Objective. To investigate the modulated effects of HRF on cyclooxygenase isoform expression and its activity, using the human umbilical vein endothelial cell (HUVEC) model induced by interleukin-1 beta (IL-1β). Methods. Cells were treated with indomethacin (positive control), HRF, and its components at various concentrations prior to treatment with IL-1β at 24 h. Cell viability was determined by MTT assay. Moreover, the anti-inflammatory effects of HRF and its components through mRNA and protein expression were established using real-time quantitative PCR and Western blot, respectively. COX activity was identified via exogenous and endogenous PGE2 productions using the EIA. Result. There was no cytotoxicity in HUVECs treated with HRF. None of the experimental conditions used in the study affected the expression of COX-1, but COX-2 protein expression was inhibited at concentrations under 10 µg/mL. Despite the significantly increased levels of exogenous PGE2, HRF had no effect on COX-2 mRNA expression. However, the production of PGE2 was lower at a concentration of 100 µg/mL HRF than at a concentration below 10 µg/mL. Interestingly, each component of HRF revealed different effects of the Ha-Rak formula. Conclusion. Our preliminary findings suggest that HRF and its components provide diverse modulation of COX-2 and PGE2 at the in vitro level.
http://ift.tt/2zf9HEK
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
▼
2017
(44259)
- ► Δεκεμβρίου (5110)
-
▼
Οκτωβρίου
(5141)
-
▼
Οκτ 29
(47)
- Immediately restored full arch-fixed prosthesis on...
- People Who Value Virtue Show Wiser Reasoning
- Brain signal intensity changes as biomarkers in am...
- Combining olfactory test and motion analysis senso...
- The death of Ben Hall
- Screening of Natural Bioactive Metabolites and Inv...
- Chinese Herbal Medicine as an Adjunctive Therapy A...
- Safety Assessment and Comparison of Sodium Selenit...
- The Roles of Thrombospondins in Hemorrhagic Stroke
- miRNAs: Important Targets for Oral Cancer Pain Res...
- EMCrit Podcast 211 – Expertise with Anders Ericsson
- Use of Synaptic Zinc Histochemistry to Reveal Diff...
- How Well Can Modern Nonhabitual Barefoot Youth Ada...
- NLRP1 Overexpression Is Correlated with the Tumori...
- Protective Effects of Pyridoxamine Supplementation...
- Current Tissue Molecular Markers in Colorectal Can...
- Scorpion Venom Active Polypeptide May Be a New Ext...
- Systems Pharmacological Approach to the Effect of ...
- Reversal of Proximal Renal Tubular Dysfunction aft...
- Invasive Nontyphoidal Salmonella Infection in a Pa...
- Glycolipid-peptide conjugate vaccines enhance CD8(...
- γδ T Cells Are Required for the Induction of Steri...
- Immunization against full-length protein and pepti...
- Antimicrobial Activities and Time-Kill Kinetics of...
- The Effects of Thai Herbal Ha-Rak Formula on COX I...
- Complications of Trans Arterial Embolization Durin...
- What Healthcare Workers Should Know about Environm...
- Effect of Sheng-Jiang Powder on Obesity-Induced Mu...
- Feasibility of Round Window Stimulation by a Novel...
- Fabrication and In Vitro Study of Tissue-Engineere...
- Duplex ultrasound in the early diagnosis of acute ...
- Mortality in Spanish pediatric emergency departmen...
- Strengths and weaknesses in team communication pro...
- Prehospital management and outcome of avalanche pa...
- Does it matter who places the intravenous? An inte...
- Intermediate-term and long-term mortality among ac...
- Implementing an electronic observation and early w...
- Improved detection of delirium, implementation and...
- What will emergency care look like 10 years from now?
- The Abbreviated Mental Test 4 for cognitive screen...
- Intranasal fentanyl for the prehospital management...
- How do patients with chest pain access Emergency D...
- Adolescent tracheal intubation in an adult urban e...
- Prognosis of patients with syncope seen in the eme...
- Contamination of urinary cultures in initial-strea...
- Characteristics and outcome of patients presenting...
- Top Ten Abstracts of the Tenth European Congress o...
-
▼
Οκτ 29
(47)
- ► Σεπτεμβρίου (5105)
-
►
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
- ► Φεβρουαρίου (793)
Αναζήτηση αυτού του ιστολογίου
Κυριακή 29 Οκτωβρίου 2017
The Effects of Thai Herbal Ha-Rak Formula on COX Isoform Expression in Human Umbilical Vein Endothelial Cells Induced by IL-1β
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
https://ift.tt/2MQ8Ai8
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.