Molecular weight, stiffness, temperature, and polymer and ionic concentrations are known to widely influence the viscosity of polymer solutions. Additionally, polymer molecular weight—which is related to its dimensions in solution—is one of its most important characteristics. In this communication, low molecular weight DNA from salmon sperm was purified and then studied in solutions in a wide concentration range (between 0.5 and 1600 mg/mL). The intrinsic viscosity of this low molecular weight DNA sample was firstly determined and the evidence of the overlap concentration was detected around the concentration of 125 mg/mL. The chain characteristics of these short molecules were studied in terms of the influence of their molecular weight on the solution viscosities and on the overlap parameter CDNA[η]. Furthermore, to complete previously reported experimental data, solutions of a large molecular weight DNA from calf-thymus were studied in a high concentration range (up to 40 mg/mL). The rheological behavior is discussed in terms of the generalized master curve obtained from the variation of the specific viscosity at zero shear rate (ηsp,0) as a function of CDNA[η].
http://ift.tt/2b27MEx
Τετάρτη 3 Αυγούστου 2016
Polymers, Vol. 8, Pages 279: Rheological Properties of DNA Molecules in Solution: Molecular Weight and Entanglement Influences
Symmetry, Vol. 8, Pages 75: Fuzzy System-Based Face Detection Robust to In-Plane Rotation Based on Symmetrical Characteristics of a Face
As face recognition technology has developed, it has become widely used in various applications such as door access control, intelligent surveillance, and mobile phone security. One of its applications is its adoption in TV environments to supply viewers with intelligent services and high convenience. In a TV environment, the in-plane rotation of a viewer's face frequently occurs because he or she may decide to watch the TV from a lying position, which degrades the accuracy of the face recognition. Nevertheless, there has been little previous research to deal with this problem. Therefore, we propose a new fuzzy system–based face detection algorithm that is robust to in-plane rotation based on the symmetrical characteristics of a face. Experimental results on two databases with one open database show that our method outperforms previous methods.
http://ift.tt/2ahy8FD
Sustainability, Vol. 8, Pages 740: Utilizing a Novel Approach at the Fuzzy Front-End of New Product Development: A Case Study in a Flexible Fabric Supercapacitor
The fuzzy front-end plays a most crucial part in new product development (NPD), leading to the success of product development and product launch in the market. This study proposes a novel method, TTRI_MP, by combining Crawford and Di Benedetto's model and Cooper's model, to strengthen the management of the fuzzy front-end. The proposed method comprises four stages: market exploration and technology forecasting, idea generation and segmentation, portfolio analysis and technology roadmapping (TRM). In the first stage, SWOT was utilized to identify the key strategic areas, and the technology readiness level (TRL) was adopted to position the level of developed technologies. In the second stage, the business concepts were required to go through the viability test and customers, collaborators, competitors and company (4C). In the third stage, the Strategic Position Analysis (SPAN) and Financial Analysis (FAN) developed by IBM were employed in the portfolio analysis to screen out potential NPD projects. In the last stage, the selected NPD projects were linked with their functions and technologies in the TRM chart. The method was successfully implemented by a research team working on a flexible fabric supercapacitor at the Taiwan Textile Research Institute (TTRI).
http://ift.tt/2b27KMV
IJERPH, Vol. 13, Pages 781: Physiological Effects of Nature Therapy: A Review of the Research in Japan
Humans have evolved into what they are today after the passage of 6–7 million years. If we define the beginning of urbanization as the rise of the industrial revolution, less than 0.01% of our species' history has been spent in modern surroundings. Humans have spent over 99.99% of their time living in the natural environment. The gap between the natural setting, for which our physiological functions are adapted, and the highly urbanized and artificial setting that we inhabit is a contributing cause of the "stress state" in modern people. In recent years, scientific evidence supporting the physiological effects of relaxation caused by natural stimuli has accumulated. This review aimed to objectively demonstrate the physiological effects of nature therapy. We have reviewed research in Japan related to the following: (1) the physiological effects of nature therapy, including those of forests, urban green space, plants, and wooden material and (2) the analyses of individual differences that arise therein. The search was conducted in the PubMed database using various keywords. We applied our inclusion/exclusion criteria and reviewed 52 articles. Scientific data assessing physiological indicators, such as brain activity, autonomic nervous activity, endocrine activity, and immune activity, are accumulating from field and laboratory experiments. We believe that nature therapy will play an increasingly important role in preventive medicine in the future.
http://ift.tt/2akORU3
Life, Vol. 6, Pages 31: AglM and VNG1048G, Two Haloarchaeal UDP-Glucose Dehydrogenases, Show Different Salt-Related Behaviors
Haloferax volcanii AglM and Halobacterium salinarum VNG1048G are UDP-glucose dehydrogenases involved in N-glycosylation in each species. Despite sharing >60% sequence identity and the ability of VNG1048G to functionally replace AglM in vivo, these proteins behaved differently as salinity changed. Whereas AglM was active in 2–4 M NaCl, VNG1048G lost much of its activity when salinity dropped below 3 M NaCl. To understand the molecular basis of this phenomenon, each protein was examined by size exclusion chromatrography in 2 M NaCl. Whereas AglM appeared as a dodecamer, VNG1048G was essentially detected as a dodecamer and a dimer. The specific activity of the VNG1048G dodecamer was only a sixth of that of AglM, while the dimer was inactive. As such, not only was the oligomeric status of VNG1048G affected by lowered salinity, so was the behavior of the individual dodecamer subunits. Analyzing surface-exposed residues in homology models of the two UDP-glucose dehydrogenases revealed the more acidic and less basic VNG1048G surface, further explaining the greater salt-dependence of the Hbt. salinarum enzyme.
http://ift.tt/2ahyjAC
Materials, Vol. 9, Pages 652: Fabricating Superior NiAl Bronze Components through Wire Arc Additive Manufacturing
Cast nickel aluminum bronze (NAB) alloy is widely used for large engineering components in marine applications due to its excellent mechanical properties and corrosion resistance. Casting porosity, as well as coarse microstructure, however, are accompanied by a decrease in mechanical properties of cast NAB components. Although heat treatment, friction stir processing, and fusion welding were implemented to eliminate porosity, improve mechanical properties, and refine the microstructure of as-cast metal, their applications are limited to either surface modification or component repair. Instead of traditional casting techniques, this study focuses on developing NAB components using recently expanded wire arc additive manufacturing (WAAM). Consumable welding wire is melted and deposited layer-by-layer on substrates producing near-net shaped NAB components. Additively-manufactured NAB components without post-processing are fully dense, and exhibit fine microstructure, as well as comparable mechanical properties, to as-cast NAB alloy. The effects of heat input from the welding process and post-weld-heat-treatment (PWHT) are shown to give uniform NAB alloys with superior mechanical properties revealing potential marine applications of the WAAM technique in NAB production.
http://ift.tt/2b28kut
Molecules, Vol. 21, Pages 1011: Struvite Precipitation as a Means of Recovering Nutrients and Mitigating Ammonia Toxicity in a Two-Stage Anaerobic Digester Treating Protein-Rich Feedstocks
Accumulation of ammonia, measured as total ammonia nitrogen (TAN), a product of protein decomposition in slaughterhouse wastes, inhibits the anaerobic digestion process, reducing digester productivity and leading to failure. Struvite precipitation (SP) is an effective means to remove TAN and enhance the buffering of substrates. Different Mg and P sources were evaluated as reactants in SP in acidogenic digester effluents to reduce its TAN levels. In order to measure impact of TAN removal, a standard biochemical methane potential (BMP) test was conducted to measure methane yield from treatments that had the highest TAN reductions. SP results showed 6 of 9 reagent combinations resulted in greater than 70% TAN removal. The BMP results indicated that SP treatment by adding Mg(OH)2 and H3PO4 resulted in 57.6% nitrogen recovery and 41.7% increase in methane yield relative to the substrate without SP. SP is an effective technology to improve nutrient recovery and methane production from the anaerobic digestion of protein-rich feedstocks.
http://ift.tt/2ahyj3H
JCM, Vol. 5, Pages 69: Comments on Bozzatello et al. Supplementation with Omega-3 Fatty Acids in Psychiatric Disorders: A Review of Literature Data. J. Clin. Med. 2016, 5, 67
Paola Bozzatello et al. [1] have done a comprehensive qualitative review of the potential use of long-chain polyunsaturated fatty acids in the prevention and treatment of mental disorders.[...]
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Therapeutic Strategies in HCC: Radiation Modalities
Patients with hepatocellular carcinoma (HCC) comply with an advanced disease and are not eligible for radical therapy. In this distressed scenario new treatment options hold great promise; among them transarterial chemoembolization (TACE) and transarterial metabolic radiotherapy (TAMR) have shown efficacy in terms of both tumor shrinking and survival. External radiation therapy (RTx) by using novel three-dimensional conformal radiotherapy has also been used for HCC patients with encouraging results while its role had been limited in the past for the low tolerance of surrounding healthy liver. The rationale of TAMR derives from the idea of delivering exceptional radiation dose locally to the tumor, with cell killing intent, while preserving normal liver from undue exposition and minimizing systemic irradiation. Since the therapeutic efficacy of TACE is being continuously disputed, the TAMR with 131I Lipiodol or 90Y microspheres has gained consideration providing adequate therapeutic responses regardless of few toxicities. The implementation of novel radioisotopes and technological innovations in the field of RTx constitutes an intriguing field of research with important translational aspects. Moreover, the combination of different therapeutic approaches including chemotherapy offers captivating perspectives. We present the role of the radiation-based therapies in hepatocellular carcinoma patients who are not entitled for radical treatment.
http://ift.tt/2aMOAxm
Detection of Extended Spectrum Beta-Lactamases Resistance Genes among Bacteria Isolated from Selected Drinking Water Distribution Channels in Southwestern Nigeria
Extended Spectrum Beta-Lactamases (ESBL) provide high level resistance to beta-lactam antibiotics among bacteria. In this study, previously described multidrug resistant bacteria from raw, treated, and municipal taps of DWDS from selected dams in southwestern Nigeria were assessed for the presence of ESBL resistance genes which include , , and by PCR amplification. A total of 164 bacteria spread across treated (33), raw (66), and municipal taps (68), belonging to -Proteobacteria, -Proteobacteria, -Proteobacteria, Flavobacteriia, Bacilli, and Actinobacteria group, were selected for this study. Among these bacteria, the most commonly observed resistance was for ampicillin and amoxicillin/clavulanic acid (61 isolates). Sixty-one isolates carried at least one of the targeted ESBL genes with being the most abundant (50/61) and being detected least (3/61). Klebsiella was the most frequently identified genus (18.03%) to harbour ESBL gene followed by Proteus (14.75%). Moreover, combinations of two ESBL genes, or , were observed in 11 and 1 isolate, respectively. In conclusion, classic ESBL gene was present in multiple bacterial strains that were isolated from DWDS sources in Nigeria. These environments may serve as foci exchange of genetic traits in a diversity of Gram-negative bacteria.
http://ift.tt/2aMO1DQ
The Midterm Surgical Outcome of Modified Expansive Open-Door Laminoplasty
Laminoplasty is a standard technique for treating patients with multilevel cervical spondylotic myelopathy. Modified expansive open-door laminoplasty (MEOLP) preserves the unilateral paraspinal musculature and nuchal ligament and prevents facet joint violation. The purpose of this study was to elucidate the midterm surgical outcomes of this less invasive technique. We retrospectively recruited 65 consecutive patients who underwent MEOLP at our institution in 2011 with at least 4 years of follow-up. Clinical conditions were evaluated by examining neck disability index, Japanese Orthopaedic Association (JOA), Nurick scale, and axial neck pain visual analog scale scores. Sagittal alignment of the cervical spine was assessed using serial lateral static and dynamic radiographs. Clinical and radiographic outcomes revealed significant recovery at the first postoperative year and still exhibited gradual improvement 1–4 years after surgery. The mean JOA recovery rate was 82.3% and 85% range of motion was observed at the final follow-up. None of the patients experienced aggravated or severe neck pain 1 year after surgery or showed complications of temporary C5 nerve palsy and lamina reclosure by the final follow-up. As a less invasive method for reducing surgical dissection by using various modifications, MEOLP yielded satisfactory midterm outcomes.
http://ift.tt/2aABL5R
Histopathological Analysis of PEEK Wear Particle Effects on the Synovial Tissue of Patients
Introduction. Increasing interest developed in the use of carbon-fiber-reinforced-poly-ether-ether-ketones (CFR-PEEK) as an alternative bearing material in knee arthroplasty. The effects of CFR-PEEK wear in in vitro and animal studies are controversially discussed, as there are no data available concerning human tissue. The aim of this study was to analyze human tissue containing CFR-PEEK as well as UHMWPE wear debris. The authors hypothesized no difference between the used biomaterials. Methods and Materials. In 10 patients during knee revision surgery of a rotating-hinge-knee-implant-design, synovial tissue samples were achieved (tibial inserts: UHMWPE; bushings and flanges: CFR-PEEK). One additional patient received revision surgery without any PEEK components as a control. The tissue was paraffin-embedded, sliced into 2 μm thick sections, and stained with hematoxylin and eosin in a standard process. A modified panoptical staining was also done. Results. A "wear-type" reaction was seen in the testing and the control group. In all samples, the UHMWPE particles were scattered in the tissue or incorporated in giant cells. CFR-PEEK particles were seen as conglomerates and only could be found next to vessels. CFR-PEEK particles showed no giant-cell reactions. In conclusion, the hypothesis has to be rejected. UHMWPE and PEEK showed a different scatter-behavior in human synovial tissue.
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Percutaneous irreversible electroporation for treatment of locally advanced pancreatic cancer following chemotherapy or radiochemotherapy
European Journal of Surgical Oncology
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Efficacy of sofosbuvir, velpatasvir, and GS-9857 in patients with genotype 1 hepatitis C virus infection in an open-label, phase 2 trial
Gastroenterology
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BMI, body fat mass and plasma leptin level in relation to cardiovascular diseases risk factors among adolescents in Taitung
Obesity Research & Clinical Practice
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Risks of bleeding recurrence and cardiovascular events with continued aspirin use after lower gastrointestinal hemorrhage
Gastroenterology
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Accuracy of resting metabolic rate prediction in overweight and obese Australian adults
Obesity Research & Clinical Practice
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Exercise-based Interventions for Nonalcoholic Fatty Liver Disease: A Meta-analysis and Meta-regression
Clinical Gastroenterology and Hepatology
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Adverse events after radiofrequency ablation in patients with Barrett’s esophagus: A systematic review and meta-analysis
Clinical Gastroenterology and Hepatology
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Role of bioactive fatty acids in nonalcoholic fatty liver disease
Nutrition Journal
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High or low intensity text-messaging combined with group treatment equally promote weight loss maintenance in obese adults
Obesity Research & Clinical Practice
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Stress reactivity in childhood functional abdominal pain or irritable bowel syndrome
European Journal of Pain
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Low serum vitamin D during remission increases risk of clinical relapse in patients with ulcerative colitis
Clinical Gastroenterology and Hepatology
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Clostridium difficile infection following systemic antibiotic administration in randomised controlled trials: A systematic review and meta-analysis
International Journal of Antimicrobial Agents
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Validation of data quality in the Swedish National Register for Oesophageal and Gastric Cancer
British Journal of Surgery
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Energies, Vol. 9, Pages 609: Real Time Hybrid Model Predictive Control for the Current Profile of the Tokamak à Configuration Variable (TCV)
Plasma stability is one of the obstacles in the path to the successful operation of fusion devices. Numerical control-oriented codes as it is the case of the widely accepted RZIp may be used within Tokamak simulations. The novelty of this article relies in the hierarchical development of a dynamic control loop. It is based on a current profile Model Predictive Control (MPC) algorithm within a multiloop structure, where a MPC is developed at each step so as to improve the Proportional Integral Derivative (PID) global scheme. The inner control loop is composed of a PID-based controller that acts over the Multiple Input Multiple Output (MIMO) system resulting from the RZIp plasma model of the Tokamak à Configuration Variable (TCV). The coefficients of this PID controller are initially tuned using an eigenmode reduction over the passive structure model. The control action corresponding to the state of interest is then optimized in the outer MPC loop. For the sake of comparison, both the traditionally used PID global controller as well as the multiloop enhanced MPC are applied to the same TCV shot. The results show that the proposed control algorithm presents a superior performance over the conventional PID algorithm in terms of convergence. Furthermore, this enhanced MPC algorithm contributes to extend the discharge length and to overcome the limited power availability restrictions that hinder the performance of advanced tokamaks.
http://ift.tt/2b1TZy6
Nanomaterials, Vol. 6, Pages 143: Distribution of Iron Oxide Core-Titanium Dioxide Shell Nanoparticles in VX2 Tumor Bearing Rabbits Introduced by Two Different Delivery Modalities
This work compares intravenous (IV) versus fluoroscopy-guided transarterial intra-catheter (IC) delivery of iron oxide core-titanium dioxide shell nanoparticles (NPs) in vivo in VX2 model of liver cancer in rabbits. NPs coated with glucose and decorated with a peptide sequence from cortactin were administered to animals with developed VX2 liver cancer. Two hours after NPs delivery tumors, normal liver, kidney, lung and spleen tissues were harvested and used for a series on histological and elemental analysis tests. Quantification of NPs in tissues was done both by bulk inductively coupled plasma mass spectrometry (ICP-MS) analysis and by hard X-ray fluorescence microscopy. Both IV and IC NPs injection are feasible modalities for delivering NPs to VX2 liver tumors with comparable tumor accumulation. It is possible that this is an outcome of the fact that VX2 tumors are highly vascularized and hemorrhagic, and therefore enhanced permeability and retention (EPR) plays the most significant role in accumulation of nanoparticles in tumor tissue. It is, however, interesting to note that IV delivery led to increased sequestration of NPs by spleen and normal liver tissue, while IC delivery lead to more NP positive Kupffer cells. This difference is most likely a direct outcome of blood flow dynamics. Armed with this knowledge about nanoparticle delivery, we plan to test them as radiosensitizers in the future.
http://ift.tt/2ahkaDM
Medical Sciences, Vol. 4, Pages 12: Novel Somatic Copy Number Alteration Identified for Cervical Cancer in the Mexican American Population
Cervical cancer affects millions of Americans, but the rate for cervical cancer in the Mexican American is approximately twice that for non-Mexican Americans. The etiologies of cervical cancer are still not fully understood. A number of somatic mutations, including several copy number alterations (CNAs), have been identified in the pathogenesis of cervical carcinomas in non-Mexican Americans. Thus, the purpose of this study was to investigate CNAs in association with cervical cancer in the Mexican American population. We conducted a pilot study of genome-wide CNA analysis using 2.5 million markers in four diagnostic groups: reference (n = 125), low grade dysplasia (cervical intraepithelial neoplasia (CIN)-I, n = 4), high grade dysplasia (CIN-II and -III, n = 5) and invasive carcinoma (squamous cell carcinoma (SCC), n = 5) followed by data analyses using Partek. We observed a statistically-significant difference of CNA burden between case and reference groups of different sizes (>100 kb, 10–100 kb and 1–10 kb) of CNAs that included deletions and amplifications, e.g., a statistically-significant difference of >100 kb deletions was observed between the reference (6.6%) and pre-cancer and cancer (91.3%) groups. Recurrent aberrations of 98 CNA regions were also identified in cases only. However, none of the CNAs have an impact on cancer progression. A total of 32 CNA regions identified contained tumor suppressor genes and oncogenes. Moreover, the pathway analysis revealed endometrial cancer and estrogen signaling pathways associated with this cancer (p < 0.05) using Kyoto Encyclopedia of Genes and Genomes (KEGG). This is the first report of CNAs identified for cervical cancer in the U.S. Latino population using high density markers. We are aware of the small sample size in the study. Thus, additional studies with a larger sample are needed to confirm the current findings.
http://ift.tt/2b1TYKy
Sensors, Vol. 16, Pages 1221: Wearable Sensors for eLearning of Manual Tasks: Using Forearm EMG in Hand Hygiene Training
In this paper, we propose a novel approach to eLearning that makes use of smart wearable sensors. Traditional eLearning supports the remote and mobile learning of mostly theoretical knowledge. Here we discuss the possibilities of eLearning to support the training of manual skills. We employ forearm armbands with inertial measurement units and surface electromyography sensors to detect and analyse the user's hand motions and evaluate their performance. Hand hygiene is chosen as the example activity, as it is a highly standardized manual task that is often not properly executed. The World Health Organization guidelines on hand hygiene are taken as a model of the optimal hygiene procedure, due to their algorithmic structure. Gesture recognition procedures based on artificial neural networks and hidden Markov modeling were developed, achieving recognition rates of 98 . 30 % ( ± 1 . 26 % ) for individual gestures. Our approach is shown to be promising for further research and application in the mobile eLearning of manual skills.
http://ift.tt/2ahk8Ma
Metabolites, Vol. 6, Pages 24: Impact of Glucocorticoid Excess on Glucose Tolerance: Clinical and Preclinical Evidence
Glucocorticoids (GCs) are steroid hormones that exert important physiological actions on metabolism. Given that GCs also exert potent immunosuppressive and anti-inflammatory actions, synthetic GCs such as prednisolone and dexamethasone were developed for the treatment of autoimmune- and inflammatory-related diseases. The synthetic GCs are undoubtedly efficient in terms of their therapeutic effects, but are accompanied by significant adverse effects on metabolism, specifically glucose metabolism. Glucose intolerance and reductions in insulin sensitivity are among the major concerns related to GC metabolic side effects, which may ultimately progress to type 2 diabetes mellitus. A number of pre-clinical and clinical studies have aimed to understand the repercussions of GCs on glucose metabolism and the possible mechanisms of GC action. This review intends to summarize the main alterations that occur in liver, skeletal muscle, adipose tissue, and pancreatic islets in the context of GC-induced glucose intolerance. For this, both experimental (animals) and clinical studies were selected and, whenever possible, the main cellular mechanisms involved in such GC-side effects were discussed.
http://ift.tt/2b1TXpY
Cafeteria diet induce changes in blood flow that are more related with heat dissipation than energy accretion
Background. A "cafeteria" diet is a self-selected high-fat diet, providing an excess of energy, which can induce obesity. Excess of lipids in the diet hampers glucose utilization eliciting insulin resistance, which, further limits amino acid oxidation for energy. Methods. Male Wistar rats were exposed for a month to "cafeteria" diet. Rats were cannulated and fluorescent microspheres were used to determine blood flow. Results. Exposure to the cafeteria diet did not change cardiac output, but there was a marked shift in organ irrigation. Skin blood flow decreased to compensate increases in lungs and heart. Blood flow through adipose tissue tended to increase in relation to controls, but was considerably increased in brown adipose tissue (on a weight basis). Discussion. The results suggest that the cafeteria diet-induced changes were related to heat transfer and disposal.
http://ift.tt/2aSjO46
A multifunctional GH39 glycoside hydrolase from the anaerobic gut fungus Orpinomyces sp. strain C1A
http://ift.tt/2aQnaYS
The pes of Australovenator wintonensis (Theropoda: Megaraptoridae): analysis of the pedal range of motion and biological restoration
http://ift.tt/2aQmB1d
Altered corticospinal function during movement preparation in humans with spinal cord injury
Abstract
Corticospinal output is modulated in a task-dependent manner during the preparatory phase of upcoming movements in humans. Whether this ability is preserved after spinal cord injury (SCI) is unknown. In this study, we examined motor evoked potentials elicited by cortical (MEPs) and subcortical (CMEPs) stimulation of corticospinal axons and short-interval intracortical inhibition in the first dorsal interosseous muscle in the preparatory phase of a reaction-time task where individuals with chronic incomplete cervical SCI and age-matched controls needed to suppress (NOGO) or initiate (GO) ballistic index finger isometric voluntary contractions. Reaction times were prolonged in SCI participants compared with control subjects and stimulation was provided ∼90 ms prior to movement onset in each group. During NOGO trials, both MEPs and CMEPs remained unchanged compared to baseline in SCI participants but were suppressed in control subjects. Notably, during GO trials, MEPs increased to a similar extent in both groups but CMEPs increased only in controls. The magnitude of short-interval intracortical inhibition increased in controls but not in SCI subjects during NOGO trials and decreased in both groups in GO trials. These novel observations reveal that humans with incomplete cervical SCI have an altered ability to modulate corticospinal excitability during movement preparation when there is a need to suppress but not to execute upcoming rapid finger movements, which is likely related to impaired transmission at a cortical and spinal level. Thus, deficits in corticospinal transmission after human SCI extend to the preparatory phase of upcoming movements.
This article is protected by copyright. All rights reserved
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Future Internet, Vol. 8, Pages 39: Ontology-Based Representation and Reasoning in Building Construction Cost Estimation in China
Cost estimation is one of the most critical tasks for building construction project management. The existing building construction cost estimation methods of many countries, including China, require information from several sources, including material, labor, and equipment, and tend to be manual, time-consuming, and error-prone. To solve these problems, a building construction cost estimation model based on ontology representation and reasoning is established, which includes three major components, i.e., concept model ontology, work item ontology, and construction condition ontology. Using this model, the cost estimation information is modeled into OWL axioms and SWRL rules that leverage the semantically rich ontology representation to reason about cost estimation. Based on OWL axioms and SWRL rules, the cost estimation information can be translated into a set of concept models, work items, and construction conditions associated with the specific construction conditions. The proposed method is demonstrated in Protégé 3.4.8 through case studies based on the Measurement Specifications of Building Construction and Decoration Engineering taken from GB 50500-2013 (the Chinese national mandatory specifications). Finally, this research discusses the limitations of the proposed method and future research directions. The proposed method can help a building construction cost estimator extract information more easily and quickly.
http://ift.tt/2aQmjYg
The Natural Occurring Compounds Targeting Endoplasmic Reticulum Stress
ER stress has been implicated in pathophysiological development of many diseases. Persistent overwhelming stimuli trigger ER stress to initiate apoptosis, autophagy, and cell death. IRE1-JNK and eIF2α-CHOP signaling pathways are the two important players of ER stress, which is also modulated by ROS production, calcium disturbance, and inflammatory factors. ER stress has been developed as a novel strategy for diseases management. Recently, a vast of research focuses on the natural occurring compounds targeting ER stress, which results in medical benefits to human diseases. These small reported molecules mainly include polyphenols, alkaloids, and saponins. Many of them have been developed for use in clinical applications. To better understand the pharmacological mechanism of these molecules in ER stress in diseases, efforts have been made to discover and deliver medical merits. In this paper, we will summarize the natural occurring compounds targeting ER stress.
http://ift.tt/2atOFYa
Influence of Temperature on Free Radical Generation in Propolis-Containing Ointments
Free radicals thermally generated in the ointments containing propolis were studied by electron paramagnetic resonance (EPR) spectroscopy. The influence of temperature on the free radical concentration in the propolis ointments was examined. Two ointment samples with different contents of propolis (5 and 7%, resp.) heated at temperatures of 30°C, 40°C, 50°C, and 60°C, for 30 min., were tested. Homogeneously broadened EPR lines and fast spin-lattice interactions characterized all the tested samples. Free radicals concentrations in the propolis samples ranged from 1018 to 1020 spin/g and were found to grow in both propolis-containing ointments along with the increasing heating temperature. Free radical concentrations in the ointments containing 5% and 7% of propolis, respectively, heated at temperatures of 30°C, 40°C, and 50°C were only slightly different. Thermal treatment at the temperature of 60°C resulted in a considerably higher free radical formation in the sample containing 7% of propolis when related to the sample with 5% of that compound. The EPR examination indicated that the propolis ointments should not be stored at temperatures of 40°C, 50°C, and 60°C. Low free radical formation at the lowest tested temperatures pointed out that both examined propolis ointments may be safely stored up to the temperature of 30°C.
http://ift.tt/2aHObLd
Animals, Vol. 6, Pages 47: Using the Horse Grimace Scale (HGS) to Assess Pain Associated with Acute Laminitis in Horses (Equus caballus)
Acute laminitis is a common equine disease characterized by intense foot pain, both acutely and chronically. The Obel grading system is the most widely accepted method for describing the severity of laminitis by equine practitioners, however this method requires movement (walk and trot) of the horse, causing further intense pain. The recently developed Horse Grimace Scale (HGS), a facial-expression-based pain coding system, may offer a more effective means of assessing the pain associated with acute laminitis. The aims of this study were: to investigate whether HGS can be usefully applied to assess pain associated with acute laminitis in horses at rest, and to examine if scoring HGS using videos produced similar results as those obtained from still images. Ten horses, referred as acute laminitis cases with no prior treatment, were included in the study. Each horse was assessed using the Obel and HGS (from images and videos) scales: at the admission (before any treatment) and at seven days after the initial evaluation and treatment. The results of this study suggest that HGS is a potentially effective method to assess pain associated with acute laminitis in horses at rest, as horses showing high HGS scores also exhibited higher Obel scores and veterinarians classified them in a more severe painful state. Furthermore, the inter-observer reliability of the HGS total score was good for both still images and video evaluation. There was no significant difference in HGS total scores between the still images and videos, suggesting that there is a possibility of applying the HGS in clinical practice, by observing the horse for a short time. However, further validation studies are needed prior to applying the HGS in a clinical setting.
http://ift.tt/2aHJ4KP
Water, Vol. 8, Pages 326: Assessment of the Impact of Subsurface Agricultural Drainage on Soil Water Storage and Flows of a Small Watershed
3D hydrological modeling was performed, using CATHY (acronym for CATchment HYdrology model), with the basic objective of checking whether the model could reproduce the effects of subsurface agricultural drainage on stream flows and soil water storage. The model was also used to further our understanding of the impact of soil hydrodynamic properties on watershed hydrology. Flows simulated by CATHY were consistent with traditional subsurface drainage approaches and, for wet years, flows at the outlet of the study watershed corroborated well with observed data. Temporal storage variation analyses illustrated that flows depended not only on the amount of rainfall, but also on its distribution throughout the year. Subsurface agricultural drainage increased base and total flows, and decreased peak flows. Hydrograph separation using simulated results indicated that exfiltration was the most dominant process; peak flows were largely characterized by overland flow; and subsurface drain flow variations were low.
http://ift.tt/2atJiZb
Animals, Vol. 6, Pages 46: Quantity Discrimination in Domestic Rats, Rattus norvegicus
Quantity discrimination is a basic form of numerical competence where an animal distinguishes which of two amounts is greater in size. Whilst quantity discrimination in rats has been investigated via training paradigms, rats' natural quantity discrimination abilities without explicit training for a desired response have not been explored. This study investigated domestic rats' ability to perform quantity discrimination. Domestic rats ( n = 12) were examined for their ability to distinguish the larger amount under nine quantity comparisons. One-sample t -tests identified a significant preference for the larger quantity in comparisons of 1 vs. 2, 2 vs. 3, 3 vs. 5, 3 vs. 8, 4 vs. 6, and 4 vs. 8. No preference between quantities was found for comparisons of 3 vs. 4, 4 vs. 5 and 5 vs. 6. Overall, this study drew two key conclusions. Firstly, that domestic rats are capable of performing quantity discrimination without extensive training. Secondly, as subjects adhered to Weber's law, it was concluded that the approximate number system underpins domestic rats' ability to perform spontaneous quantity discrimination.
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IJERPH, Vol. 13, Pages 779: Depression, Anxiety and Symptoms of Stress among Baccalaureate Nursing Students in Hong Kong: A Cross-Sectional Study
This study examines the prevalence of depression, anxiety and symptoms of stress among baccalaureate nursing students in Hong Kong. Recent epidemiological data suggest that the prevalence of mild to severe depression, anxiety and stress among qualified nurses in Hong Kong stands at 35.8%, 37.3% and 41.1%, respectively. A total of 661 nursing students were recruited to participate in our cross-sectional mental health survey using the Depression, Anxiety and Stress Scale 21. Multiple logistic regression was used to determine significant relationships between variables. Working in general medicine, being in financial difficulty, having sleep problems, not having leisure activity and perceiving oneself in poor mental health were significant correlates of past-week depression, anxiety and stress. Year of study, physical inactivity and family crisis in the past year correlated significantly with depression. Imbalanced diets significantly correlated with anxiety. Stress was significantly associated with a lack of alone time. This is the first study to confirm empirically that clinical specialty, financial difficulties and lifestyle factors can increase nursing students' levels of depression and anxiety and symptoms of stress. Prevention, including the early detection and treatment of mental disorder, promises to reduce the prevalence of these indicators among this group.
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Energies, Vol. 9, Pages 611: Environment-Friendly Heterogeneous Alkaline-Based Mixed Metal Oxide Catalysts for Biodiesel Production
The critical problem arising from the depletion of fossil fuels has stimulated recent interests in alternative sources for petroleum-based fuel. An alternative fuel should be technically feasible, readily available, sustainable, and techno-economically competitive. Biodiesel is considered as a potential replacement of conventional diesel fuel, which is prepared from non-edible and high-acid feedstock via transesterification technology. The focus of this study is to investigate the catalytic activity of mixed metal oxides (MMOs) as catalysts for biodiesel production by using non-edible jatropha oil as feedstock. Various types of MMOs (CaO-MgO, CaO-ZnO, CaO-La2O3, and MgO-ZnO) were synthesized via a co-precipitation method. In this study, transesterification activities are closely related to the physicochemical properties of catalysts. The presence of different active metals in the binary system greatly influenced the surface area, basicity, and the stability of catalysts. The catalytic activity of MMO catalysts was increased in the order of CaO-ZnO (94% ± 1%) > CaO ~ CaO-MgO ~ CaO-La2O3 (~90% ± 2%) > MgO-ZnO (83% ± 2%) > MgO (64% ± 1%) > ZnO (41% ± 2%) > La2O3 (23% ± 1%). In addition, the MMO catalysts, especially CaO-ZnO, demonstrated high reusability and catalyst stability for four cycles of transesterification reaction of jatropha oil.
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Reduced activity of an important enzyme identified among suicidal patients
It is known that people who have attempted suicide have ongoing inflammation in their blood and spinal fluid. Now, a collaborative study from research teams in Sweden, the US and Australia published in Translational Psychiatry shows that suicidal patients have a reduced activity of an enzyme that regulates inflammation and its byproducts. (English text: edited and translated by Van Andel Research Institute). The study is the result of a longstanding partnership between the research teams of Professor Sophie Erhardt, Karolinska Institutet, Professor Lena Brundin at Van Andel Research Institute in Grand Rapids, USA, and Professor Gilles Guillemin at Macquarie University in Australia. The overall aim of the research is to find ways to identify suicidal patients. Biological factors "Currently, there are no biomarkers for psychiatric illness, namely biological factors that can be measured and provide information about the patient's psychiatric health. If a simple blood test can identify individuals at risk of taking their lives, that would be a huge step forward", said Sophie Erhardt, a Professor at the Department of Physiology and Pharmacology at the Karolinska Institutet, who led the work along with Lena Brundin. The researchers analyzed certain metabolites, byproducts formed during infection and inflammation, in the blood and cerebrospinal fluid from patients who tried to take their own lives. Previously it has been shown that such patients have ongoing inflammation in the blood and cerebrospinal fluid. This new work has succeeded in showing that patients who have attempted suicide have reduced activity of an enzyme called ACMSD, which regulates inflammation and its byproducts. "We believe that people who have reduced activity of the enzyme are especially vulnerable to developing depression and suicidal tendencies when they suffer from various infections or inflammation. We also believe that inflammation is likely to easily become chronic in people with impaired activity of ACMSD," said Brundin Important balance The substance that the enzyme ACMSD produces, picolinic acid, is greatly reduced in both plasma and in the spinal fluid of suicidal patients. Another product, called quinolinic acid, is increased. Quinolinic acid is inflammatory and binds to and activates glutamate receptors in the brain. Normally, ACMSD produces picolinic acid at the expense of quinolinic acid, thus maintaining an important balance. "We now want to find out if these changes are only seen in individuals with suicidal thoughts or if patients with severe depression also exhibit this. We also want to develop drugs that might activate the enzyme ACMSD and thus restore the balance between quinolinic and picolinic acid," Erhardt said. The study was funded with the support of the Swedish Research Council, Region Skåne and Central ALF funds. Additional support came from National Institute of Mental Health (NIMH), the American Foundation for Suicide Prevention, Van Andel Research Institute, Rocky Mountain MIRECC, the Merit Review CSR & D and the Joint Institute for Food Safety and Applied Nutrition (University of Maryland), and the Australian Research Council. Several of the researchers have indicated that they have business interests, which are recognized in the article. Publication An enzyme in the kynurenine pathway that governs vulnerability to suicidal behavior by regulating excitotoxicity and neuroinflammation Lena Brundin, Carl M. Sellgren, Chai K. Lim, Jamie Grit, Erik Palsson, Mikael Landen, Martin Samuelsson, Christina Lundgren, Patrik Brundin, Dietmar Fuchs, Teodor T. Postolache, Lil Träskman-Bendz, Gilles J. Guillemin, Sophie Erhardt. Translational Psychiatry, published online August 2, 2016, doi: 10.1038 / TP.2016.133.
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Viruses, Vol. 8, Pages 213: Binding Specificities of the Telomere Phage ϕKO2 Prophage Repressor CB and Lytic Repressor Cro
Temperate bacteriophages possess a genetic switch which regulates the lytic and lysogenic cycle. The genomes of the temperate telomere phages N15, PY54, and ϕKO2 harbor a primary immunity region (immB) comprising genes for the prophage repressor (cI or cB), the lytic repressor (cro) and a putative antiterminator (q). The roles of these products are thought to be similar to those of the lambda proteins CI (CI prophage repressor), Cro (Cro repressor), and Q (antiterminator Q), respectively. Moreover, the gene order and the location of several operator sites in the prototype telomere phage N15 and in ϕKO2 are reminiscent of lambda-like phages. We determined binding sites of the ϕKO2 prophage repressor CB and lytic repressor Cro on the ϕKO2 genome in detail by electrophoretic mobility shift assay (EMSA) studies. Unexpectedly, ϕKO2 CB and Cro revealed different binding specificities. CB was bound to three OR operators in the intergenic region between cB and cro, two OL operators between cB and the replication gene repA and even to operators of N15. Cro bound exclusively to the 16 bp operator site OR3 upstream of the ϕKO2 prophage repressor gene. The ϕKO2 genes cB and cro are regulated by several strong promoters overlapping with the OR operators. The data suggest that Cro represses cB transcription but not its own synthesis, as already reported for PY54 Cro. Thus, not only PY54, but also phage ϕKO2 possesses a genetic switch that diverges significantly from the switch of lambda-like phages.
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The effects of different types of ankle-foot orthoses on postural responses in individuals with walking impairments.
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Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
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Alimentary Pharmacology &Therapeutics, EarlyView. https://ift.tt/2qECBIJ
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heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
