Biomolecules often undergo conformational changes, which are the driving mechanism for their molecular function. As discussed in article 1700078 by Voith von Voithenberg and Lamb, these intramolecular changes and their dynamics can be investigated using single pair Förster resonance energy transfer (spFRET). Confocal and total internal reflection fluorescence microscopy used for spFRET experiments can extract kinetic timescales of the dynamics in the range of nanoseconds to seconds. (PDB: 2YH0, 2YH1, 2KHO, 4B9Q, 1RP3, 1IW7)
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