Antiamylase, Anticholinesterases, Antiglycation, and Glycation Reversing Potential of Bark and Leaf of Ceylon Cinnamon (Cinnamomum zeylanicum Blume) In Vitro

Ethanol (95%) and dichloromethane : methanol (DCM : M, 1 : 1 v/v) bark extracts (BEs) and leaf extracts (LEs) of authenticated Ceylon cinnamon (CC) were studied for antiamylase, antiglucosidase, anticholinesterases, and antiglycation and glycation reversing potential in bovine serum albumin- (BSA-) glucose and BSA-methylglyoxal models in vitro. Further, total proanthocyanidins (TP) were quantified. Results showed significant differences () between bark and leaf extracts for the studied biological activities (except antiglucosidase) and TP. BEs showed significantly high () activities for antiamylase (IC50: – μg/mL), antibutyrylcholinesterase (IC50: – μg/mL), and glycation reversing in BSA-glucose model (EC50: – μg/mL) compared to LEs. In contrast, glycation reversing in BSA-methylglyoxal (EC50: ethanol:  μg/mL) and antiglycation in both BSA-glucose (IC50: ethanol:  μg/mL) and BSA-methylglyoxal models (IC50: DCM : M:  μg/mL) were significantly high () in leaf. Compared to the reference drugs used some of the biological activities were significantly () high (BEs: BChE inhibition and ethanol leaf: BSA-glucose mediated antiglycation), some were comparable (BEs: BSA-glucose mediated antiglycation), and some were moderate (BEs and LEs: antiamylase, AChE inhibition, and BSA-MGO mediated antiglycation; DCM : M leaf: BSA-glucose mediated antiglycation). TP were significantly high () in BEs compared to LEs (BEs and LEs: – and – mg cyanidin equivalents/g extract, resp.). In conclusion, both bark and leaf of CC possess antidiabetic properties and thus may be useful in managing diabetes and its complications.

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