OBJECTIVE: This study aims to investigate whether miR-98-5p can participate in the myocardial differentiation of bone marrow mesenchymal stem cells (MSCs) by regulating TBX5.
MATERIALS AND METHODS: In this study, we first identified the MSCs that were isolated from rat bone marrow samples. Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was performed to detect mRNA expressions of cardiac-related genes, including brain natriuretic peptide (BNP), α-actinin, and Islet-1. The binding site of miR-98-5p and TBX5 was detected by dual-luciferase report gene assay. In addition, co-transfection of miR-98-5p mimics and TBX5 overexpression plasmids was conducted to assess whether miR-98-5p could regulate myocardial differentiation by targeting TBX5.
RESULTS: Overexpression of TBX5 or knockdown of miR-98-5p promoted myocardial differentiation of BMSCs. The mRNA expressions of α-actinin and Islet-1 were significantly increased after the miR-98-5p knockdown. Dual-luciferase report gene assay showed that miR-98-5p could bind to TBX5, which was further verified by qRT-PCR. Additionally, TBX5 overexpression reversed the inhibitory effect of miR-98-5p on regulating the mRNA expressions of α-actinin and Islet-1.
CONCLUSIONS: MiR-98-5p can inhibit the differentiation of rat MSCs into cardiomyocytes through targeting TBX5.
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