Diversity of endophytic fungi of Paeonia lactiflora Pallas and screening for fungal paeoniflorin producers

Abstract

Endophytic fungi from Paeonia lactiflora Pallas, which is mainly distributed in China, were characterized and screened to identify those capable of producing paeoniflorin. A total of 101 isolates obtained from the roots, stems, and leaves of P. lactiflora. were grouped into 16 fungal taxa based on morphological traits and ITS sequences, indicating that endophytic fungi of P. lactiflora are abundant and diverse. The dominant endophytic fungi were Aspergillus, Alternaria, and Penicillium. Fungi recovered from leaves were more than roots and stems. The similarity index was highest between the stems and leaves (0.733), followed by the roots and leaves (0.615) and the stems and roots (0.563). Analyses of the fermentation extracts of 22 endophytic fungi by HPLC and Mass spectrometry revealed that three strains (R12, Alternaria tenuissima; S4, Aspergillus flavus, and R17 Penicillium commune) were able to produce paeoniflorin. Among the paeoniflorin-producing fungi, the yield of paeoniflorin from Aspergillus flavus S4 was 342.4 μg/L. And this strain could be used as candidates for the industrial production of paeoniflorin.

https://ift.tt/2RTRwtB