Characterization of protein-based risk signature to predict prognosis and evaluate the tumor immune environment in breast cancer

AlexandrosSfakianakis shared this article with you from Inoreader Characterization of protein-based risk signature to predict prognosis and evaluate the tumor immune environment in breast cancer via Breast Cancer CONCLUSION: This study established an effective prognostic proteomics signature with reliable predictive performance for survival, immune activity, and drug sensitivity. It might provide a novel perspective into the protein function in BC, and guide the individual treatment strategies for BC patients.PMID:36732487 | DOI:10.1007/s12282-023-01435-8 (Source: Breast Cancer) View on Web

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Acute Posterior Multifocal Placoid Pigment Epitheliopathy: A Rare Presentation of Anklylosing Spondylitis or a Paradoxical Reaction to Secukinumab?

AlexandrosSfakianakis shared this article with you from Inoreader Acute Posterior Multifocal Placoid Pigment Epitheliopathy: A Rare Presentation of Anklylosing Spondylitis or a Paradoxical Reaction to Secukinumab? via Ocular Immunology and Inflammation CONCLUSION: In AS patients, posterior uveitis can manifest as APMPPE. It should be recorded as an entity to be considered in the differential diagnosis.PMID:36608284 | DOI:10.1080/09273948.2022.2150225 (Source: Ocular Immunology and Inflammation) View on Web

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Onychomycosis associated with diabetic foot syndrome: a systematic review

AlexandrosSfakianakis shared this article with you from Inoreader Onychomycosis associated with diabetic foot syndrome: a systematic review via Mycoses Abstract Background A systematic review was conducted to investigate the prevalence of onychomycosis in patients with diabetes. The association of onychomycosis with risk factors in patients with diabetic foot syndrome was also examined. Methods The recommendations in the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) checklist were applied, and the included studies were assessed using the Strengthening the Reporting of Observational studies in Epidemiology (STROBE) method. Searches were conducted in October 2022 using PubMed (Medline) and Scopus for clinical studies, clinical trials, comparative studies, observational studies, and randomised clinical trials or controlled clinical trials addressing the prevalence and consequences of onychomycosis in patients with diabetes, diagnoses, or treatments. Two authors performed the study selection and data extraction, and any discrepancies between the two reviewers were resolved through discussion with a third reviewer. Results The systematic review included nine studies that met the inclusion criteria, and these studies enrolled 5426 patients with diabetes. Among these patients, 28.55% had onychomycosis that was mainly caused by Trichophyton rubrum. A significant association was found between the occurrence of onychomycosis and the presence of diabetic neuropathy (p=0.012) and elevated glycosylated haemoglobin values (p=0.039). There was no significant association between onychomycosis and ulceration (p=0.185). Eight studies had a grade 4 level of evidence and a grade C recommendation, and one study had a grade 1b level of evidence and a grade A recommendation. Conclusion The information described in the literature is insufficient and heterogeneous regarding the association of risk factors and ulceration in patients with diabetic foot compared with developing onychomycosis. There is also a need to implement onychomycosis diagnostic testing instead of relying only on a clinical diagnosis. Additional prospective, randomised, comparative studies are needed to increase the quality of studies in the literature. View on Web

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PPAR-γ activation promotes xenogenic bioroot regeneration by attenuating the xenograft induced-oxidative stress

AlexandrosSfakianakis shared this article with you from Inoreader PPAR-γ activation promotes xenogenic bioroot regeneration by attenuating the xenograft induced-oxidative stress via International Journal of Oral Science - Issue International Journal of Oral Science, Published online: 16 February 2023; doi:10.1038/s41368-023-00217-4 PPAR-γ activation promotes xenogenic bioroot regeneration by attenuating the xenograft induced-oxidative stress View on Web

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In vivo PIWI slicing in mouse testes deviates from rules established in vitro [LETTER TO THE EDITOR]

AlexandrosSfakianakis shared this article with you from Inoreader In vivo PIWI slicing in mouse testes deviates from rules established in vitro [LETTER TO THE EDITOR] via RNA current issue Argonautes are small RNA-binding proteins, with some having small RNA-guided endonuclease (slicer) activity that cleaves target nucleic acids. One cardinal rule that is structurally defined is the inability of slicers to cleave target RNAs when nucleotide mismatches exist between the paired small RNA and the target at the cleavage site. Animal-specific PIWI clade Argonautes associate with PIWI-interacting RNAs (piRNAs) to silence transposable elements in the gonads, and this is essential for fertility. We previously demonstrated that purified endogenous mouse MIWI fails to cleave mismatched targets in vitro. Surprisingly, here we find using knock-in mouse models that target sites with cleavage-site mismatches at the 10th and 11th piRNA nucleotides are precisely sliced in vivo. This is identical to the slicing outcome in knock-in mice where targets are base-paired perfectly with the piRNA. Additionally, we find that pachytene piRNA-guided slicing in both these situations failed to initiate phased piRNA production from the specific target mRNA we studied. Instead, the two slicer cleavage fragments were retained in PIWI proteins as pre-piRNA and 17–19 nt by-product fragments. Our results indicate that PIWI slicing rules established in vitro are not respected in vivo, and that all targets of PIWI slicing are not substrates for piRNA biogenesis. View on Web

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Designing a Prolonged Method of Therapeutic Delivery to Support Rehabilitation From Ototoxic Damage in a Schwann Cell Model

AlexandrosSfakianakis shared this article with you from Inoreader Designing a Prolonged Method of Therapeutic Delivery to Support Rehabilitation From Ototoxic Damage in a Schwann Cell Model via Otology & Neurotology - Published Ahead-of-Print Hypothesis The ototoxicity of gentamicin and cisplatin can be evaluated with a Schwann cell model to screen for otoprotective agents that can be encapsulated into poly (lactic-co-glycolic acid) (PLGA) microparticles for drug delivery to the inner ear. Background Aminoglycosides and cisplatin are widely prescribed but known to cause ototoxicity. There is strong evidence that compromise to Schwann cells ensheathing inner ear afferent neurons results in inner ear dysfunction mimicking drug-induced ototoxicity. There is a need for a model for ototoxic demyelination to screen medications for protective potential and to subsequently target and tune the delivery of any promising agents. Methods RT4-D6P2T rat schwannoma cells were used as a Schwann cell model to assess gentamicin and cisplatin toxicity and to screen for protective agents. Cell viability was evaluated with the MTT cell proliferation assay. N-acetylcysteine (NAC) was encapsulated into a PLGA microparticle, and its elution profile was determined. Results The estimated 50% lethal concentration dose for gentamicin was 805.6 μM, which was 46-fold higher than that for cisplatin (17.5 μM). In several trials, cells dosed with NAC and cisplatin demonstrated a 22.6% (p View on Web

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Motor Imagery EEG Decoding Based on Multi-Scale Hybrid Networks and Feature Enhancement

AlexandrosSfakianakis shared this article with you from Inoreader Motor Imagery EEG Decoding Based on Multi-Scale Hybrid Networks and Feature Enhancement via Neural Systems and Rehabilitation Engineering, IEEE Transactions on - new TOC Motor Imagery (MI) based on Electroencephalography (EEG), a typical Brain-Computer Interface (BCI) paradigm, can communicate with external devices according to the brain's intentions. Convolutional Neural Networks (CNN) are gradually used for EEG classification tasks and have achieved satisfactory performance. However, most CNN-based methods employ a single convolution mode and a convolution kernel size, which cannot extract multi-scale advanced temporal and spatial features efficiently. What's more, they hinder the further improvement of the classification accuracy of MI-EEG signals. This paper proposes a novel Multi-Scale Hybrid Convolutional Neural Network (MSHCNN) for MI-EEG signal decoding to improve classification performance. The two-dimensional convolution is used to extract temporal and spatial features of EEG signals and the one-dimensional convolution is used to extract advanced temporal features of EEG signals. In addition, a channel coding method is proposed to improve the expression capacity of the spatiotemporal characteristics of EEG signals. We evaluate the performance of the proposed method on the dataset collected in the laboratory and BCI competition IV 2b, 2a, and the average accuracy is at 96.87%, 85.25%, and 84.86%, respectively. Compared with other advanced methods, our proposed method achieves higher classification accuracy. Then we use the proposed method for an online experiment and design an intelligent artificial limb control system. The proposed method effectively extracts EEG signals' advanced temporal and spatial features. Additionally, we design an online recognition system, which contributes to the further development of the BCI system. View on Web

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