Inside cover story: Eur. J. Immunol. 1'18

Our inside cover features an immunofluorescence staining of human T cells in contact with lymphoblastoid B cells, which naturally express the IL4I1 enzyme. The B cells were seeded onto polylysine treated glass coverslips before adding allogeneic T cells labeled with a fluorescent dye (red). The conjugates were next permeabilized and labeled with an anti-IL4I1 antibody revealed by a fluorescent secondary antibody (green). In the image, taken from Aubatin et al. (pp. 106–119), IL4I1 granules secreted by B cells into the immune synaptic cleft, induce global T-cell inhibition by decreasing the early signaling events downstream of TCR stimulation.

http://ift.tt/2CLq1eM