Automatic Detection and Classification of Ca2+ Release Events in Confocal Line- and Frame-scan Images

Analysis of Ca2+ signals obtained in various cell types (i.e. cardiomyocytes) is always a trade-off between acquisition speed and signal-to-noise ratio of the fluorescence signal. This becomes especially apparent during fast two- or three-dimensional confocal imaging when local intracellular fluorescence signals originating from Ca2+ release from intracellular Ca2+ stores (e.g. sarcoplasmic reticulum, SR) need to be examined. Mathematical methods have been developed to remedy a high noise level by fitting each pixel with a transient function to "denoise" the image.

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