Background. This research assessed the in vitro antidiabetic activity and phytochemical constituents of the traditionally used medicinal plants, Psiadia punctulata and Meriandra bengalensis. Method. The leaves of both plants were subjected to cold extraction method using 70% ethanol and hot Soxhlet extraction using n-hexane, chloroform, methanol, and distilled water. The extracts were studied for their effect on glucose transport across yeast cells and inhibition of α-amylase and α-glucosidase enzyme activities. Thin-layer chromatographic analysis of ethanol extract was also undertaken. Results. The results of yeast glucose uptake assay revealed that extracts from both plants had a maximum increase in glucose uptake at the 25mM glucose concentration with a maximum dose of 2000μg/ml plant extract. The ethanol extract of P. punctulata and aqueous extract of M. bengalensis showed a high activity of 68% and 96%, respectively, at 25mM and 2000μg/ml of glucose and extract concentration. P. punctulata exerted peak inhibition activity of α-amylase of 37.5 ± 3% mg/dl (IC50 = 0.523 mg/dl) for methanol and distilled water extract at 0.5 mg/dl, respectively. M. bengalensis methanol extract exhibited the highest inhibition activity of 38 ± 8 % mg/dl (IC50 = 0.543 mg/dl) at 0.5 mg/dl. In the α-glucosidase inhibition assay, the methanolic extract of P. punctulata exhibited the highest inhibitory activity of 17.29 ± 9% mg/dl (IC50 = 0.761 mg/dl) at 0.5mg/dl. The chloroform extract of M. bengalensis had the highest inhibitory activity of 30 ± 5% mg/dl (mg/dl) at 0.5 mg/dL. Phytochemical analysis of the different extracts of P. punctulata and M. bengalensis revealed the presence of flavonoids, alkaloids, tannins, saponins, phytosterols, and carbohydrates. Thin-layer chromatography analysis of ethanolic extract of both plants indicated presence of 15 and 17 spots for P. punctulata and M. bengalensis respectively. Conclusion. P. punctulata and M. bengalensis extracts have moderate inhibitory activity against pancreatic α-amylase and relatively low inhibitory activities against α-glucosidase. The observed effects may be associated with the presence of flavonoids, saponins, and alkaloids. Additional in vivo analysis, toxicological studies, isolation, and structural characterization of the phytomolecules identified in this study and molecular docking studies should be undertaken.
https://ift.tt/2LkILGc
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
▼
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
-
▼
Ιουλίου
(5782)
-
▼
Ιουλ 16
(222)
- Overadjustment Bias as a Threat in the Drive for G...
- The Safety and Efficacy of Laparoscopic Common Bil...
- Mendelian Randomization: Progressing towards under...
- Mendelian randomization study shows no causal rela...
- Plasma urate and risk of Parkinson's disease: a Me...
- Notch3ECD immunotherapy improves cerebrovascular r...
- Identifying therapeutic targets from spontaneous b...
- The clinical significance of PINX1 expression in p...
- Prevalence of and Risk Factors for Colorectal Neop...
- Cryotherapy relieves pain and edema following ingu...
- Quality of communication and trust in patients wit...
- Terminally Ill Cancer Patients’ Concordance betwee...
- Cultural factors influencing Advance Care Planning...
- Taste alterations during treatment with protein ki...
- Advance care planning with patients who have end-s...
- Evaluation of N-Succinimidyl S-Acetylthioacetate L...
- Point-of-care umbilical arterial lactate and newbo...
- Consistency of Integrated Management of Newborn an...
- Cranial neural tube defect after trimethoprim expo...
- Knowledge is power: general practitioners prescrib...
- Imprint cytology: a useful screening test for diag...
- Maternal Proteins That Are Phosphoregulated upon E...
- Overexpression of eis without a mutation in promot...
- The Dengue virus in Nepal: gaps in diagnosis and s...
- IFNL4-{Delta}G allele is associated with an interf...
- Metformin Inhibits Prostate Cancer Progression by ...
- Evolutionary expression of HER2 conferred by chrom...
- Phase 1 Study of Seviteronel, a Selective CYP17 Ly...
- The splicing factor PTBP1 promotes expression of o...
- Activating transcription factor 4 modulates TGF{be...
- Deficits in mesolimbic reward pathway underlie soc...
- Effective diagnosis of persistent facial pain will...
- CD38-mediated immunosuppression as a mechanism of ...
- CARs versus BiTEs: A Comparison between T Cell-Red...
- Microdialysis Study of Aztreonam-Avibactam Distrib...
- Validation of Heelstick Microsampling to Optimize ...
- Extended Duration MK-8591-Eluting Implant as a Can...
- Effect of the synthetic bile salt analog CamSA on ...
- In vitro antiviral activity of cabotegravir agains...
- Characterization of the AmpC {beta}-lactamase from...
- Novel potent capsid assembly modulators regulate m...
- Increased Doses Lead to Higher Drug Exposures of L...
- Evaluation of Oritavancin in Combination with Rifa...
- Screening of preselected libraries targeting Mycob...
- Validation of a dried blood spot ceftriaxone assay...
- Significantly improved pharmacokinetics enhances i...
- Clinical Outcomes of the Oral Suspension versus De...
- Broad spectrum and safety of oral treatment with a...
- In silico typing and comparative genomic analysis ...
- Comprehensive substrate characterization of 22 ant...
- Impact of Antibiotic Treatment on the Burden of Na...
- Impact of an antifungal stewardship programme in a...
- Effective treatment of the Mycobacterium avium sub...
- In vitro minimal inhibitory concentrations of rifa...
- E-cigarette adverts and childrens perceptions of t...
- Antioxidant extract counteracts the effects of agi...
- Effects of hypothermia on lung inflammation in a r...
- Interleukin-4 protects from chemotherapy-induced p...
- The therapeutic effects of curcumin and capsaicin ...
- An experimental study on the preventive effects of...
- Synergistic effect of honey and Nigella sativa on ...
- Cardioprotective effect of lipstatin derivative or...
- A curcumin derivative J147 ameliorates diabetic pe...
- Impact of 1,25(OH) 2 D 3 on TG content in liver of...
- Development of a model for laparoscopic cholecyste...
- Research Progresses in Cancer Stem Cells of Three ...
- Occult blood in faeces is associated with all-caus...
- Occult blood in faeces: a window into health beyon...
- Dendriform pulmonary ossification leading to bilat...
- Immunohistochemistry for PAX7 is a useful confirma...
- Curcumin derivative, 2,6-bis(2-fluorobenzylidene)c...
- Animal-level risk factors associated with foot-and...
- Morning Glory Disc Anomaly in a Child with Esotropia
- A Girl with Delayed Puberty and Bumpy Lips
- Data Sharing Statements for Clinical Trials: One S...
- Harms Reporting in Randomized Controlled Trials of...
- Zika in 2018: Advising Travelers Amid Changing Inc...
- Early Experiences With Journal Data Sharing Polici...
- Making the Voices of Female Trainees Heard
- Relationships between tumour response and primary ...
- Delayed intra-articular pseudoaneurysm of the dors...
- Review: In type 2 diabetes, SGLT-2 inhibitors or G...
- Review: After PCI in AF, OAC plus single vs dual a...
- An Innovative Program to Support Gender Equity and...
- Clinicians' Perspectives on Providing Emergency-On...
- Annals Story Slam - Clerks, Codes, and Culture, or...
- Association of Viral Suppression With Lower AIDS-D...
- Review: In uncontrolled, persistent asthma, adding...
- Associations Between American Board of Internal Me...
- Annals for Hospitalists - 17 July 2018
- Marijuana Use, Respiratory Symptoms, and Pulmonary...
- Annals Story Slam - Seeing the Body, or Two Sparks
- Clonal Hematopoiesis Confers Predisposition to Bot...
- A Novel Strategy for Increasing Access to Treatmen...
- Review: More-intensive vs less-intensive LDL-chole...
- Starving the Hungry Tapeworm: How Businessmen May ...
- Annals Consult Guys – A Stumper: How Much Spent fo...
- Dialysis Without Borders
- Annals Story Slam - A Time to Die
- Maintenance of Certification: Glass Not Entirely E...
-
▼
Ιουλ 16
(222)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
►
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
- ► Φεβρουαρίου (793)
Αναζήτηση αυτού του ιστολογίου
Δευτέρα 16 Ιουλίου 2018
In Vitro Inhibition of -Amylase and -Glucosidase by Extracts from Psiadia punctulata and Meriandra bengalensis
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
https://ift.tt/2MQ8Ai8
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.