Visually Evoked 3-5 Hz Membrane Potential Oscillations Reduce the Responsiveness of Visual Cortex Neurons in Awake Behaving Mice

Low-frequency membrane potential (V_m) oscillations were once thought to only occur in sleeping and anesthetized states. Recently, low-frequency V_m oscillations have been described in inactive awake animals, but it is unclear whether they shape sensory processing in neurons and whether they occur during active awake behavioral states. To answer these questions, we performed two-photon guided whole-cell V_m recordings from primary visual cortex layer 2/3 excitatory and inhibitory neurons in awake mice during passive visual stimulation and performance of visual and auditory discrimination tasks. We recorded stereotyped 3–5 Hz V_m oscillations where the V_m baseline hyperpolarized as the V_m underwent high amplitude rhythmic fluctuations lasting 1–2 s in duration. When 3–5 Hz V_m oscillations coincided with visual cues, excitatory neuron responses to preferred cues were significantly reduced. Despite this disruption to sensory processing, visual cues were critical for evoking 3–5 Hz V_m oscillations when animals performed discrimination tasks and passively viewed drifting grating stimuli. Using pupillometry and animal locomotive speed as indicators of arousal, we found that 3–5 Hz oscillations were not restricted to unaroused states and that they occurred equally in aroused and unaroused states. Therefore, low-frequency V_m oscillations play a role in shaping sensory processing in visual cortical neurons, even during active wakefulness and decision making.

SIGNIFICANCE STATEMENT A neuron's membrane potential (V_m) strongly shapes how information is processed in sensory cortices of awake animals. Yet, very little is known about how low-frequency V_m oscillations influence sensory processing and whether they occur in aroused awake animals. By performing two-photon guided whole-cell recordings from layer 2/3 excitatory and inhibitory neurons in the visual cortex of awake behaving animals, we found visually evoked stereotyped 3–5 Hz V_m oscillations that disrupt excitatory responsiveness to visual stimuli. Moreover, these oscillations occurred when animals were in high and low arousal states as measured by animal speed and pupillometry. These findings show, for the first time, that low-frequency V_m oscillations can significantly modulate sensory signal processing, even in awake active animals.

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