Cytocompatibility of Biodentine and iRoot FS with human periodontal ligament cells: an in vitro study

Abstract

Aim

To evaluate the cytocompatibility of Biodentine and iRoot FS with human periodontal ligament cells (hPDLCs).

Methodology

hPDLCs were characterized by flow cytometry and immunocytochemical analysis. hPDLC adhesion was assessed by scanning electron microscopy at day 3; proliferation by live/dead assay at day 1, 3 and 7; and osteogenic differentiation by alkaline phosphatase (ALP) activity staining, ALP quantification analysis and qRT-PCR at day 7 and 14. Data were analysed with ANOVA and independent sample t-tests with SPSS 21.0.

Results

Both iRoot FS and Biodentine increased the adhesion of hPDLCs at day 3. Compared to Biodentine, iRoot FS positively increased hPDLC proliferation on day 3 (P=0.03) and 7 (P=0.00). Osteogenic marker ALP was observed consistently in all samples, with iRoot FS showing significantly higher ALP activity at day 14 (P=0.00). Compared with Biodentine, iRoot FS significantly increased the mRNA level of ALP, COL1 and Runx2, and OCN increased only on day 14 (P< 0.05).

Conclusions

iRoot FS had a positive effect on the adhesion, proliferation and biomineralization of hPDLCs compared with Biodentine.

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