Methicillin-resistant Staphylococcus aureus isolates lacking mec-genes (n=32) collected from Belgian hospitals were characterized for their β-lactamase production and the presence of mutations in pbp-genes, the pbp4-promotor and genes (gdpP, yjbH) involved in PBP4-overproduction. Twelve isolates were β-lactamase hyperproducers (BHP), while 12 non-BHPs isolates may produce an incomplete GdpP protein. Most isolates showed nucleotide missense mutations at pbp genes. Few isolates showed also mutations at the pbp4-promoter.
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