Background. The role of Peroxiredoxin 6 (Prdx6) in brain ischemia remains unclear. Curcumin (Cur) treatment elicits neuroprotective effects against cerebral ischemic injury, and the associated mechanisms may involve Prdx6. In this study, we investigated whether Prdx6 and the transcription factor specific protein 1 (SP1) were involved in the antioxidant effect of Cur after stoke. Methods. Focal cerebral ischemic injury was induced by transient middle cerebral artery occlusion for 2 hours in male Sprague-Dawley rats treated with or without Prdx6 siRNA. Expression of Prdx6 in the penumbra was assessed by Real-Time PCR (RT-PCR), Western blot analysis, and immunoflourescent staining. In addition, infarct volume, neurological deficit score, and oxidative stress were evaluated. Prdx6 levels were also determined in the presence and absence of SP1 antagonist mithramycin A (MTM-A). Results. Cur treatment upregulated Prdx6 protein expression and the number of Prdx6-positive neuronal cells 24 hours after reperfusion. Cur treatment also attenuated oxidative stress and induced neuroprotective effects against ischemic damage, whereas the beneficial effects of Cur treatment were lost in animals treated with Prdx6-siRNA. Prdx6 upregulation by Cur treatment was abolished by SP1 antagonists MTM. Conclusions. Prdx6 upregulation by Cur treatment attenuates ischemic oxidative damage through SP1 induction in rats after stroke. This represents a novel mechanism of Cur-induced neuroprotection against cerebral ischemia.
http://ift.tt/2qQl5mw
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
▼
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
▼
Μαΐου
(5467)
-
▼
Μαΐ 17
(237)
- Cladribine to treat disease exacerbation after fin...
- Pediatric nasal surgery prior to puberty is not on...
- Klebsiella pneumoniae : a major worldwide source a...
- Perspectives for clinical use of engineered human ...
- Chemical ecology of antibiotic production by actin...
- Editorial: Bacterial pathogens, antibiotics and an...
- From Nano to Micro: using nanotechnology to combat...
- Antibiotic resistance in Staphylococcus aureus . C...
- Mining prokaryotes for antimicrobial compounds: fr...
- Molecular mechanisms of biofilm-based antibiotic r...
- Antimicrobial resistance in Mycobacterium tubercul...
- Toxin–antitoxin systems and their role in dissemin...
- Environmental and genetic modulation of the phenot...
- Formation, physiology, ecology, evolution and clin...
- The Comprehensive Complication Index (CCI®): Added...
- Intraoperative Adverse Events in Abdominal Surgery...
- Long noncoding RNAs: emerging regulators of tumor ...
- The March of Science — The True Story
- The Mapping of Predicted Triplex DNA:RNA in the Dr...
- A Generalized Linear Model for Decomposing Cis-reg...
- Essential Function of the Serine Hydroxymethyl Tra...
- FDA Warns Americans About Risk of Inaccurate Resul...
- The inimitable mouth: task-dependent kinematic dif...
- Interference between a fast-paced spatial puzzle t...
- Changing the size of a mirror-reflected hand moder...
- The power law of movement: an example of a behavio...
- Apolipoprotein E: the resilience gene
- Oculomotor prismatic training is effective in amel...
- Postural and cortical responses following visual o...
- Changing hands: persistent alterations to body ima...
- Does affective touch influence the virtual reality...
- Bimanual coordination patterns are stabilized unde...
- Rotational error in path integration: encoding and...
- Single-incision totally extraperitoneal inguinal h...
- Exome Sequencing of African-American Prostate Canc...
- Effect of resistance exercise under conditions of ...
- Impact of acetaminophen consumption and resistance...
- Flexible ammonia handling strategies using both cu...
- Pyruvate kinase inhibits proliferation during post...
- NEMO, a transcriptional target of estrogen and pro...
- Increased T cell infiltration elicited by Erk5 del...
- Expression and Histopathological Significance of D...
- Human Epicardial Fat Expresses Glucagon-Like Pepti...
- “Turning the outside in”: bariatric gastroplication
- Cortical function and corticomotoneuronal adaptati...
- Pilot Prospective Study of Post-Surgery Sleep and ...
- Association of posterior semicircular canal hypofu...
- Sex differences underlying orofacial varicella zos...
- The Development of FLT3 Inhibitors in Acute Myeloi...
- Targeting Aberrant Signaling in Myeloid Malignancies
- The Development and Use of Janus Kinase 2 Inhibito...
- Identification and Targeting of Kinase Alterations...
- A multicenter randomized trial comparing the use o...
- Bronchopulmonary Dysplasia and Perinatal Character...
- Caring for Infants and Children Following Alternat...
- Cancer Biotherapy: More Than Immunotherapy
- Circadian Gene CLOCK Affects Drug-Resistant Gene E...
- Clinical Significance and Tumor-Suppressive Functi...
- APJ Is Associated with Treatment Response in Gastr...
- First-in-Human PET/CT Imaging of Metastatic Neuroe...
- Gynecological brachytherapy for postoperative endo...
- Hangeshashinto (TJ-14) prevents radiation-induced ...
- Optimized immunohistochemistry using the D5F3 anti...
- Accept or Decline? An Analytics-Based Decision Too...
- Hepatic hemodynamics and portal flow modulation: T...
- Heart Transplantation From DCD Donors: From the Be...
- Membrane of Candida albicans as a target of berberine
- Spotlight: Angel Armor's unparalleled ballistic so...
- Identification of Novel Agents for the Treatment o...
- Histone Deacetylase Inhibitors for the Treatment o...
- Cathepsin D as a Promising Target for the Discover...
- Meet Our Editorial Board Member
- CD20-based Immunotherapy of B-cell Derived Hematol...
- NSAIDs Induced Regulation of Alternatively Spliced...
- Recent Advances in Application of Poly-Epsilon-Cap...
- Calcium-calpain Dependent Pathways Regulate Vesicu...
- Dissection and Culture of Mouse Embryonic Kidney
- Effect of Intravesical Liposome-Based Nerve Growth...
- TUmor-volume to breast-volume RAtio for improving ...
- High lymphatic vessel density and presence of lymp...
- Second-line pazopanib in patients with relapsed an...
- BAG-1 as a biomarker in early breast cancer progno...
- Transplantation of Zebrafish Pediatric Brain Tumor...
- Prospective External Validation of Three Preoperat...
- Preoperative Warming Versus no Preoperative Warmin...
- Patient Blood Management in Major Orthopedic Surge...
- Relationship Between a Sepsis Intervention Bundle ...
- Perioperative Care of the Concussed Patient: Makin...
- Use of the Fluid Challenge in Critically Ill Adult...
- An Etomidate Analogue With Less Adrenocortical Sup...
- Cancellation of Elective Cases in a Recently Opene...
- Cerebral Oxygen Saturation in Children With Congen...
- A 5-mC Dot Blot Assay Quantifying the DNA Methylat...
- A Simple Chamber for Long-term Confocal Imaging of...
- Spotlight: Angel Armor's unparalleled ballistic so...
- The effect of repeated freeze-thaw cycles on human...
- Brain ultrasound rehearsal before surgery: A pilot...
- Detailed anatomy of the abducens nerve in the late...
- Histology of the Distal Dural Ring
- Injury to the infrapatellar branch of the saphenou...
-
▼
Μαΐ 17
(237)
-
►
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
- ► Φεβρουαρίου (793)
Αναζήτηση αυτού του ιστολογίου
Τετάρτη 17 Μαΐου 2017
Prdx6 Upregulation by Curcumin Attenuates Ischemic Oxidative Damage via SP1 in Rats after Stroke
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
https://ift.tt/2MQ8Ai8
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.