole blood is associated wit...
- Pleural effusions induced by human herpesviruses i...
- Adding double carbapenem therapy to the armamentar...
- Foodborne botulism in Turkey, 1983 to 2017
- Reproducibility between two readout methods of a c...
- Hydrogen peroxide vapour treatment inactivates nor...
- Imipenem-resistant ceftazidime-susceptible Pseudom...
- Comparison of the diagnostic accuracy of markers o...
- Phylogenetic typing and virulence gene profile of ...
- Multicentric intrahepatic cholangiocellular carcin...
- Evaluation of the serum fructosamine concentration...
- Oxidative stress and toxicity effect of Echium amo...
- Combined effects of dietary bovine lactoferrin, La...
- Effect of Crotalaria lachnosema Stapf . (Fabaceae)...
- The effects of different doses of onion ( Allium c...
- Light and scanning electron microscopic study of t...
- Medical management of conjunctivitis and shell rot...
- Vitamin D supplementation enhanced oral glucose to...
- Antioxidant effects of Allium cepa and cinnamon on...
- Impairment of neuro-renal cells on exposure to cos...
- Health status of indigenous and exotic chickens so...
- Students Introduce the 2019 Marquette Challenge
- "J BUON"[jour]; +24 new citations
- #APTACSM: Education, Inspiration, Community
- The White House PT
- Entrepreneurship in Physical Therapy
- How To Know If A Residency Is For You
- APTA’s 2019-2020 Public Policy Priorities
- People With Parkinson Disease Keep Moving Through ...
- Getting Involved as a Student, You Won't Regret It
- PT Licensure Compact: What Students Need To Know
- Student Leaders On Involvement and Engagement
- Anxiety Diaries My Whole Body is a Nervous System
- Changes to Payment for PTs
- APTA Releases a New 3-Year Strategic Plan
- Cadmium in Groundwater Consumed in the Rural Areas...
- Genomic Characterization of the Zinc Transcription...
- Dietary Intake of Cadmium, Chromium, Copper, Nicke...
- Selenium Levels in Community Dwellers with Type 2 ...
- Cadmium Exposure and Blood Telomere Length in Fema...
- Is the Cooking Salt Safe in China? Assessment of C...
- High-Protein Diet Ameliorates Arsenic-Induced Oxid...
- Glutathione Might Attenuate Cadmium-Induced Liver ...
- Lanthanum Chloride Impairs the Blood-Brain Barrier...
- Smoking Is Positively Associated with Antithyroper...
- The Effect of Maternal Supplementation of Zinc, Se...
- Calcium Channels, Rho-Kinase, Protein Kinase-C, an...
- Mineral and Trace Metal Concentrations in Seaweeds...
- Borax Supplementation Alleviates Hematotoxicity an...
- Association of Endothelial Function with Parental ...
- Effect of Participation with Accompanying Househol...
- Telemedicine and Cardiology in the Elderly in Fran...
- Analysis of In Vitro Osteoblast Culture on Scaffol...
- Antibacterial Activity of Coumarins and Carbazole ...
- Novelty of Bioengineered Iron Nanoparticles in Nan...
- Effect of “DXB-2030,” a Polyherbal Formulation, on...
- Allergic Airway-Induced Hypersensitivity Is Attenu...
- Role of Nonsteroidal Anti-Inflammatory Drugs (NSAI...
- Antimutagenic and Synergistic Cytotoxic Effect of ...
- Disease-Modifying Effects of Long-Term and Continu...
- Antipathogenic Potential of a Polyherbal Wound-Car...
- Nutraceuticals Inspiring the Current Therapy for L...
- Vaccination Controversies: An Adult Case of Post-V...
- Obtaining Informed Consent for Anesthesia in Elect...
- Thank You, Reviewers of Acta Médica Portuguesa
- Multimorbidity and Disease Severity Measured by th...
- Health in Europe and in Portugal – A Commentary on...
- Schizophrenia: What Non-Psychiatrist Physicians Ne...
- Medical Student Secrecy, its Link to the Duty of C...
- My Diabetes My Way: supporting online diabetes sel...
- Evaluation of some neuropharmacological effects of...
- A possible biomarker of neurocytolysis in infantil...
- Oxidative stress among L-2-hydroxyglutaric aciduri...
- Role of PUMA in the methamphetamine-induced migrat...
- Protective effects of Nesfatin-1 peptide on cerebr...
- Neurochemical alterations following the exposure t...
- Can a hypercholesterolemic diet change the basal b...
- The metabolic alterations within the normal appear...
- Intracerebroventricular injection of resveratrol a...
- Diagnosis of covert hepatic encephalopathy: a mult...
- Determination of Minimal Residual Disease in Multi...
- Industry’s Giant Leap Into Cellular Therapy: Catal...
- Immunotherapeutic Approaches for Multiple Myeloma:...
- Should Overall Survival Remain an Endpoint for Mul...
- Treatment-Free Remission in CML: the US Perspective
- Graft Versus Host Disease Clinical Trials: Is it T...
- Communication involving special populations: older...
- The role of splanchnic congestion and the intestin...
- Communication in cancer: its impact on the experie...
- Cancer and fertility: optimizing communication bet...
- Best practices on team communication: interprofess...
- Oncologists communicating with patients about assi...
- Culture, treatment paradigms, and advanced heart f...
-
▼
Φεβ 09
(260)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
►
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
- ► Φεβρουαρίου (793)
Αναζήτηση αυτού του ιστολογίου
Σάββατο 9 Φεβρουαρίου 2019
Antimutagenic and Synergistic Cytotoxic Effect of Cisplatin and Honey Bee Venom on 4T1 Invasive Mammary Carcinoma Cell Line
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
https://ift.tt/2MQ8Ai8
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.