Novel Object Exploration as a Potential Assay for Higher Order Repetitive Behaviors in Mice

Higher order restricted, repetitive behaviors (RRBs) disrupt the lives of affected individuals. These behaviors are challenging to model in rodents, making basic biomedical research into potential treatments or interventions for RRBs difficult. Here we describe novel object exploration as a potential assay for higher order RRBs in mice.

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Protective effect of curcumin on TNBS-induced intestinal inflammation is mediated through the JAK/STAT pathway

Curcumin displays a protective role in rat models of intestinal inflammation. However, the mechanism of how curcumin affects on intestinal inflammation is less known. The purpose of the current study is to exp...

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Anti-inflammatory effects of Zea mays L. husk extracts

Zea mays L. (Z. mays) has been used for human consumption in the various forms of meal, cooking oil, thickener in sauces and puddings, sweetener in processed food and beverage products...

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A systematic review and meta-analysis of acupuncture for improving learning and memory ability in animals

Memory loss is the most prominent symptoms of brain aging, but there is currently no evidence-based treatment strategy. Acupuncture has been widely used in China and the effectiveness for improving learning an...

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Effects of LeY glycan expression on embryo implantation

OBJECTIVE: To investigate the correlation between LeY glycan expression and embryo implantation.

MATERIALS AND METHODS: Uterine epithelial cells before implantation were transfected with FUT1siRNA to inhibit FUT1 (the gene encoding the key enzyme of LeY synthesis) expression and treated with 10 ng/ml leukemia inhibitory factor (LIF). Murine embryo implantation model in vitro was prepared by late blastocysts with identical morphology and treated uterine epithelial cells co-culture. Using RT-PCR, dot blot and observation of embryo attachment to analyze FUT1 gene expression and LeY synthesis of uterine epithelial cells and studied further the correlation of LeY expression level and embryo implantation.

RESULTS: FUT1 gene expression and LeY synthesis declined after cells were transfected with FUT1siRNA, and LIF promoted FUT1 expression and LeY synthesis. After expression of FUT1 gene was inhibited, attachment rate of embryos lowered, but LIF up-regulated FUT1 expression and increased the attachment rate of embryos.

CONCLUSIONS: These results indicated regulating FUT1 expression affected LeY synthesis, and then LeY regulated the recognition and attachment of uterus-embryo and participates in embryo implantation further.

L'articolo Effects of LeY glycan expression on embryo implantation sembra essere il primo su European Review.

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miRNA-15a-5p regulates VEGFA in endometrial mesenchymal stem cells and contributes to the pathogenesis of endometriosis

OBJECTIVE: miRNAs have been recently reported to contribute to the etiology of endometriosis in stem cells. However, the mechanisms remain unclear.

The aim of this investigation is to explore the expression of miR-15a-5p and VEGFA in endometrial samples from patients with or without endometriosis. And then examine the regulation by miR-15a-5p on the expression of VEGFA.

PATIENTS AND METHODS: Here we collected 31 endometrial samples from patients with or without endometriosis and characterized the miRNAs expression profiles of these two groups. Then, we investigated the regulation by microRNA-15a-5p (miR-15a-5p) on the expression of vascular endothelial growth factor (VEGF) in endometrial mesenchymal stem cells.

RESULTS: It was demonstrated that there was dramatically down-regulation of miR-15a-5p in the patients with endometriosis, compared with control patients. Moreover, we found that the up-regulation of miR-15a-5p suppressed cell proliferation, migration and invasion of these ectopic stem cells by targeting the 3′ untranslated region of VEGFA.

CONCLUSIONS: Taken together, this newly identified miR-15a-5p module provides a new avenue to the understanding of the processes of endometriosis development, especially proliferation, motility as well as angiogenesis, and may facilitate the development of potential therapeutics against endometriosis.

L'articolo miRNA-15a-5p regulates VEGFA in endometrial mesenchymal stem cells and contributes to the pathogenesis of endometriosis sembra essere il primo su European Review.

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Measuring phacoemulsification groove depth using probe tip dimensions and biometry lens thickness readings

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