The potential of hypericin and hyperforin for antiadhesion therapy to prevent metastasis of parental and oxaliplatin-resistant human adenocarcinoma cells (HT-29)

Cancer cells disseminate to other parts of the body during metastasis through the process of intravasation. The hypericin and hyperforin effect has been described to understand the signal mechanisms that stimulate or stunt cancer cell sprouting to metastasis on colon adenocarcinoma cells HT-29 and its resistant form HT-29-OxR. We focused on the key points of adhesion proteins (cadherin, integrin, selectin and syndecan) and also proteins participating in or contributing to the process of cancer cell migration and adhesion through genes expression and proteins levels. Treatment effects were identified as a consequence of decreased cell adhesion, changes of expression in the adhesive proteins as well as basal membrane degradation associated with changes in the expression of matrix proteinases and in their activity. Finally, the cells affected by hypericin or hyperforin were evaluated by monitoring the cancer cell adhesion properties and proliferation processes. Supplementary Fig. (Supplemental digital content 1, https://ift.tt/2mYN67v). Correspondence to Martina Šemeláková, PhD, Department of Cellular Biology, Institute of Biology and Ecology, Faculty of Science, P.J. Šafárik University in Košice, Srobarova 2, 041 54 Košice, Slovakia Tel: +421 556 222 1190; fax: +421 5562 22124; e-mail: martina.semelakova@upjs.sk Received June 4, 2018 Accepted July 4, 2018 Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved.

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