Diversity and metabolism of xylose and glucose fermenting microbial communities in sequencing batch or continuous culturing

Abstract

A mechanistic understanding of microbial community establishment and product formation in open fermentative systems can aid the development of bioprocesses utilising organic waste. Kinetically, a single rate-limiting substrate is expected to result in one dominant species. Four enrichment cultures were operated to ferment either xylose or glucose in a sequencing batch reactor (SBR) or a continuous-flow stirred tank reactor (CSTR) mode. The combination of 16S rRNA gene-based analysis and fluorescence in situ hybridization revealed no complete dominance of one species in the community. The glucose-fed and xylose-fed SBR enrichments were dominated >80% by one species. Enterobacteriaceae dominated the SBRs enrichments, with Citrobacter freundii dominant for xylose and Enterobacter cloacae for glucose. Clostridium, Enterobacteriaceae and Lachnospiraceae affiliates dominated the CSTRs enrichments. Independent of substrate, SBR communities displayed 2–3 times higher biomass specific rate of substrate uptake (q_s^max) and 50% lower biomass yield on ATP, to CSTR communities. Butyrate production was linked to dominance of Clostridium and low q_s^max (1.06 Cmol_s Cmol_x⁻¹ h⁻¹), while acetate and ethanol production was linked to dominance of Enterobacteriaceae and Lachnospiraceae and high q_s^max (1.72 Cmol_s Cmol_x⁻¹ h⁻¹ and higher). Overall, more diversity than expected through competition was observed, indicating mutualistic mechanisms might shape microbial diversity.

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