Antitumor activity of antibody against cytotoxic T lymphocyte epitope peptide of lymphocyte-specific protein tyrosine kinase

Abstract

Although humoral responses against cytotoxic T lymphocyte epitope peptides from lymphocyte-specific protein tyrosine kinase antigen have been observed in the majority of healthy donors and cancer patients, the biological activity of the antibody has not been determined. Then we investigated the biological activity of monoclonal antibody against cytotoxic T lymphocyte epitope peptide of lymphocyte-specific protein tyrosine kinase antigen at positions 486–494 (anti-lymphocyte-specific protein tyrosine kinase-486 monoclonal antibody). This monoclonal antibody induced dendritic cell maturation from murine bone marrow cells by the immune complex form in vitro, and inhibited tumor growth in association with a suppression of tumor infiltrating T cells, including T regulatory cells in a murine model using the female BALB/cCrlCrlj mice (H-2K^d). More potent tumor inhibition was observed when this monoclonal antibody was administered prior to the peptide vaccination. These results may help to unveil the biological activity of anti-lymphocyte-specific protein tyrosine kinase peptide antibodies against cytotoxic T lymphocyte epitope peptides.

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