Telocytes in the atherosclerotic carotid artery: Immunofluorescence and TEM evidence

xlomafota13 shared this article with you from Inoreader Telocytes in the atherosclerotic carotid artery: Immunofluorescence and TEM evidence Via histochem Acta Histochem. 2021 Feb;123(2):151681. doi: 10.1016/j.acthis.2021.151681. Epub 2021 Jan 22. ABSTRACT Telocytes, which possess distinct body shapes and long telopodes, are allocated in the vascular wall. As a fundamental cell type, telocytes construct a three-dimensional network to form a support structure for the artery. This study aims to characterize the morphology and ultrastructure of telocytes in atherosclerotic arteries. ApoE gene-deficient mice were selected as the a therosclerosis animal model and fed a high-fat diet for at least 12 weeks, and immunofluorescence assays and transmission electron microscopy techniques were used to observe changes in telocytes in atherosclerotic arteries. By immunofluorescence staining, CD34, CD117 and PDGFR-α were positive compared with negative CD28/vimentin in telocytes in the atherosclerotic carotid artery, and they were distributed in the tunica intima and tunica adventitia. Under transmission electron microscopy, the bodies of telocytes became larger, while telopodes became shorter compared with their normal condition, and a mass of lipidosomes was present during the progression of atherosclerosis. These results demonstrate that immunofluorescence with TEM is the critical method for identifying TCs and that steatosis of TCs is a reason for atherosclerotic artery dysfunction. PMID:33493960 | DOI:10.1016/j.acthis.2021.151681 View on the web Inoreader. Take back control of your news feed. Follow us on Twitter and Facebook.