Synthetic Transient Crosslinks Program the Mechanics of Soft, Biopolymer-Based Materials

Abstract

Actin networks are adaptive materials enabling dynamic and static functions of living cells. A central element for tuning their underlying structural and mechanical properties is the ability to reversibly connect, i.e., transiently crosslink, filaments within the networks. Natural crosslinkers, however, vary across many parameters. Therefore, systematically studying the impact of their fundamental properties like size and binding strength is unfeasible since their structural parameters cannot be independently tuned. Herein, this problem is circumvented by employing a modular strategy to construct purely synthetic actin crosslinkers from DNA and peptides. These crosslinkers mimic both intuitive and noncanonical mechanical properties of their natural counterparts. By isolating binding affinity as the primary control parameter, effects on structural and dynamic behaviors of actin networks are characterized. A concentration-dependent triphasic behavior arises from both strong and weak crosslinkers due to emergent structural polymorphism. Beyond a certain threshold, strong binding leads to a nonmonotonic elastic pulse, which is a consequence of self-destruction of the mechanical structure of the underlying network. The modular design also facilitates an orthogonal regulatory mechanism based on enzymatic cleaving. This approach can be used to guide the rational design of further biomimetic components for programmable modulation of the properties of biomaterials and cells.

Biomimetic crosslinking constructs for actin networks are prepared from synthetic materials, by chemically conjugating polypeptide-based binding domains onto double-stranded DNA scaffolds. The resulting biohybrid networks display mechanical and morphological features remarkably similar to their biologically derived counterparts. The DNA-based design enables a modular approach, where structural parameters and functional features, such as an enzymatic regulatory mechanism, can be directly integrated.

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