Identification of embryo-fetal cells in celomic fluid using morphological and short-tandem repeats analysis

Abstract

Objective

The main problem to wide acceptability of celocentesis as earlier prenatal diagnosis is contamination of the sample by maternal cells. The objective of this study was to investigate the cellular composition of celomic fluid for morphological discrimination between maternal and embryo-fetal cells.

Method

Celomic fluids were aspired by ultrasound-guided transcervical celocentesis at 7-9 weeks' gestation from singleton pregnancies before surgical termination for psychological reasons. DNA was extracted from celomic fluid cells showed the same morphology and quantitative fluorescentPCR assay was performed to evaluate their fetal or maternal origin.

Results

Six different types of non-hematological maternal and four different types of embryo-fetal cells were detected. The most common maternal cells were of epithelial origin. The majority of embryo-fetal cells were roundish with a nucleus located in an eccentric position near the wall. These cells were considered to be erythroblasts, probably derived from the yolk sac that serves as the initial site of erythropoiesis.

Conclusions

The combined use of morphology and DNA analysis make it possible to select and isolate embryo-fetal cells, even when maternal contamination is high. This development provides the opportunity for the use of celocentesis for early prenatal diagnosis of genetic diseases and application of array-CGH. This article is protected by copyright. All rights reserved.

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