Reduced CD160 expression contributes to impaired NK cell function and poor clinical outcomes in patients with HCC

We previously reported that deficiencies in NK cell number and function play an important role in the progression of hepatocellular carcinoma (HCC). However, the mechanisms underlying this phenomenon remain obscure. In this study, we analyzed the expression of CD160 on intrahepatic NK cells by evaluating peritumoral and intratumoral tissues of 279 HCC patients and 20 healthy livers. We observed reduced expression of CD160 on intratumoral NK cells, and patients with lower CD160 cell densities within tumor cells exhibited worse disease and a higher recurrence rate. High-resolution microarray and gene set enrichment analysis (GSEA) of flow cytometry-sorted primary intrahepatic CD160+ and CD160- NK cells of healthy livers indicated that human CD160+ NK cells exhibited functional activation, high IFN-γ production, and NK-mediated immunity. In addition, global transcriptomic analysis of sorted peritumoral and intratumoral CD160+ NK cells revealed that intratumoral CD160+ NK cells are more exhausted than peritumoral CD160+ NK cells and produce less IFN-γ. High levels of TGF-β1 interfered with production of IFN-γ by CD160+ NK cells, blocking of which specifically restored IFN-γ production in CD160+ NK cells to normal levels. These findings indicate that reduced numbers of CD160+ NK cells, together with the functional impairment of CD160+ NK cells by TGF-β1, contribute to tumor immune escape. Additionally, restoring the expression of CD160 and blocking TGF-β1 appears a promising therapeutic strategy against liver cancer.

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