MiR-134 expression and changes in inflammatory cytokines of rats with epileptic seizures

OBJECTIVE: To investigate the expression of miR-134 and the change of inflammatory cytokines in seizure rats and to explore its relationship.

MATERIALS AND METHODS: A rat model of seizures was made by an intraperitoneal injection with kainic acid. ELISA kit for detection of seizures in rats was used. The changes of inflammatory cytokines (IL-1, IL-2, IL-6, TNF-α, IFN-γ) and the hippocampal neuronal cell growth were observed. The expression of miR-134 in brain tissue and serum samples of model group and control group was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) quantitative determination.

RESULTS: After the intraperitoneal injection of kainic acid, the rat model of seizure was successfully established. Compared with the control group, the neuronal cells in the hippocampus of the model group showed evident pathological changes. Inflammatory cytokines in seizures rats showed that the increase of IL-2, IL-6, and TNF-α were larger, but the increase of IL-1 and IFN-γ was not obvious. The results of the RT-PCR quantitative analysis showed that the expression of miR-134 in brain tissue and serum of epilepsy rats was lower than that of the control group (p<0.05).

CONCLUSIONS: The expression of miR-134 would be down by the increasing of inflammatory cytokines in the epileptic seizure.

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