SNARE-mediated fusion of single chromaffin granules with pore-spanning membranes

Pore-spanning membranes (PSMs) composed of supported membrane parts (s-PSMs) as well as freestanding membrane parts (f-PSMs) are shown to be very versatile to investigate SNARE-mediated fusion on the single particle level. They provide a planar geometry readily accessible by confocal fluorescence microscopy, which enabled us for the first time to investigate the fusion of individual natural secretory granules – chromaffin granules (CGs) – on the single particle level by two color fluorescence microscopy in a time-resolved manner.

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