Medicine by Alexandros G.Sfakianakis: Μαΐου 2016

Τρίτη 31 Μαΐου 2016

Phytochemicals and Medicinal Properties of Indigenous Tropical Fruits with Potential for Commercial Development

Hundreds of fruit-bearing trees are native to Southeast Asia, but many of them are considered as indigenous or underutilized. These species can be categorized as indigenous tropical fruits with potential for commercial development and those possible for commercial development. Many of these fruits are considered as underutilized unless the commercialization is being realized despite the fact that they have the developmental potential. This review discusses seven indigenous tropical fruits from 15 species that have been identified, in which their fruits are having potential for commercial development. As they are not as popular as the commercially available fruits, limited information is found. This paper is the first initiative to provide information on the phytochemicals and potential medicinal uses of these fruits. Phytochemicals detected in these fruits are mainly the phenolic compounds, carotenoids, and other terpenoids. Most of these phytochemicals are potent antioxidants and have corresponded to the free radical scavenging activities and other biological activities of the fruits. The scientific research that covered a broad range of in vitro to in vivo studies on the medicinal potentials of these fruits is also discussed in detail. The current review is an update for researchers to have a better understanding of the species, which simultaneously can provide awareness to enhance their commercial value and promote their utilization for better biodiversity conservation.

from #Medicine via ola Kala on Inoreader http://ift.tt/1Y17LEA
via IFTTT

Designing piperlongumine-directed anticancer agents by an electrophilicity-based prooxidant strategy: A mechanistic investigation

Publication date: August 2016
Source:Free Radical Biology and Medicine, Volume 97
Author(s): Wen-Jing Yan, Qi Wang, Cui-Hong Yuan, Fu Wang, Yuan Ji, Fang Dai, Xiao-Ling Jin, Bo Zhou
Piperlongumine (PL), a natural electrophilic alkaloid bearing two α, β-unsaturated imides, is a promising anticancer molecule by targeting the stress response to reactive oxygen species (ROS). Considering that ROS generation depends on electrophilicity of PL, PL-CL was designed as its analog by introducing the α-substituent chlorine on the lactam ring to increase moderately its electrophilicity. In comparison with the parent molecule, this molecule was identified as a stronger ROS (O2^∙− and H2O2) inducer and cytotoxic agent, and manifested more than 15-fold selectivity toward A549 cells over normal WI-38 cells. Mechanistic study uncovers for the first time that the selenoprotein thioredoxin reductase (TrxR) is one of the targets by which PL-CL promotes the ROS generation. Stronger intracellular TrxR inhibition and higher accumulation of ROS (O2^∙− and H2O2) are responsible for more effective S-phase arrest and mitochondria-mediated apoptotic induction of A549 cells by PL-CL than PLvia p53-p21-cyclinA/CDK2 and ASK1-JNK/p38 signaling cascade pathways, respectively. This work provides an example of successfully designing PL-directed anticancer agent by an electrophilicity-based prooxidant (ROS-generating agent) strategy and gives added confidence for extending this strategy to other natural products.

Graphical abstract

from #Medicine via ola Kala on Inoreader http://ift.tt/1Ub3Gba
via IFTTT

Detailed protocol to assess in vivo and ex vivo myeloperoxidase activity in mouse models of vascular inflammation and disease using hydroethidine

Publication date: August 2016
Source:Free Radical Biology and Medicine, Volume 97
Author(s): Jihan Talib, Ghassan J. Maghzal, David Cheng, Roland Stocker
Myeloperoxidase (MPO) activity contributes to arterial inflammation, vascular dysfunction and disease, including atherosclerosis. Current assessment of MPO activity in biological systems in vivo utilizes 3-chlorotyrosine (3-Cl-Tyr) as a biomarker of hypochlorous acid (HOCl) and other chlorinating species. However, 3-Cl-Tyr is formed in low yield and is subject to further metabolism. Recently, we reported a method to selectively assess MPO-activity in vivo by measuring the conversion of hydroethidine to 2-chloroethidium (2-Cl-E⁺) by liquid chromatography with tandem mass spectrometry (LC–MS/MS) (J. Biol. Chem., 289, 2014, pp. 5580–5595). The hydroethidine-based method has greater sensitivity for MPO activity than measurement of 3-Cl-Tyr. The current methods paper provides a detailed protocol to determine in vivo and ex vivo MPO activity in arteries from mouse models of vascular inflammation and disease by utilizing the conversion of hydroethidine to 2-Cl-E⁺. Procedures for the synthesis of standards, preparation of tissue homogenates and the generation of 2-Cl-E⁺ are also provided in detail, as are the conditions for LC–MS/MS detection of 2-Cl-E⁺.

from #Medicine via ola Kala on Inoreader http://ift.tt/1Ub3B7r
via IFTTT

Natural resource-based industries and prostate cancer risk in Northeastern Ontario: a case-control study

Objective

Prostate cancer continues to be the most commonly diagnosed cancer in men, and there is limited knowledge on its preventable risk factors. A number of occupational exposures in natural resource-based industries are suspected to be related to prostate cancer risk. This study investigates associations between employment in these industries and prostate cancer.

Methods

Data were from a population-based, case–control study previously conducted in Northeastern Ontario. Incident cases (N=760) aged 45–85 years and diagnosed with prostate cancer between 1995 and 1998 were identified from the Ontario Cancer Registry. Controls (N=1632) were recruited using telephone listings, and were frequency matched to cases by age. Lifetime occupational history was collected for all participants. Logistic regression was used to estimate ORs and their associated 95% CIs.

Results

Elevated risks were observed for employment in forestry and logging industries (OR=1.87, 95% CI 1.32 to 2.73) and occupations (OR=1.71, 95% CI 1.24 to 2.35), and these risks increased with duration of employment for ≥10 years. Elevated risks were also found for employment in wood products industries (OR=1.45, 95% CI 1.07 to 1.97), and paper and allied products industries (OR=1.43, 95% CI 1.03 to 2.00), and when duration of employment was ≥10 years. There were also elevated risks in agriculture and mining-related work; however, these findings were not consistent across industry and occupation categories.

Conclusions

Prostate cancer risk may be associated with work in several natural resource industries, primarily in the forest industries. To further evaluate observed associations, studies should focus on natural resource-based exposures in larger populations with improved exposure assessment.

from #Medicine via ola Kala on Inoreader http://ift.tt/1O2eNH0
via IFTTT

Simple benchmark for mesothelioma projection for Great Britain

Background

It is of considerable interest to forecast the future burden of mesothelioma mortality. Data on deaths are available, whereas no measure of asbestos exposure is available.

Methods

We compare two Poisson models: a response-only model with an age-cohort specification and a multinomial model with epidemiologically motivated frequencies.

Results

The response-only model has 5% higher peak mortality than the dose–response model. The former performs slightly better in out-of-sample comparison.

Conclusions

Mortality is predicted to peak at about 2100 deaths around 2017 among males in cohorts until 1966 and below 90 years of age. The response-only model is a simple benchmark that forecasts just as well as more complicated models.

from #Medicine via ola Kala on Inoreader http://ift.tt/1Ub3CrS
via IFTTT

Subchronic oral administration of crude khat extract (Catha edulis forsk) induces schizophernic-like symptoms in mice

Chewing fresh leaves of the khat plant (Catha edulis forsk) is a deep rooted and widespread habit in East Africa and the Middle East. Although a body of knowledge exists about the adverse effects of khat on healt...

from #Medicine via ola Kala on Inoreader http://ift.tt/1UaYzYw
via IFTTT

Buyang Huanwu Decoction attenuates H2O2-induced apoptosis by inhibiting reactive oxygen species-mediated mitochondrial dysfunction pathway in human umbilical vein endothelial cells

Apoptosis of endothelial cells caused by reactive oxygen species plays an important role in ischemia/reperfusion injury after cerebral infarction. Buyang Huanwu Decoction (BYHWD) has been used to treat stroke ...

from #Medicine via ola Kala on Inoreader http://ift.tt/1VsU4xw
via IFTTT

Amomum cardamomum L. ethyl acetate fraction protects against carbon tetrachloride-induced liver injury via an antioxidant mechanism in rats

Medicinal herb-derived drug development has become important in the relief of liver pathology. Amomun cardamomum is traditionally used therapeutically in Korea to treat various human ailments including dyspepsia,...

from #Medicine via ola Kala on Inoreader http://ift.tt/1UaYB2u
via IFTTT

Cristacarpin promotes ER stress-mediated ROS generation leading to premature senescence by activation of p21 waf-1

Abstract

Stress-induced premature senescence (SIPS) is quite similar to replicative senescence that is committed by cells exposed to various stress conditions viz. ultraviolet radiation (DNA damage), hydrogen peroxide (oxidative stress), chemotherapeutic agents (cytotoxic threat), etc. Here, we report that cristacarpin, a natural product obtained from the stem bark of Erythrina suberosa, promotes endoplasmic reticulum (ER) stress, leading to sub-lethal reactive oxygen species (ROS) generation and which eventually terminates by triggering senescence in pancreatic and breast cancer cells through blocking the cell cycle in the G1 phase. The majority of cristacarpin-treated cells responded to conventional SA-β-gal stains; showed characteristic p21^waf1 upregulation along with enlarged and flattened morphology; and increased volume, granularity, and formation of heterochromatin foci—all of these features are the hallmarks of senescence. Inhibition of ROS generation by N-acetyl-l-cysteine (NAC) significantly reduced the expression of p21^waf1, confirming that the modulation in p21^waf1 by anti-proliferative cristacarpin was ROS dependent. Further, the elevation in p21^waf1 expression in PANC-1 and MCF-7 cells was consistent with the decrease in the expression of Cdk-2 and cyclinD1. Here, we provide evidence that cristacarpin promotes senescence in a p53-independent manner. Moreover, cristacarpin treatment induced p38MAPK, indicating the ROS-dependent activation of the MAP kinase pathway, and thus abrogates the tumor growth in mouse allograft tumor model.

from #Medicine via ola Kala on Inoreader http://ift.tt/1TV31yq
via IFTTT

Antiatherogenic Effect of Camellia japonica Fruit Extract in High Fat Diet-Fed Rats

Hypercholesterolemia is a well-known etiological factor for cardiovascular disease and a common symptom of most types of metabolic disorders. Camellia japonica is a traditional garden plant, and its flower and seed have been used as a base oil of traditional cosmetics in East Asia. The present study was carried out to evaluate the effect of C. japonica fruit extracts (CJF) in a high fat diet- (HFD-) induced hypercholesterolemic rat model. CJF was administered orally at three different doses: 100, 400, and 800 mg·kg−1·day−1 (CJF 100, 400, and 800, resp.). Our results showed that CJF possessed strong cholesterol-lowering potency as indicated by the decrease in serum total cholesterol (TC), triglyceride (TG), and low-density lipoprotein (LDL), accompanied by an increase in serum high-density lipoprotein (HDL). Furthermore, CJF reduced serum lipid peroxidation by suppressing the formation of thiobarbituric acid reactive substance. In addition, oil red O (ORO) staining of rat arteries showed decreased lipid-positive staining in the CJF-treated groups compared to the control HFD group. Taken together, these results suggest that CJF could be a potent herbal therapeutic option and source of a functional food for the prevention and treatment of atherosclerosis and other diseases associated with hypercholesterolemia.

from #Medicine via ola Kala on Inoreader http://ift.tt/1r0m6nl
via IFTTT

Inductive Effect of Palmatine on Apoptosis in RAW 264.7 Cells

Osteoporosis is a serious public health problem characterized by low bone density and deterioration of the bone microarchitecture. Current treatment options target either osteoclast resorption or osteoblast formation. It has been reported that berberine, a close structural analog of palmatine, inhibited bone loss in an osteoporosis model. In this study, osseous metabolism was observed in vitro with osteoclast bone resorbing cells. We proved that mouse preosteoclastic cell line (RAW 264.7) has a higher sensitivity to palmatine than mouse osteoblastic cell line (MC3T3-E1); the cell survival rates significantly decreased at 40 μM palmatine. The level, a metabolic product of nitric monoxide (NO), and iNOS mRNA expression, an osteoclast with NO induced enzyme, also increased with higher dosage of palmatine. Furthermore, it was recognized that the cell viability decrease from palmatine was caused by apoptosis rather than necrosis. Additionally, osteoclast apoptosis from palmatine did not occur when iNOS was inhibited with -nitro-L-arginine methyl ester hydrochloride (pan NOS inhibitor). These results indicate that palmatine plays an important role in osteoclast apoptosis via the NOS system. Hence, palmatine could be considered as a viable pharmaceutical candidate for osteoporosis bone resorption inhibitor.

from #Medicine via ola Kala on Inoreader http://ift.tt/1Urr2fd
via IFTTT

IJMS, Vol. 17, Pages 700: Pesticides Drive Stochastic Changes in the Chemoreception and Neurotransmission System of Marine Ectoparasites

Scientific efforts to elucidate the mechanisms of chemical communication between organisms in marine environments are increasing. This study applied novel molecular technology to outline the effects of two xenobiotic drugs, deltamethrin (DM) and azamethiphos (AZA), on the neurotransmission system of the copepod ectoparasite Caligus rogercresseyi. Transcriptome sequencing and bioinformatics analyses were conducted to evaluate treatment effects on the glutamatergic synaptic pathway of the parasite, which is closely related to chemoreception and neurotransmission. After drug treatment with DM or AZA, stochastic mRNA expression patterns of glutamatergic synapse pathway components were observed. Both DM and AZA promoted a down-regulation of the glutamate-ammonia ligase, and DM activated a metabotropic glutamate receptor that is a suggested inhibitor of neurotransmission. Furthermore, the delousing drugs drove complex rearrangements in the distribution of mapped reads for specific metabotropic glutamate receptor domains. This study introduces a novel methodological approach that produces high-quality results from transcriptomic data. Using this approach, DM and AZA were found to alter the expression of numerous mRNAs tightly linked to the glutamatergic signaling pathway. These data suggest possible new targets for xenobiotic drugs that play key roles in the delousing effects of antiparasitics in sea lice.

from #Medicine via ola Kala on Inoreader http://ift.tt/25ypLvH
via IFTTT

Impact of the increased adoption of prenatal cfDNA screening on non-profit patient advocacy organizations in the United States

Abstract

The "Stakeholder Perspectives on Noninvasive Prenatal Genetic Screening" Symposium was held in conjunction with the 2015 annual meeting of the International Society for Prenatal Diagnosis. During the day-long meeting, a panel of patient advocacy group (PAG) representatives discussed concerns and challenges raised by prenatal cfDNA screening, which has resulted in larger demands upon PAGs from concerned patients receiving prenatal cfDNA screening results. Prominent concerns included confusion about the accuracy of cfDNA screening and a lack of patient education resources about genetic conditions included in cfDNA screens. Some of the challenges faced by PAGs included funding limitations; lack of consistently implemented standards of care and oversight; diverse perspectives among PAGs and questions about neutrality; and lack of access to training and genetic counselors. PAG representatives also put forward suggestions for addressing these challenges, including improving educational and PAG funding and increasing collaboration between PAGs and the medical community.

from #Medicine via ola Kala on Inoreader http://ift.tt/22vIHWF
via IFTTT

Vision 20/20: Molecular-guided surgical oncology based upon tumor metabolism or immunologic phenotype: Technological pathways for point of care imaging and intervention

Surgical guidance with fluorescence has been demonstrated in individual clinical trials for decades, but the scientific and commercial conditions exist today for a dramatic increase in clinical value. In the past decade, increased use of indocyanine green based visualization of vascular flow, biliary function, and tissue perfusion has spawned a robust growth in commercial systems that have near-infrared emission imaging and video display capabilities. This recent history combined with major preclinical innovations in fluorescent-labeled molecular probes, has the potential for a shift in surgical practice toward resection guidance based upon molecular information in addition to conventional visual and palpable cues. Most surgical subspecialties already have treatment management decisions partially based upon the immunohistochemical phenotype of the cancer, as assessed from molecular pathology of the biopsy tissue. This phenotyping can inform the surgical resection process by spatial mapping of these features. Further integration of the diagnostic and therapeutic value of tumor metabolism sensing molecules or immune binding agents directly into the surgical process can help this field mature. Maximal value to the patient would come from identifying the spatial patterns of molecular expression in vivo that are well known to exist. However, as each molecular agent is advanced into trials, the performance of the imaging system can have a critical impact on the success. For example, use of pre-existing commercial imaging systems are not well suited to image receptor targeted fluorophores because of the lower concentrations expected, requiring orders of magnitude more sensitivity. Additionally the imaging system needs the appropriate dynamic range and image processing features to view molecular probes or therapeutics that may have nonspecific uptake or pharmacokinetic issues which lead to limitations in contrast. Imaging systems need to be chosen based upon objective performance criteria, and issues around calibration, validation, and interpretation need to be established before a clinical trial starts. Finally, as early phase trials become more established, the costs associated with failures can be crippling to the field, and so judicious use of phase 0 trials with microdose levels of agents is one viable paradigm to help the field advance, but this places high sensitivity requirements on the imaging systems used. Molecular-guided surgery has truly transformative potential, and several key challenges are outlined here with the goal of seeing efficient advancement with ideal choices. The focus of this vision 20/20 paper is on the technological aspects that are needed to be paired with these agents.

from #Medicine via ola Kala on Inoreader http://ift.tt/1XbGNuQ
via IFTTT

Automated multistructure atlas-assisted detection of lymph nodes using pelvic MR lymphography in prostate cancer patients

Purpose:

To investigate whether atlas-based anatomical information can improve a fully automated lymph node detection system for pelvic MR lymphography (MRL) images of patients with prostate cancer.

Methods:

Their data set contained MRL images of 240 prostate cancer patients who had an MRL as part of their clinical work-up between January 2008 and April 2010, with ferumoxtran-10 as contrast agent. Each MRL consisted of at least a 3D T1-weighted sequence, a 3D T2*-weighted sequence, and a FLASH-3D sequence. The reference standard was created by two expert readers, reading in consensus, who annotated and interactively segmented the lymph nodes in all MRL studies. A total of 5089 lymph nodes were annotated. A fully automated computer-aided detection (CAD) system was developed to find lymph nodes in the MRL studies. The system incorporates voxel features based on image intensities, the Hessian matrix, and spatial position. After feature calculation, a GentleBoost-classifier in combination with local maxima detection was used to identify lymph node candidates. Multiatlas based anatomical information was added to the CAD system to assess whether this could improve performance. Using histogram analysis and free-receiver operating characteristic analysis, this was compared to a strategy where relative position features were used to encode anatomical information.

Results:

Adding atlas-based anatomical information to the CAD system reduced false positive detections both visually and quantitatively. Median likelihood values of false positives decreased significantly in all annotated anatomical structures. The sensitivity increased from 53% to 70% at 10 false positives per lymph node.

Conclusions:

Adding anatomical information through atlas registration significantly improves an automated lymph node detection system for MRL images.

from #Medicine via ola Kala on Inoreader http://ift.tt/1RIXCF4
via IFTTT

Oxidative Stress Relevance in the Pathogenesis of the Rheumatoid Arthritis: A Systematic Review

Rheumatoid arthritis (RA) is an autoimmune inflammatory disease whose pathogenic mechanisms remain to be elucidated. The oxidative stress and antioxidants play an important role in the disease process of RA. The study of oxidants and antioxidants biomarkers in RA patients could improve our understanding of disease pathogenesis; likely determining the oxidative stress levels in these patients could prove helpful in assessing disease activity and might also have prognostic implications. To date, the usefulness of oxidative stress biomarkers in RA patients is unclear and the evidence supporting them is heterogeneous. In order to resume and update the information in the status of oxidants and antioxidants and their connection as biomarkers in RA, we performed a systematic literature search in the PubMed database, including clinical trials published in the last five years using the word combination "rheumatoid arthritis oxidative stress". In conclusion, this review supports the fact that the oxidative stress is an active process in RA pathogenesis interrelated to other better known pathogenic elements. However, some controversial results preclude a definite conclusion.

from #Medicine via ola Kala on Inoreader http://ift.tt/1WWrLbM
via IFTTT

IJMS, Vol. 17, Pages 700: Pesticides Drive Stochastic Changes in the Chemoreception and Neurotransmission System of Marine Ectoparasites

Δευτέρα 30 Μαΐου 2016

A Meta-Analysis of Randomized Controlled Trials of Yiqi Yangyin Huoxue Method in Treating Diabetic Nephropathy

Objective. The purpose of this systematic review is to evaluate the evidence of Yiqi Yangyin Huoxue Method for diabetic nephropathy. Methods. 11 electronic databases, through September 2015, were searched to identify randomized controlled trials of Yiqi Yangyin Huoxue Method for diabetic nephropathy. The quality of the included trials was assessed using the Jadad scale. Results. 26 randomized controlled trials were included in our review. Of all the included trials, most of them were considered as high quality. The aggregated results suggested that Yiqi Yangyin Huoxue Method is beneficial to diabetic nephropathy in bringing down the microalbuminuria (SMD = −0.98, 95% CI −1.22 to −0.74), serum creatinine (SMD = −0.56, 95% CI −0.93 to −0.20), beta-2 microglobulin (MD = 0.06, 95% CI 0.01 to 0.12), fasting plasma glucose (MD = −0.35, 95% CI −0.62 to −0.08), and 2-hour postprandial blood glucose (MD = 1.13, 95% CI 0.07 to 2.20), but not in decreasing blood urea nitrogen (SMD = −0.72, 95% CI −1.47 to 0.02) or 2-hour postprandial blood glucose (SMD = −0.48, 95% CI −1.01 to 0.04). Conclusions. Yiqi Yangyin Huoxue Method should be a valid complementary and alternative therapy in the management of diabetic nephropathy, especially in improving UAER, serum creatinine, fasting blood glucose, and beta-2 microglobulin. However, more studies with long follow-up are warrant to confirm the current findings.

from #Medicine via ola Kala on Inoreader http://ift.tt/1X8b51Q
via IFTTT

The formation, function and regulation of amyloids: insights from structural biology

Abstract

Amyloid diseases are characterized by the accumulation of insoluble, β-strand-rich aggregates. The underlying structural conversions are closely associated with cellular toxicity, but can also drive the formation of functional protein assemblies. In recent years, studies in the field of structural studies have revealed astonishing insights into the origins, mechanisms and implications of amyloid formation. Notably, high-resolution crystal structures of peptides in amyloid-like fibrils and prefibrillar oligomers have become available despite their challenging chemical nature. Nuclear magnetic resonance spectroscopy has revealed that dynamic local polymorphisms in the benign form of the prion protein affect the transformation into amyloid fibrils and the transmissibility of prion diseases. Studies of the structures and interactions of chaperone proteins help us to understand how the cellular proteostasis network is able to recognize different stages of aberrant protein folding and prevent aggregation. In this review, we will focus on recent developments that connect the different aspects of amyloid biology and discuss how understanding the process of amyloid formation and the associated defence mechanisms can reveal targets for pharmacological intervention that may become the first steps towards clinically viable treatment strategies.

from #Medicine via ola Kala on Inoreader http://ift.tt/1PbOhez
via IFTTT

Autotaxin interacts with lipoprotein(a) and oxidized phospholipids in predicting the risk of calcific aortic valve stenosis in patients with coronary artery disease

Abstract

Background

Studies have shown that lipoprotein(a) [Lp(a)], an important carrier of oxidized phospholipids, is causally related to calcific aortic valve stenosis (CAVS). Recently, we found that Lp(a) mediates the development of CAVS through autotaxin (ATX).

Objective

To determine the predictive value of circulating ATX mass and activity for CAVS.

Methods

We performed a case-control study in 300 patients with coronary artery disease (CAD). Patients with CAVS plus CAD (cases, n = 150) were age- and gender-matched (1 : 1) to patients with CAD without aortic valve disease (controls, n = 150). ATX mass and enzymatic activity and levels of Lp(a) and oxidized phospholipids on apolipoprotein B-100 (OxPL-apoB) were determined in fasting plasma samples.

Results

Compared to patients with CAD alone, ATX mass (P < 0.0001), ATX activity (P = 0.05), Lp(a) (P = 0.003) and OxPL-apoB (P < 0.0001) levels were elevated in those with CAVS. After adjustment, we found that ATX mass (OR 1.06, 95% CI 1.03–1.10 per 10 ng mL⁻¹, P = 0.001) and ATX activity (OR 1.57, 95% CI 1.14–2.17 per 10 RFU min⁻¹, P = 0.005) were independently associated with CAVS. ATX activity interacted with Lp(a) (P = 0.004) and OxPL-apoB (P = 0.001) on CAVS risk. After adjustment, compared to patients with low ATX activity (dichotomized at the median value) and low Lp(a) (<50 mg dL⁻¹) or OxPL-apoB (<2.02 nmol L⁻¹, median) levels (referent), patients with both higher ATX activity (≥84 RFU min⁻¹) and Lp(a) (≥50 mg dL⁻¹) (OR 3.46, 95% CI 1.40–8.58, P = 0.007) or OxPL-apoB (≥2.02 nmol L⁻¹, median) (OR 5.48, 95% CI 2.45–12.27, P < 0.0001) had an elevated risk of CAVS.

Conclusion

Autotaxin is a novel and independent predictor of CAVS in patients with CAD.

from #Medicine via ola Kala on Inoreader http://ift.tt/24gyVqz
via IFTTT

Haploidentical Stem Cell Transplantation in Adult Haematological Malignancies

Haematopoietic stem cell transplantation is a well-established treatment option for both hematological malignancies and nonmalignant conditions such as aplastic anemia and haemoglobinopathies. For those patients lacking a suitable matched sibling or matched unrelated donor, haploidentical donors are an alternative expedient donor pool. Historically, haploidentical transplantation led to high rates of graft rejection and GVHD. Strategies to circumvent these issues include T cell depletion and management of complications thereof or T replete transplants with GVHD prophylaxis. This review is an overview of these strategies and contemporaneous outcomes for hematological malignancies in adult haploidentical stem cell transplant recipients.

from #Medicine via ola Kala on Inoreader http://ift.tt/1TQzvsu
via IFTTT

IJMS, Vol. 17, Pages 838: Hereditary Predispositions to Myelodysplastic Syndrome

Myelodysplastic syndromes (MDS) are heterogeneous clonal hematopoietic disorders characterized by ineffective hematopoiesis, bone marrow dysplasia, and peripheral cytopenias. Familial forms of MDS have traditionally been considered rare, especially in adults; however, the increasing availability of somatic and germline genetic analyses has identified multiple susceptibility loci. Bone marrow failure syndromes have been well-described in the pediatric setting, e.g., Fanconi anemia (FA), dyskeratosis congenita (DC), Diamond–Blackfan anemia (DBA), and Shwachman–Diamond syndrome (SBS), hallmarked by clinically-recognizable phenotypes (e.g., radial ray anomalies in FA) and significantly increased risks for MDS and/or acute myeloid leukemia (AML) in the setting of bone marrow failure. However, additional families with multiple cases of MDS or AML have long been reported in the medical literature with little known regarding potential hereditary etiologies. Over the last decade, genomic investigation of such families has revealed multiple genes conferring inherited risks for MDS and/or AML as the primary malignancy, including RUNX1, ANKRD26, DDX41, ETV6, GATA2, and SRP72. As these syndromes are increasingly appreciated in even apparently de novo presentations of MDS, it is important for hematologists/oncologists to become familiar with these newly-described syndromes. Herein, we provide a review of familial MDS syndromes and practical aspects of management in patients with predisposition syndromes.

from #Medicine via ola Kala on Inoreader http://ift.tt/25t7LPG
via IFTTT

Icariin Inhibits Pulmonary Hypertension Induced by Monocrotaline through Enhancement of NO/cGMP Signaling Pathway in Rats

It has been reported that icariin (ICA) increased contents of nitric oxide (NO) and cyclic guanosine monophosphate (cGMP) by improving expression of endothelial nitric oxide synthase (eNOS) and inhibition of phosphodiesterase type 5 (PDE5). In addition, dysfunction of the NO/cGMP pathway may play a crucial role in the pathogenesis of pulmonary hypertension (PH). In this study, the potential protective effects of ICA on PH induced by monocrotaline (MCT, 50 mg/kg) singly subcutaneous injection were investigated and the possible mechanisms involved in NO/cGMP pathway were explored in male Sprague Dawley rats. The results showed that ICA (20, 40, and 80 mg/kg/d) treatment by intragastric administration could significantly ameliorate PH and upregulate the expression of eNOS gene and downregulate the expression of PDE5 gene in MCT-treated rats. Both ICA (40 mg/kg/d) and L-arginine (200 mg/kg/d), a precursor of NO as positive control, notably increased the contents of NO and cGMP in lung tissue homogenate, which were inversed by treatment with -nitro-L-arginine-methyl ester (L-NAME), a NOS inhibitor, and L-NAME-treatment could also inhibit the protective effects of ICA (40 mg/kg/d) on mean pulmonary artery pressure and artery remodeling and tends to inhibit right ventricle hypertrophy index. In summary, ICA is effective in protecting against MCT-induced PH in rats through enhancement of NO/cGMP signaling pathway in rats.

from #Medicine via ola Kala on Inoreader http://ift.tt/25vEZ4B
via IFTTT

Sensors, Vol. 16, Pages 786: Adaptive Particle Filter for Nonparametric Estimation with Measurement Uncertainty in Wireless Sensor Networks

Particle filters (PFs) are widely used for nonlinear signal processing in wireless sensor networks (WSNs). However, the measurement uncertainty makes the WSN observations unreliable to the actual case and also degrades the estimation accuracy of the PFs. In addition to the algorithm design, few works focus on improving the likelihood calculation method, since it can be pre-assumed by a given distribution model. In this paper, we propose a novel PF method, which is based on a new likelihood fusion method for WSNs and can further improve the estimation performance. We firstly use a dynamic Gaussian model to describe the nonparametric features of the measurement uncertainty. Then, we propose a likelihood adaptation method that employs the prior information and a belief factor to reduce the measurement noise. The optimal belief factor is attained by deriving the minimum Kullback–Leibler divergence. The likelihood adaptation method can be integrated into any PFs, and we use our method to develop three versions of adaptive PFs for a target tracking system using wireless sensor network. The simulation and experimental results demonstrate that our likelihood adaptation method has greatly improved the estimation performance of PFs in a high noise environment. In addition, the adaptive PFs are highly adaptable to the environment without imposing computational complexity.

from #Medicine via ola Kala on Inoreader http://ift.tt/25t7igw
via IFTTT

Entropy, Vol. 18, Pages 211: A Confidence Set Analysis for Observed Samples: A Fuzzy Set Approach

Confidence sets are generally interpreted in terms of replications of an experiment. However, this interpretation is only valid before observing the sample. After observing the sample, any confidence sets have probability zero or one to contain the parameter value. In this paper, we provide a confidence set analysis for an observed sample based on fuzzy set theory by using the concept of membership functions. We show that the traditional ad hoc thresholds (the confidence and significance levels) can be attained from a general membership function. The applicability of the newly proposed theory is demonstrated by using well-known examples from the statistical literature and an application in the context of contingency tables.

from #Medicine via ola Kala on Inoreader http://ift.tt/25vGAHA
via IFTTT

IJMS, Vol. 17, Pages 838: Hereditary Predispositions to Myelodysplastic Syndrome

Energies, Vol. 9, Pages 423: Assessing Steady-State, Multivariate Experimental Data Using Gaussian Processes: The GPExp Open-Source Library

Experimental data are subject to different sources of disturbance and errors, whose importance should be assessed. The level of noise, the presence of outliers or a measure of the "explainability" of the key variables with respect to the externally-imposed operating condition are important indicators, but are not straightforward to obtain, especially if the data are sparse and multivariate. This paper proposes a methodology and a suite of tools implementing Gaussian processes for quality assessment of steady-state experimental data. The aim of the proposed tool is to: (1) provide a smooth (de-noised) multivariate operating map of the measured variable with respect to the inputs; (2) determine which inputs are relevant to predict a selected output; (3) provide a sensitivity analysis of the measured variables with respect to the inputs; (4) provide a measure of the accuracy (confidence intervals) for the prediction of the data; (5) detect the observations that are likely to be outliers. We show that Gaussian processes regression provides insightful numerical indicators for these purposes and that the obtained performance is higher or comparable to alternative modeling techniques. Finally, the datasets and tools developed in this work are provided within the GPExp open-source package.

from #Medicine via ola Kala on Inoreader http://ift.tt/1Uo8cpa
via IFTTT

Materials, Vol. 9, Pages 428: Using Central Composite Experimental Design to Optimize the Degradation of Tylosin from Aqueous Solution by Photo-Fenton Reaction

The feasibility of the application of the Photo-Fenton process in the treatment of aqueous solution contaminated by Tylosin antibiotic was evaluated. The Response Surface Methodology (RSM) based on Central Composite Design (CCD) was used to evaluate and optimize the effect of hydrogen peroxide, ferrous ion concentration and initial pH as independent variables on the total organic carbon (TOC) removal as the response function. The interaction effects and optimal parameters were obtained by using MODDE software. The significance of the independent variables and their interactions was tested by means of analysis of variance (ANOVA) with a 95% confidence level. Results show that the concentration of the ferrous ion and pH were the main parameters affecting TOC removal, while peroxide concentration had a slight effect on the reaction. The optimum operating conditions to achieve maximum TOC removal were determined. The model prediction for maximum TOC removal was compared to the experimental result at optimal operating conditions. A good agreement between the model prediction and experimental results confirms the soundness of the developed model.

from #Medicine via ola Kala on Inoreader http://ift.tt/1XIvQPY
via IFTTT

Sustainability, Vol. 8, Pages 524: Addressing the Security Concerns of Locals and Visitors for the Sustainable Development of Tourist Destinations

Tourism has long been recognized as a crime generator. This poses a dilemma in the sustainable development context: is continued tourist expansion sustainable if it generates increased law and order problems? Using the example of Brașov, Romania, this article considers the ways in which criminal justice agencies and the tourism sector have operated in partnership to ensure the security of both local residents and visitors. We argue that the success of the initiative depends on multi-agency working at the local level, but that the involvement of local residents is also crucial. This commitment may be tested as the nature of tourism changes. The research consists of an analysis of primary and secondary data. The results revealed that among the main security issues mentioned by tourists are not only robberies and other social and situational features that contributed to tourists feeling anxious or unsafe, but also the need to have access to good health services and easy access to money changing facilities, information centers, etc. Some improvements are suggested for the local Sustainable Development Strategy of Brașov.

from #Medicine via ola Kala on Inoreader http://ift.tt/1Uo7XdN
via IFTTT

Metals, Vol. 6, Pages 130: Vanadium Effect on a Medium Carbon Forging Steel

In the present work the influence of vanadium on the hardenability and the bainitic transformation of a medium carbon steel is analyzed. While V in solid solution enhances the former, it hardly affects bainitic transformation. The results also reveal an unexpected result, an increase of the prior austenite grain size as the V content increases.

from #Medicine via ola Kala on Inoreader http://ift.tt/1XIwhK7
via IFTTT

Entropy, Vol. 18, Pages 212: Extended First Law for Entanglement Entropy in Lovelock Gravity

The first law for the holographic entanglement entropy of spheres in a boundary CFT (Conformal Field Theory) with a bulk Lovelock dual is extended to include variations of the bulk Lovelock coupling constants. Such variations in the bulk correspond to perturbations within a family of boundary CFTs. The new contribution to the first law is found to be the product of the variation δ a of the "A"-type trace anomaly coefficient for even dimensional CFTs, or more generally its extension δ a * to include odd dimensional boundaries, times the ratio S / a * . Since a * is a measure of the number of degrees of freedom N per unit volume of the boundary CFT, this new term has the form μ δ N , where the chemical potential μ is given by the entanglement entropy per degree of freedom.

from #Medicine via ola Kala on Inoreader http://ift.tt/1Uo860N
via IFTTT

Molecules, Vol. 21, Pages 704: Significant Improvement of Metabolic Characteristics and Bioactivities of Clopidogrel and Analogs by Selective Deuteration

In the search for prodrug analogs of clopidogrel with improved metabolic characteristics and antiplatelet bioactivity, a group of clopidogrel and vicagrel analogs selectively deuterated at the benzylic methyl ester group were synthesized, characterized, and evaluated. The compounds included clopidogrel-d3 (8), 2-oxoclopidogrel-d3 (9), vicagrel-d3 (10a), and 12 vicagrel-d3 analogs (10b–10m) with different alkyl groups in the thiophene ester moiety. The D3C-O bond length in 10a was shown by X-ray single crystal diffraction to be shorter than the H3C-O bond length in clopidogrel, consistent with the slower rate of hydrolysis of 8 than of clopidogrel in rat whole blood in vitro. A study of the ability of the compounds to inhibit ADP-induced platelet aggregation in fresh rat whole blood collected 2 h after oral dosing of rats with the compounds (7.8 μmol/kg) showed that deuteration increased the activity of clopidogrel and that increasing the size of the alkyl group in the thiophene ester moiety reduced activity. A preliminary pharmacokinetic study comparing 10a with vicagrel administered simultaneously as single oral doses (72 μmol/kg of each drug) to male Wistar rats showed 10a generated more of its active metabolite than vicagrel. These results suggest that 10a is a potentially superior antiplatelet agent with improved metabolic characteristics and bioactivity, and less dose-related toxicity.

from #Medicine via ola Kala on Inoreader http://ift.tt/1XIvKYL
via IFTTT

Nutrients, Vol. 8, Pages 330: Iron, Anemia, and Iron Deficiency Anemia among Young Children in the United States

Iron deficiency and anemia are associated with impaired neurocognitive development and immune function in young children. Total body iron, calculated from serum ferritin and soluble transferrin receptor concentrations, and hemoglobin allow for monitoring of the iron and anemia status of children in the United States. The purpose of this analysis is to describe the prevalence of iron deficiency (ID), anemia, and iron deficiency anemia (IDA) among children 1–5 years using data from the 2007–2010 National Health and Nutrition Examination Survey (NHANES). Prevalence of ID, anemia, and IDA among children 1–5 years was 7.1% (5.5, 8.7), 3.2% (2.0, 4.3), and 1.1% (0.6, 1.7), respectively. The prevalence of both ID and anemia were higher among children 1–2 years (p < 0.05). In addition, 50% of anemic children 1–2 years were iron deficient. This analysis provides an update on the prevalence of ID, anemia, and IDA for a representative sample of US children. Our results suggest little change in these indicators over the past decade. Monitoring of ID and anemia is critical and prevention of ID in early childhood should remain a public health priority.

from #Medicine via ola Kala on Inoreader http://ift.tt/1Uo81u5
via IFTTT

Prepulse inhibition deficits in women with PTSD

Abstract

Prepulse inhibition (PPI) is an automatic and preattentive process, whereby a weak stimulus attenuates responding to a sudden and intense startle stimulus. PPI is a measure of sensorimotor filtering, which is conceptualized as a mechanism that facilitates processing of an initial stimulus and is protective from interruption by a later response. Impaired PPI has been found in (a) healthy women during the luteal phase of the menstrual cycle, and (b) individuals with types of psychopathology characterized by difficulty suppressing and filtering sensory, motor, or cognitive information. In the current study, 47 trauma-exposed women with or without posttraumatic stress disorder (PTSD) completed a PPI session during two different phases of the menstrual cycle: the early follicular phase, when estradiol and progesterone are both low, and the midluteal phase, when estradiol and progesterone are both high. Startle stimuli were 100 dB white noise bursts presented for 50 ms, and prepulses were 70 dB white noise bursts presented for 20 ms that preceded the startle stimuli by 120 ms. Women with PTSD showed deficits in PPI relative to the healthy trauma-exposed participants. Menstrual phase had no effect on PPI. These results provide empirical support for individuals with PTSD having difficulty with sensorimotor filtering. The potential utility of PPI as a Research Domain Criteria (RDoC) phenotype is discussed.

from #Medicine via ola Kala on Inoreader http://ift.tt/1WUpu0L
via IFTTT

Toxins, Vol. 8, Pages 164: Clinical Efficacy and Changes of Urothelial Dysfunction after Repeated Detrusor Botulinum Toxin A Injections in Chronic Spinal Cord-Injured Bladder

Chornic spinal cord injury (SCI) will induce bladder urothelium dysfunction. This study investigated the therapeutic effects on urothelial dysfunction after repeated detrusor injections of onabotulinumtoxinA (BoNT-A) in SCI patients with neurogenic detrusor overactivity (NDO). Twenty chronic suprasacral SCI patients with NDO were enrolled. The patients received 300 U BoNT-A injection into the detrusor every six months. The urothelium was assessed by cystoscopic biopsy at baseline and six months after each BoNT-A treatment. Immunofluorescence staining for urothelial dysfunction, including E-cadherin, zonula occludens-1 (ZO-1), tryptase for mast cell activity, and urothelial apoptosis were investigated. The outcome of urothelial dysfunction parameters after BoNT-A injection were compared between baseline and six months after each treatment. Repeated 300 U BoNT-A injections showed a sustained decrease of detrusor pressure compared with baseline. After three repeated BoNT-A detrusor injections, significantly greater distributions of E-cadherin (p = 0.042) and ZO-1 (p = 0.003) expressions, but no significant changes, of urothelial apoptosis and mast cell activation were found after repeated BoNT-A therapy. Urothelial dysfunction, such as adhesive and junction protein concentrations in SCI patients' bladders, recovered after three repeated cycles of BoNT-A treatment. The therapeutic effects sustained. However, urothelial inflammation and apoptosis after SCI were not significantly improved after three repeated BoNT-A injections.

from #Medicine via ola Kala on Inoreader http://ift.tt/1sGFmaz
via IFTTT

Metals, Vol. 6, Pages 131: Melt Protection of Mg-Al Based Alloys

This paper reports the current status of Mg melt protection in view to identify near-future challenges, but also opportunities, for Mg melt protection of Mg-Al based alloys. The goal is to design and manufacture sustainable Mg alloys for resource efficiency, recycling and minimising waste. Among alternative cover gas technologies for Mg melt protection other than SF6: commercially available technologies containing―HFC-134a, fluorinated ketone and dilute SO2―and developed technologies containing solid CO2, BF3 and SO2F2, can potentially produce toxic and/or corrosive by-products. On the other hand, additions of alkaline earth metal oxides to Mg and its alloys have developed a strong comparative advantage in the field of Mg melt protection. The near-future challenges and opportunities for Mg-Al based alloys include optimising and using CO2 gas as feedstock for both melt protection and grain refinement and TiO2 additions for melt protection.

from #Medicine via ola Kala on Inoreader http://ift.tt/25t0DTu
via IFTTT

Universe, Vol. 2, Pages 10: Reissner–Nordström Anti-de Sitter Black Holes in Mimetic F(R) Gravity

In this paper, we study under which conditions the Reissner–Nordström anti-de Sitter black hole can be a solution of the vacuum mimetic F ( R ) gravity with Lagrange multiplier and mimetic scalar potential. As the author demonstrates, the resulting picture in the mimetic F ( R ) gravity case is a trivial extension of the standard F ( R ) approach, and in effect, the metric perturbations in the mimetic F ( R ) gravity case, for the Reissner–Nordström anti-de Sitter black hole metric, at the first order of the perturbed variables are the same at the leading order.

from #Medicine via ola Kala on Inoreader http://ift.tt/1smcftf
via IFTTT

Sports, Vol. 4, Pages 31: Heart Rate Responses during Small Sided Games and Official Match-Play in Soccer

Small sided games (SSGs) are a match specific type of training. In addition, there is an insufficient number of studies that compare heart rate (HR) responses of SSGs and official match-play (OM). The purpose of this study was to investigate the heart rate responses during SSGs and OM in young soccer players. Twenty-two male soccer players (mean ± SD; age 17.4 ± 0.9 years, height 174.9 ± 6.6 cm, body weight 67.7 ± 8.1 kg) volunteered to participate in this study. The first session included anthropometric measurements and a maximum running test (RT). Following the RT session, all players participated in five different randomly ordered SSG sessions (3-, 4-, 5-, 7- and 9-a-side with goalkeepers). OMs were also monitored in the fourth week of the study. A one-way multivariate repeated-measures analysis of variance (MANOVA) was then conducted to evaluate the differences between the SSGs and OM. The results showed that 3-a-side elicited significantly higher HR and %HRmax than other SSGs and OM, whereas 9-a-side resulted in significantly lower HR and %HRmax compared to other SSG formats and OM (p < 0.05). In conclusion, 3-a-side, 4-a-side and 5-a-side SSG formats provide players with the opportunity to spend sufficient proportion of time spent in high intensity zones that are specific to match demands.

from #Medicine via ola Kala on Inoreader http://ift.tt/25t05x5
via IFTTT

Sustainability, Vol. 8, Pages 517: Evidence of Absolute Decoupling from Real World Policy Mixes in Europe

In resource economics, decoupling from environmental impacts is assumed to be beneficial. However, the success of efforts to increase resource productivity should be placed within the context of the earth's resources and ecosystems as theoretically finite and contingent on a number of threshold values. Thus far relatively few analyses exist of policies which have successfully implemented strategies for decoupling within these limits. Through ex-post evaluation of a number of real world policy mixes from European Union member states, this paper further develops definitions of the concept of decoupling. Beyond absolute (and relative) decoupling, "absolute decoupling within limits" is proposed as an appropriate term for defining resource-productivity at any scale which respects the existing real world limits on resources and ecosystems and as such, contributes to meeting sustainability objectives. Policy mixes presented here cover a range of resources such as fish stocks, fertilizers, aggregates and fossil based materials (plastics). Policy mixes demonstrating absolute decoupling and at least one where absolute decoupling within limits has occurred, provide insights on developing resource efficiency policies in Europe and beyond.

from #Medicine via ola Kala on Inoreader http://ift.tt/1smcnsS
via IFTTT

Materials, Vol. 9, Pages 427: Two Novel C3N4 Phases: Structural, Mechanical and Electronic Properties

We systematically studied the physical properties of a novel superhard (t-C3N4) and a novel hard (m-C3N4) C3N4 allotrope. Detailed theoretical studies of the structural properties, elastic properties, density of states, and mechanical properties of these two C3N4 phases were carried out using first-principles calculations. The calculated elastic constants and the hardness revealed that t-C3N4 is ultra-incompressible and superhard, with a high bulk modulus of 375 GPa and a high hardness of 80 GPa. m-C3N4 and t-C3N4 both exhibit large anisotropy with respect to Poisson's ratio, shear modulus, and Young's modulus. Moreover, m-C3N4 is a quasi-direct-bandgap semiconductor, with a band gap of 4.522 eV, and t-C3N4 is also a quasi-direct-band-gap semiconductor, with a band gap of 4.210 eV, with the HSE06 functional.

from #Medicine via ola Kala on Inoreader http://ift.tt/25t0IGT
via IFTTT

Energies, Vol. 9, Pages 424: A Multi-Component Additive to Improve the Thermal Stability of Li(Ni1/3Co1/3Mn1/3)O2-Based Lithium Ion Batteries

To improve the safety of lithium ion batteries, a multi-component (MC) additive (consisting of vinylene carbonate (VC), 1,3-propylene sulfite (PS) and dimethylacetamide (DMAC)) is used in the baseline electrolyte (1.0 M LiPF6/ethylene carbonate (EC) + diethyl carbonate (DEC)). The electrolyte with the MC additive is named safety electrolyte. The thermal stabilities of fully charged Li(Ni1/3Co1/3Mn1/3)O2 (NCM) mixed with the baseline electrolyte and safety electrolyte, respectively, are investigated using a C80 micro-calorimeter. The electrochemical performances of the NCM/baseline electrolyte/Li and NCM/safety electrolyte/Li half cells are evaluated using galvanostatic charge/discharge, cyclic voltammetry and alternating current (AC) impedance. The experimental results demonstrate that the fully charged NCM-safety electrolyte system releases less heat and reduces the main sharp exothermic peak value to a great extent, with a reduction of 40.6%. Moreover, the electrochemical performances of NCM/safety electrolyte/Li half cells are not worse, and are almost as good as that of the NCM/baseline electrolyte/Li half cells.

from #Medicine via ola Kala on Inoreader http://ift.tt/1smcN2b
via IFTTT

Universe, Vol. 2, Pages 9: Virial Theorem in Nonlocal Newtonian Gravity

Nonlocal gravity is the recent classical nonlocal generalization of Einstein's theory of gravitation in which the past history of the gravitational field is taken into account. In this theory, nonlocality appears to simulate dark matter. The virial theorem for the Newtonian regime of nonlocal gravity theory is derived and its consequences for "isolated" astronomical systems in virial equilibrium at the present epoch are investigated. In particular, for a sufficiently isolated nearby galaxy in virial equilibrium, the galaxy's baryonic diameter D 0 —namely, the diameter of the smallest sphere that completely surrounds the baryonic system at the present time—is predicted to be larger than the effective dark matter fraction f D M times a universal length that is the basic nonlocality length scale λ 0 ≈ 3 ± 2 kpc.

from #Medicine via ola Kala on Inoreader http://ift.tt/25t0Xl2
via IFTTT

Energies, Vol. 9, Pages 421: The Influence of Slight Protuberances in a Micro-Tube Reactor on Methane/Moist Air Catalytic Combustion

The combustion characteristics of methane/moist air in micro-tube reactors with different numbers and shapes of inner wall protuberances are investigated in this paper. The micro-reactor with one rectangular protuberance (six different sizes) was studied firstly, and it is shown that reactions near the protuberance are mainly controlled by diffusion, which has little effect on the outlet temperature and methane conversion rate. The formation of cavities and recirculation zones in the vicinity of protuberances leads to a significant increase of the Arrhenius reaction rate of CH4 and gas velocity. Next, among the six different simulated conditions (0–5 rectangular protuberances), the micro-tube reactor with five rectangular protuberances shows the highest methane conversion rate. Finally, the effect of protuberance shape on methane/moist air catalytic combustion is confirmed, and it is found that the protuberance shape has a greater influence on methane conversion rate than the number of protuberances. The methane conversion rate in the micro-tube decreases progressively in the following order: five triangular slight protuberances > five rectangular protuberances > five trapezoidal protuberances > smooth tube. In all tests of methane/moist air combustion conditions, the micro-tube with five triangular protuberances has the peak efficiency and is therefore recommended for high efficiency reactors.

from #Medicine via ola Kala on Inoreader http://ift.tt/1smcODt
via IFTTT

Energies, Vol. 9, Pages 418: Three-Dimensional Finite-Element Analysis of the Short-Time and Peak Withstand Current Tests in Substation Connectors

Power devices intended for high-voltage systems must be tested according to international standards, which includes the short-time withstand current test and peak withstand current test. However, these tests require very special facilities which consume huge amounts of electrical power. Therefore, mathematical tools to simulate such tests are highly appealing since they allow reproducing the electromagnetic and thermal behavior of the test object in a fast and economical manner. In this paper, a three-dimensional finite element method (3D-FEM) approach to simulate the transient thermal behavior of substation connectors is presented and validated against experimental data. To this end, a multiphysics 3D-FEM method is proposed, which considers both the connector and the reference power conductors. The transient and steady-state temperature profiles of both the conductors and connector provided by the 3D-FEM method prove its suitability and accuracy as compared to experimental data provided by short-circuit tests conducted in two high-current laboratories. The proposed simulation tool, which was proven to be accurate and realistic, may be particularly useful during the design and optimization phases of substation connectors since it allows anticipating the results of mandatory laboratory tests.

from #Medicine via ola Kala on Inoreader http://ift.tt/25t07F8
via IFTTT

Molecules, Vol. 21, Pages 694: The Effect of New Thiophene-Derived Aminophosphonic Derivatives on Growth of Terrestrial Plants: A Seedling Emergence and Growth Test

The aim of this work was to synthesize selected thiophene-derived aminophosphonic systems and evaluate the phytotoxicity of newly obtained products according to the OECD 208 Guideline. Seven new thiophene-derived N-substituted dimethyl aminomethylphosphonic acid esters 2a–h were synthesized by the addition of an appropriate phosphite to azomethine bond of starting Schiff bases 1a–h, and NMR spectroscopic properties of aminophosphonates were investigated. These eight compounds were analyzed in regard to their phytotoxicity towards two plants, radish (Raphanus sativus) and oat (Avena sativa). On the basis of the obtained results, it was found that tested aminophosphonates 2a–h showed an ecotoxicological impact against selected plants, albeit to various degrees.

from #Medicine via ola Kala on Inoreader http://ift.tt/1sGEDWW
via IFTTT

Computation, Vol. 4, Pages 21: Pore-Network Modeling of Water and Vapor Transport in the Micro Porous Layer and Gas Diffusion Layer of a Polymer Electrolyte Fuel Cell

In the cathode side of a polymer electrolyte fuel cell (PEFC), a micro porous layer (MPL) added between the catalyst layer (CL) and the gas diffusion layer (GDL) plays an important role in water management. In this work, by using both quasi-static and dynamic pore-network models, water and vapor transport in the MPL and GDL has been investigated. We illustrated how the MPL improved water management in the cathode. Furthermore, it was found that dynamic liquid water transport in the GDL was very sensitive to the built-up thermal gradient along the through-plane direction. Thus, we may control water vapor condensation only along GDL-land interfaces by properly adjusting the GDL thermal conductivity. Our numerical results can provide guidelines for optimizing GDL pore structures for good water management.

from #Medicine via ola Kala on Inoreader http://ift.tt/1NYjYaU
via IFTTT

Molecules, Vol. 21, Pages 701: Purification, Characterization and Biological Activity of Polysaccharides from Dendrobium officinale

Polysaccharide (DOPA) from the stem of D. officinale, as well as two fractions (DOPA-1 and DOPA-2) of it, were isolated and purified by DEAE cellulose-52 and Sephacryl S-300 chromatography, and their structural characteristics and bioactivities were investigated. The average molecular weights of DOPA-1 and DOPA-2 were 394 kDa and 362 kDa, respectively. They were mainly composed of d-mannose, d-glucose, and had a backbone consisting of 1,4-linked β-d-Manp and 1,4-linked β-d-Glcp with O-acetyl groups. Bioactivity studies indicated that both DOPA and its purified fractions (DOPA-1 and DOPA-2) could activate splenocytes and macrophages. The D. officinale polysaccharides had stimulatory effects on splenocytes, T-lymphocytes and B-lymphocytes, promoting the cell viability and NO production of RAW 264.7 macrophages. Furthermore, DOPA, DOPA-1 and DOPA-2 were found to protect RAW 264.7 macrophages against hydrogen peroxide (H2O2)-induced oxidative injury by promoting cell viability, suppressing apoptosis and ameliorating oxidative lesions. These results suggested that D. officinale polysaccharides possessed antioxidant activity and mild immunostimulatory activity.

from #Medicine via ola Kala on Inoreader http://ift.tt/1sGEUJg
via IFTTT

Polymers, Vol. 8, Pages 208: Synthesis of New Polyether Ether Ketone Derivatives with Silver Binding Site and Coordination Compounds of Their Monomers with Different Silver Salts

Polyether ether ketone (PEEK) is a well-known polymer used for implants and devices, especially spinal ones. To overcome the biomaterial related infection risks, 4-4′-difluorobenzophenone, the famous PEEK monomer, was modified in order to introduce binding sites for silver ions, which are well known for their antimicrobial activity. The complexation of these new monomers with different silver salts was studied. Crystal structures of different intermediates were obtained with a linear coordination between two pyridine groups and the silver ions in all cases. The mechanical and thermal properties of different new polymers were characterized. The synthesized PEEKN5 polymers showed similar properties than the PEEK ones whereas the PEEKN7 polymers showed similar thermal properties but the mechanical properties are not as good as the ones of PEEK. To improve these properties, these polymers were complexed with silver nitrate in order to "cross-link" with silver ions. The presence of ionic silver in the polymer was then confirmed by thermogravimetric analysis (TGA) and X-ray powder diffraction (XRPD). Finally, a silver-based antimicrobial compound was successfully coated on the surface of PEEKN5.

from #Medicine via ola Kala on Inoreader http://ift.tt/1NYjSzQ
via IFTTT

Molecules, Vol. 21, Pages 708: Antioxidative and Antidiabetic Effects of Natural Polyphenols and Isoflavones

Many polyphenols that contain more than two phenolic hydroxyl groups are natural antioxidants and can provide health benefits to humans. These polyphenols include, for example, oleuropein, hydroxytyrosol, catechin, chlorogenic acids, hesperidin, nobiletin, and isoflavones. These have been studied widely because of their strong radical-scavenging and antioxidative effects. These effects may contribute to the prevention of diseases, such as diabetes. Insulin secretion, insulin resistance, and homeostasis are important factors in the onset of diabetes, a disease that is associated with dysfunction of pancreatic β-cells. Oxidative stress is thought to contribute to this dysfunction and the effects of antioxidants on the pathogenesis of diabetes have, therefore, been investigated. Here, we summarize the antioxidative effects of polyphenols from the perspective of their radical-scavenging activities as well as their effects on signal transduction pathways. We also describe the preventative effects of polyphenols on diabetes by referring to recent studies including those reported by us. Appropriate analytical approaches for evaluating antioxidants in studies on the prevention of diabetes are comprehensively reviewed.

from #Medicine via ola Kala on Inoreader http://ift.tt/1sGEJ0E
via IFTTT

Polymers, Vol. 8, Pages 209: Demethylation of Wheat Straw Alkali Lignin for Application in Phenol Formaldehyde Adhesives

Lignin is a natural biopolymer with a complex three-dimensional network. It is the second most abundant natural polymer on earth. Commercially, lignin is largely obtained from the waste liquors of pulping and bioethanol productions. In this study, wheat straw alkali lignin (WSAL) was demethylated by using an in-situ generated Lewis acid under an optimized demethylation process. The demethylation process was monitored by a semi-quantitative Fourier Transform Infrared Spectroscopy (FTIR) method. The demethylated wheat straw alkali lignin (D-WSAL) was further characterized by Proton Nuclear Magnetic Resonance (1H NMR), Gel Permeation Chromatography (GPC), and titration methods. After the demethylation process, it was found that the relative value of the methoxy group decreased significantly from 0.82 to 0.17 and the phenolic hydroxyl group increased from 5.2% to 16.0%. Meanwhile, the hydroxyl content increased from 6.6% to 10.3%. GPC results suggested that the weighted averaged molecular weight of D-WSAL was lower than that of WSAL with a smaller polydispersity index. The D-WSAL was then used to replace 60 wt % of phenol to prepare lignin-based phenol formaldehyde adhesives (D-LPF). It was found that both the free formaldehyde content and the free phenol content in D-LPF were less than those of the lignin-based phenol formaldehyde adhesives without lignin demethylation (LPF). Gel time of D-LPF was shortened. Furthermore, the wet and dry bonding strengths of lap shear wood samples bonded using D-LPF were higher than those of the samples bonded using LPF. Therefore, D-WSAL has shown good potential for application in phenol formaldehyde adhesives.

from #Medicine via ola Kala on Inoreader http://ift.tt/1NYk02k
via IFTTT

Toxins, Vol. 8, Pages 164: Clinical Efficacy and Changes of Urothelial Dysfunction after Repeated Detrusor Botulinum Toxin A Injections in Chronic Spinal Cord-Injured Bladder

Geosciences, Vol. 6, Pages 26: Paleolimnology as a Tool to Achieve Environmental Sustainability in the Anthropocene: An Overview

Lacustrine sediment accumulation provides meaningful and diverse long-term records of environmental change. This overview highlights the usefulness of the paleolimnological approach in evaluating the magnitude and direction of human-induced environmental change in lakes and their catchments. Because of the services they provide, freshwater ecosystems have always been significantly affected by human activities. However, the rate and extent of human-induced change in continental freshwaters and their catchments has considerably increased since the beginning of industrialization (mid-18th century), and are even more pronounced since the advent of the "Great Acceleration" (since the mid-20th century). Global change, including climate and landscape changes, loss of biodiversity, species introductions and the spread of pollutants, leave traces in lake sediment archives that provide valuable long-term information with which to evaluate and quantify past environmental changes. This paper outlines how the knowledge gleaned from an interdisciplinary paleolimnological approach can benefit the development of mitigation and adaptation measures to current global change at various latitudes.

from #Medicine via ola Kala on Inoreader http://ift.tt/1NYk0PW
via IFTTT

JFMK, Vol. 1, Pages 240-248: Effects of Electrical Pulse and 6-DMAP on Cleavage of Golden Hamster Oocytes—Morphological and Phisiological Observations

The golden hamster is a well-established model system for studies of morphology, reproductive physiology, oncology, genetics and virology. The aim of this study was to establish experimental protocols necessary for cloning the golden hamster; we examined and optimized conditions for parthenogenesis and cleavage of its oocytes. We tested oocytes of different ages, including 15 h after Human chorionic gonadotropin (hCG), with two treatments: (1) an electrical pulse ranging from 10 to 600 V/mm and (2) incubation for 2 to 6 h in 2 mM 6-dimethylaminopurine (6-DMAP). These two conditions were tested both separately and in combination. We found that (i) in oocytes of different ages, cleavage exhibits a strength-dependent increase; (ii) 6-DMAP stimulates oocyte cleavage, but the cleavage rates are significantly low; and (iii) a combined treatment is more effective than a treatment with 6-DMAP alone, and is comparable to those achieved with high pulse stimuli. These results elucidate certain parameters important for the cloning of the golden hamster species.

from #Medicine via ola Kala on Inoreader http://ift.tt/1sGENNU
via IFTTT

Computers, Vol. 5, Pages 11: Continuity-Aware Scheduling Algorithm for Scalable Video Streaming

The consumer demand for retrieving and delivering visual content through consumer electronic devices has increased rapidly in recent years. The quality of video in packet networks is susceptible to certain traffic characteristics: average bandwidth availability, loss, delay and delay variation (jitter). This paper presents a scheduling algorithm that modifies the stream of scalable video to combat jitter. The algorithm provides unequal look-ahead by safeguarding the base layer (without the need for overhead) of the scalable video. The results of the experiments show that our scheduling algorithm reduces the number of frames with a violated deadline and significantly improves the continuity of the video stream without compromising the average Y Peek Signal-to-Noise Ratio (PSNR).

from #Medicine via ola Kala on Inoreader http://ift.tt/1NYk2HB
via IFTTT

Membranes, Vol. 6, Pages 30: Tethered and Polymer Supported Bilayer Lipid Membranes: Structure and Function

Solid supported bilayer lipid membranes are model systems to mimic natural cell membranes in order to understand structural and functional properties of such systems. The use of a model system allows for the use of a wide variety of analytical tools including atomic force microscopy, impedance spectroscopy, neutron reflectometry, and surface plasmon resonance spectroscopy. Among the large number of different types of model membranes polymer-supported and tethered lipid bilayers have been shown to be versatile and useful systems. Both systems consist of a lipid bilayer, which is de-coupled from an underlying support by a spacer cushion. Both systems will be reviewed, with an emphasis on the effect that the spacer moiety has on the bilayer properties.

from #Medicine via ola Kala on Inoreader http://ift.tt/1Pb9ZPR
via IFTTT

Molecules, Vol. 21, Pages 706: An Approach to Characterizing the Complicated Sequential Metabolism of Salidroside in Rats

Metabolic study of bioactive compounds that undergo a dynamic and sequential process of metabolism is still a great challenge. Salidroside, one of the most active ingredients of Rhodiola crenulata, can be metabolized in different sites before being absorbed into the systemic blood stream. This study proposed an approach for describing the sequential biotransformation process of salidroside based on comparative analysis. In vitro incubation, in situ closed-loop and in vivo blood sampling were used to determine the relative contribution of each site to the total metabolism of salidroside. The results showed that salidroside was stable in digestive juice, and it was metabolized primarily by the liver and the intestinal flora and to a lesser extent by the gut wall. The sequential metabolism method described in this study could be a general approach to characterizing the metabolic routes in the digestive system for natural products.

from #Medicine via ola Kala on Inoreader http://ift.tt/25sJcSY
via IFTTT

Molecules, Vol. 21, Pages 711: Molecular Genetic Characterization of an Anthrabenzoxocinones Gene Cluster in Streptomyces Sp. FJS31-2 for the Biosynthesis of BE-24566B and Zunyimycin Ale

Genome mining is an effective tool used to discover novel natural products from actinomycetes. Genome sequence analysis of Streptomyces sp. FJS31-2 revealed the presence of one putative type II polyketide gene cluster (ABX), which may correspond to type II polyketide products including BE-24566B and its chloro-derivatives. The addition of natural humus acid successfully activated the biosynthsis of the abx gene cluster. BE-24566B and its chloro-derivatives, named zunyimycin A, were also detected. The targeted deletion of the polyketide skeleton synthesis genes such as abxp, abxk, and abxs was performed in the wild strain to identify the gene cluster for BE-24566B biosynthesis.

from #Medicine via ola Kala on Inoreader http://ift.tt/1sWHwn8
via IFTTT

Minerals, Vol. 6, Pages 51: Preventive Replacement Decisions for Dragline Components Using Reliability Analysis

Reliability-based maintenance policies allow qualitative and quantitative evaluation of system downtimes via revealing main causes of breakdowns and discussing required preventive activities against failures. Application of preventive maintenance is especially important for mining machineries since production is highly affected from machinery breakdowns. Overburden stripping operations are one of the integral parts in surface coal mine productions. Draglines are extensively utilized in overburden stripping operations and they achieve earthmoving activities with bucket capacities up to 168 m3. The massive structure and operational severity of these machines increase the importance of performance awareness for individual working components. Research on draglines is rarely observed in the literature and maintenance studies for these earthmovers have been generally ignored. On this basis, this paper offered a comprehensive reliability assessment for two draglines currently operating in the Tunçbilek coal mine and discussed preventive replacement for wear-out components of the draglines considering cost factors.

from #Medicine via ola Kala on Inoreader http://ift.tt/25sIaGM
via IFTTT

Molecules, Vol. 21, Pages 683: Clematichinenoside (AR) Attenuates Hypoxia/Reoxygenation-Induced H9c2 Cardiomyocyte Apoptosis via a Mitochondria-Mediated Signaling Pathway

Mitochondria-mediated cardiomyocyte apoptosis is involved in myocardial ischemia/reperfusion (MI/R) injury. Clematichinenoside (AR) is a triterpenoid saponin isolated from the roots of Clematis chinensis with antioxidant and anti-inflammatory cardioprotection effects against MI/R injury, yet the anti-apoptotic effect and underlying mechanisms of AR in MI/R injury remain unclear. We hypothesize that AR may improve mitochondrial function to inhibit MI/R-induced cardiomyocyte apoptosis. In this study, we replicated an in vitro H9c2 cardiomyocyte MI/R model by hypoxia/reoxygenation (H/R) treatment. The viability of H9c2 cardiomyocytes was determined by MTT assay; apoptosis was evaluated by flow cytometry and TUNEL experiments; mitochondrial permeability transition pore (mPTP) opening was analyzed by a calcein-cobalt quenching method; and mitochondrial membrane potential (ΔΨm) was detected by JC-1. Moreover, we used western blots to determine the mitochondrial cytochrome c translocation to cytosolic and the expression of caspase-3, Bcl-2, and Bax proteins. These results showed that the application of AR decreased the ratio of apoptosis and the extent of mPTP opening, but increased ΔΨm. AR also inhibited H/R-induced release of mitochondrial cytochrome c and decreased the expression of the caspase-3, Bax proteins. Conversely, it remarkably increased the expression of Bcl-2 protein. Taken together, these results revealed that AR protects H9c2 cardiomyocytes against H/R-induced apoptosis through mitochondrial-mediated apoptotic signaling pathway.

from #Medicine via ola Kala on Inoreader http://ift.tt/1sWHmfr
via IFTTT

Recycling, Vol. 1, Pages 166-177: Parameters Affecting the Upcycling of Waste Cotton and PES/CO Textiles

Textile wastes in landfills are a major concern and offer wide scope in terms of waste management. The process of upcycling which aims at converting the waste into products of higher value is a feasible option. The research aims to explore factors to improve the process of upcycling. A set of mixed polyester/cotton (PES/CO) and cotton waste textiles from hospitals were examined for their properties. There are some physical parameters such as the mechanical properties and degree of polymerization that govern the process of upcycling. It was concluded that the textiles are degraded unevenly, so that it is difficult to predict their pattern of degradation. However, there are other possibilities and processes of using the waste textiles to reduce the waste in landfills.

from #Medicine via ola Kala on Inoreader http://ift.tt/25sIbdF
via IFTTT

Catalysts, Vol. 6, Pages 78: Solvent-Free Lipase-Catalyzed Synthesis of Technical-Grade Sugar Esters and Evaluation of Their Physicochemical and Bioactive Properties

Technical-grade oleic acid esters of sucrose and fructose were prepared using solvent-free biocatalysis at 65 °C, without any downstream purification applied, and their physicochemical and bioactivity-related properties were evaluated and compared to a commercially available sucrose laurate emulsifier. To increase the conversion of sucrose and fructose oleate, prepared previously using solvent-free lipase-catalyzed esterification catalyzed by Rhizomucor miehei lipase (81% and 83% ester, respectively), the enzymatic reaction conditions was continued using CaSO4 to control the reactor's air headspace and a lipase (from Candida antarctica B) with a hydrophobic immobilization matrix to provide an ultralow water activity, and high-pressure homogenation, to form metastable suspensions of 2.0–3.3 micron sized saccharide particles in liquid-phase reaction media. These measures led to increased ester content of 89% and 96% for reactions involving sucrose and fructose, respectively. The monoester content among the esters decreased from 90% to <70% due to differences in regioselectivity between the lipases. The resultant technical-grade sucrose and fructose lowered the surface tension to <30 mN/m, and possessed excellent emulsification capability and stability over 36 h using hexadecane and dodecane as oils, comparable to that of sucrose laurate and Tween® 80). The technical-grade sugar esters, particularly fructose oleate, more effectively inhibited gram-positive foodborne pathogens (Lactobacillus plantarum, Pediococcus pentosaceus and Bacillus subtilis). Furthermore, all three sugar esters displayed antitumor activity, particularly the two sucrose esters. This study demonstrates the importance of controlling the biocatalysts' water activity to achieve high conversion, the impact of a lipase's regioselectivity in dictating product distribution, and the use of solvent-free biocatalysis to important biobased surfactants useful in foods, cosmetics, personal care products, and medicine.

from #Medicine via ola Kala on Inoreader http://ift.tt/1sWHHPn
via IFTTT

Sensors, Vol. 16, Pages 780: Analytical Problems in Exposing Amperometric Enzyme Biosensors to Biological Fluids

Enzyme-based chemical biosensors are based on biological recognition. In order to operate, the enzymes must be available to catalyze a specific biochemical reaction and be stable under the normal operating conditions of the biosensor. Design of biosensors is based on knowledge about the target analyte, as well as the complexity of the matrix in which the analyte has to be quantified. This article reviews the problems resulting from the interaction of enzyme-based amperometric biosensors with complex biological matrices containing the target analyte(s). One of the most challenging disadvantages of amperometric enzyme-based biosensor detection is signal reduction from fouling agents and interference from chemicals present in the sample matrix. This article, therefore, investigates the principles of functioning of enzymatic biosensors, their analytical performance over time and the strategies used to optimize their performance. Moreover, the composition of biological fluids as a function of their interaction with biosensing will be presented.

from #Medicine via ola Kala on Inoreader http://ift.tt/22rPjFr
via IFTTT

Antimicrobial Susceptibility of Escherichia coli Strains Isolated from Alouatta spp. Feces to Essential Oils

This study evaluated the in vitro antibacterial activity of essential oils from Lippia graveolens (Mexican oregano), Origanum vulgaris (oregano), Thymus vulgaris (thyme), Rosmarinus officinalis (rosemary), Cymbopogon nardus (citronella), Cymbopogon citratus (lemongrass), and Eucalyptus citriodora (eucalyptus) against Escherichia coli () strains isolated from Alouatta spp. feces. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined for each isolate using the broth microdilution technique. Essential oils of Mexican oregano (MIC mean = 1818 μg mL−1; MBC mean = 2618 μg mL−1), thyme (MIC mean = 2618 μg mL−1; MBC mean = 2909 μg mL−1), and oregano (MIC mean = 3418 μg mL−1; MBC mean = 4800 μg mL−1) showed the best antibacterial activity, while essential oils of eucalyptus, rosemary, citronella, and lemongrass displayed no antibacterial activity at concentrations greater than or equal to 6400 μg mL−1. Our results confirm the antimicrobial potential of some essential oils, which deserve further research.

from #Medicine via ola Kala on Inoreader http://ift.tt/1NY764B
via IFTTT

Lubricants, Vol. 4, Pages 17: Assessing Boundary Film Forming Behavior of Phosphonium Ionic Liquids as Engine Lubricant Additives

The reduction of friction and wear losses in boundary lubrication regime of a piston ring-cylinder liner tribo-system has always been a challenge for engine and lubricant manufacturers. One way is to use lubricant additives, which can form boundary film quickly and reduce the direct contact between asperities. This article focuses on the assessment of boundary film forming behavior of two phosphonium-based ionic liquids (ILs) as additives in engine-aged lubricant to further improve its film forming capabilities and hence reduce friction and wear of contacting surfaces. A reciprocating piston ring segment-on-flat coupon under fully flooded lubrication conditions at room temperature (approx. 25 °C) was employed. The trihexyltetradecyl phosphonium bis(2-ethylhexyl) phosphate and trihexyltetradecyl phosphonium bis(2,4,4-tri-methylpentyl) phosphinate ionic liquids were used as additives in 6 vol. % quantity. Benchmark tests were conducted using fully formulated new lubricant of same grade (with and without ILs). Results revealed that the addition of phosphonium ILs to engine-aged lubricant led to quicker initiation of boundary film forming process. In addition, friction and wear performance of engine-aged lubricant improved by the addition of both ILs and these mixtures outperformed the fresh fully formulated oil. Chemical analysis showed higher concentration of phosphorus element on the worn surface indicating presence of ILs in the formed tribofilms.

from #Medicine via ola Kala on Inoreader http://ift.tt/1TQoVBM
via IFTTT

Energies, Vol. 9, Pages 417: Development of a PEM Fuel Cell City Bus with a Hierarchical Control System

The polymer electrolyte membrane (PEM) fuel cell system is considered to be an ideal alternative for the internal combustion engine, especially when used on a city bus. Hybrid buses with fuel cell systems and energy storage systems are now undergoing transit service demonstrations worldwide. A hybrid PEM fuel cell city bus with a hierarchical control system is studied in this paper. Firstly, the powertrain and hierarchical control structure is introduced. Secondly, the vehicle control strategy including start-stop strategy, energy management strategy, and fuel cell control strategy, including the hydrogen system and air system control strategies, are described in detail. Finally, the performance of the fuel cell was analyzed based on road test data. Results showed that the different subsystems were well-coordinated. Each component functioned in concert in order to ensure that both safety and speed requirements were satisfied. The output current of the fuel cell system changed slowly and the output voltage was limited to a certain range, thereby enhancing durability of the fuel cell. Furthermore, the economic performance was optimized by avoiding low load conditions.

from #Medicine via ola Kala on Inoreader http://ift.tt/1XI8c6f
via IFTTT

Algorithms, Vol. 9, Pages 37: A New Multi-Step Iterative Algorithm for Approximating Common Fixed Points of a Finite Family of Multi-Valued Bregman Relatively Nonexpansive Mappings

In this article, we introduce a new multi-step iteration for approximating a common fixed point of a finite class of multi-valued Bregman relatively nonexpansive mappings in the setting of reflexive Banach spaces. We prove a strong convergence theorem for the proposed iterative algorithm under certain hypotheses. Additionally, we also use our results for the solution of variational inequality problems and to find the zero points of maximal monotone operators. The theorems furnished in this work are new and well-established and generalize many well-known recent research works in this field.

from #Medicine via ola Kala on Inoreader http://ift.tt/1TQoSpv
via IFTTT

Beverages, Vol. 2, Pages 13: l-Theanine as a Functional Food Additive: Its Role in Disease Prevention and Health Promotion

Tea has been consumed for thousands of years and is an integral part of people's daily routine, as an everyday drink and a therapeutic aid for health promotion. Consumption of tea has been linked to a sense of relaxation commonly associated with the content of the non-proteinogenic amino acid theanine, which is found within the tea leaves. The aim of this review article is to outline the current methods for synthesis, extraction and purification of theanine, as well as to examine its potential benefits related to human health. These include improvements in cognitive and immune function, cancer prevention, reduced cardiovascular risk and its potential usefulness as a functional food product.

from #Medicine via ola Kala on Inoreader http://ift.tt/1XI8vOc
via IFTTT

Παρασκευή 27 Μαΐου 2016

Non-specific mechanisms in orthodox and CAM management of low back pain (MOCAM): theoretical framework and protocol for a prospective cohort study

Introduction

Components other than the active ingredients of treatment can have substantial effects on pain and disability. Such 'non-specific' components include: the therapeutic relationship, the healthcare environment, incidental treatment characteristics, patients' beliefs and practitioners' beliefs. This study aims to: identify the most powerful non-specific treatment components for low back pain (LBP), compare their effects on patient outcomes across orthodox (physiotherapy) and complementary (osteopathy, acupuncture) therapies, test which theoretically derived mechanistic pathways explain the effects of non-specific components and identify similarities and differences between the therapies on patient–practitioner interactions.

Methods and analysis

This research comprises a prospective questionnaire-based cohort study with a nested mixed-methods study. A minimum of 144 practitioners will be recruited from public and private sector settings (48 physiotherapists, 48 osteopaths and 48 acupuncturists). Practitioners are asked to recruit 10–30 patients each, by handing out invitation packs to adult patients presenting with a new episode of LBP. The planned multilevel analysis requires a final sample size of 690 patients to detect correlations between predictors, hypothesised mediators and the primary outcome (self-reported back-related disability on the Roland-Morris Disability Questionnaire). Practitioners and patients complete questionnaires measuring non-specific treatment components, mediators and outcomes at: baseline (time 1: after the first consultation for a new episode of LBP), during treatment (time 2: 2 weeks post-baseline) and short-term outcome (time 3: 3 months post-baseline). A randomly selected subsample of participants in the questionnaire study will be invited to take part in a nested mixed-methods study of patient–practitioner interactions. In the nested study, 63 consultations (21/therapy) will be audio-recorded and analysed quantitatively and qualitatively, to identify communication practices associated with patient outcomes.

Ethics and dissemination

The protocol is approved by the host institution's ethics committee and the NHS Health Research Authority Research Ethics Committee. Results will be disseminated via peer-reviewed journal articles, conferences and a stakeholder workshop.

from #Medicine via ola Kala on Inoreader http://ift.tt/1sTLq07
via IFTTT

The role of financial hardship, mastery and social support in the association between employment status and depression: results from an Australian longitudinal cohort study

Objective

There is robust epidemiological and clinical evidence of the harmful effects of unemployment on psychological well-being, but the mechanisms through which this occurs is still strongly debated. In addition, there is even less evidence on the impact of underemployment on mental health. Using longitudinal data collected from a cohort of 20–24 years old, the present study examines a range of employed states and investigates the role of mastery, financial hardship and social support in the relationship between labour status and depression.

Method

Responses were from the Personality and Total Health (PATH) Through Life Project: A representative, community-based survey conducted in Canberra and Queanbeyan (NSW) in Australia, where respondents (n=2404) in their early twenties were followed for 8 years. Depression was measured using the self-report Goldberg Depression Scale, with the likely presence of depression being indicated by scores 7 or greater.

Results

The analyses identified unemployment and underemployment as significant predictors of depression, compared to their employed counterparts. Both unemployment and underemployment remained significantly correlated with depression even after accounting for sociodemographic, economic and psychological variables. Social support, financial hardship and a sense of personal control (mastery) all emerged as important mediators between unemployment and depression.

Conclusions

Both unemployment and underemployment were associated with increased risk of depression. The strength of this relationship was attenuated but remained significant after accounting for key variables (mastery, financial hardship and social support), and extensive sociodemographic and health covariates, indicating that no or inadequate employment contributes to poorer mental health over and above these factors.

from #Medicine via ola Kala on Inoreader http://ift.tt/1sTLih1
via IFTTT

Catalysts, Vol. 6, Pages 80: Co-Assembled Supported Catalysts: Synthesis of Nano-Structured Supported Catalysts with Hierarchic Pores through Combined Flow and Radiation Induced Co-Assembled Nano-Reactors

A novel generic method of silica supported catalyst system generation from a fluid state is presented. The technique is based on the combined flow and radiation (such as microwave, thermal or UV) induced co-assembly of the support and catalyst precursors forming nano-reactors, followed by catalyst precursor decomposition. The transformation from the precursor to supported catalyst oxide state can be controlled from a few seconds to several minutes. The resulting nano-structured micro-porous silica supported catalyst system has a surface area approaching 300 m2/g and X-ray Diffraction (XRD)-based catalyst size controlled in the range of 1–10 nm in which the catalyst structure appears as lamellar sheets sandwiched between the catalyst support. These catalyst characteristics are dependent primarily on the processing history as well as the catalyst (Fe, Co and Ni studied) when the catalyst/support molar ratio is typically 0.1–2. In addition, Ca, Mn and Cu were used as co-catalysts with Fe and Co in the evaluation of the mechanism of catalyst generation. Based on extensive XRD, Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) studies, the micro- and nano-structure of the catalyst system were evaluated. It was found that the catalyst and silica support form extensive 0.6–2 nm thick lamellar sheets of 10–100 nm planar dimensions. In these lamellae, the alternate silica support and catalyst layer appear in the form of a bar-code structure. When these lamellae structures pack, they form the walls of a micro-porous catalyst system which typically has a density of 0.2 g/cm3. A tentative mechanism of catalyst nano-structure formation is provided based on the rheology and fluid mechanics of the catalyst/support precursor fluid as well as co-assembly nano-reactor formation during processing. In order to achieve these structures and characteristics, catalyst support must be in the form of silane coated silica nano-particles dispersed in water which also contains the catalyst precursor nitrate salt. This support-catalyst precursor fluid must have a sufficiently low viscosity but high elastic modulus (high extensional viscosity) to form films and bubbles when exposed to processing energy sources such as microwave, thermal, ultra-sound or UV-radiation or their combination. The micro-to-nano structures of the catalyst system are essentially formed at an early stage of energy input. It is shown that the primary particles of silica are transformed to a proto-silica particle state and form lamellar structures with the catalyst precursor. While the nano-structure is forming, water is evaporated leaving a highly porous solid support-catalyst precursor which then undergoes decomposition to form a silica-catalyst oxide system. The final catalyst system is obtained after catalyst oxide reduction. Although the XRD-based catalyst size changes slightly during the subsequent heat treatments, the nano-structure of the catalyst system remains substantially unaltered as evaluated through TEM images. However, if the catalyst preparation is carried out without film formation, the XRD-based catalyst size increases substantially by a factor of 2–8, with no significant alteration in surface area.

from #Medicine via ola Kala on Inoreader http://ift.tt/1NTndAp
via IFTTT

Symmetry, Vol. 8, Pages 38: On Solutions for Linear and Nonlinear Schrödinger Equations with Variable Coefficients: A Computational Approach

In this work, after reviewing two different ways to solve Riccati systems, we are able to present an extensive list of families of integrable nonlinear Schrödinger (NLS) equations with variable coefficients. Using Riccati equations and similarity transformations, we are able to reduce them to the standard NLS models. Consequently, we can construct bright-, dark- and Peregrine-type soliton solutions for NLS with variable coefficients. As an important application of solutions for the Riccati equation with parameters, by means of computer algebra systems, it is shown that the parameters change the dynamics of the solutions. Finally, we test numerical approximations for the inhomogeneous paraxial wave equation by the Crank-Nicolson scheme with analytical solutions found using Riccati systems. These solutions include oscillating laser beams and Laguerre and Gaussian beams.

from #Medicine via ola Kala on Inoreader http://ift.tt/1TKL18z
via IFTTT

Left ventricular hypertrophy: A rare cardiac involvement of Becker muscular dystrophy

Publication date: Available online 26 May 2016
Source:The Kaohsiung Journal of Medical Sciences
Author(s): Yu Kang, Yu-Cheng Chen, Qing Zhang

from #Medicine via ola Kala on Inoreader http://ift.tt/27W5M8S
via IFTTT

Selective Detection of RGD-Integrin Binding in Cancer Cells Using Tip Enhanced Raman Scattering Microscopy

TOC Graphic

Analytical Chemistry

DOI: 10.1021/acs.analchem.6b01344

from #Medicine via ola Kala on Inoreader http://ift.tt/1X1Whl5
via IFTTT

Enzyme-Immobilized 3D-Printed Reactors for Online Monitoring of Rat Brain Extracellular Glucose and Lactate

TOC Graphic

Analytical Chemistry

DOI: 10.1021/acs.analchem.6b00272

from #Medicine via ola Kala on Inoreader http://ift.tt/25nCzl3
via IFTTT

Exosomal miRNA Analysis in Non-small Cell Lung Cancer (NSCLC) Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

This protocol describes the feasibility to perform miRNA profiling in exosomes, released in plasma of NSCLC patients, through a commercial exosome isolation kit with Proteinase K and RNAse treatments, in order to avoid circulating miRNAs contamination and evaluate their biomarker features in NSCLC.

from #Medicine via ola Kala on Inoreader http://ift.tt/1UiNc3j
via IFTTT

Alkyne-Modulated Surface-Enhanced Raman Scattering-Palette for Optical Interference-Free and Multiplex Cellular Imaging

TOC Graphic

Analytical Chemistry

DOI: 10.1021/acs.analchem.6b01374

from #Medicine via ola Kala on Inoreader http://ift.tt/1qPqYLW
via IFTTT

Electrochemical Quantifying, Counting, and Sizing Supported Pt Nanoparticles in Real Time

TOC Graphic

Analytical Chemistry

DOI: 10.1021/acs.analchem.6b00966

from #Medicine via ola Kala on Inoreader http://ift.tt/1TNzRBq
via IFTTT

Αρχειοθήκη ιστολογίου

Αναζήτηση αυτού του ιστολογίου

Τρίτη 31 Μαΐου 2016

Graphical abstract

Abstract

Abstract

Δευτέρα 30 Μαΐου 2016

Abstract

Abstract

Background

Objective

Methods

Results

Conclusion

Abstract

Παρασκευή 27 Μαΐου 2016