Erzhi Formula Extracts Reverse Renal Injury in Diabetic Nephropathy Rats by Protecting the Renal Podocytes

Podocytes injury was a crucial factor resulting in diabetic nephropathy (DN). Erzhi formula extract (EZF) was a clinical effective Chinese medicine on DN, but its mechanism was unclear. In this study, the main compounds of EZF and their pharmacokinetics in rat were detected by HPLC-MS/MS. And then, blood glucose, urine protein, renal index, renal microstructural (HE/PAS staining), inflammatory factors (IL-β, TNF-α, IL-6), and protein/mRNA expression related to the function of podocyte (CD2AP and Podocin) in DN rats were investigated after the oral administration of EZF. The concentrations of specnuezhenide and wedelolactone in rat kidney were 7.19 and 0.057 mg/kg, respectively. The of specnuezhenide and wedelolactone were 2.0 and 1.50 h, respectively. Their were, respectively, 30.24 ± 2.68 and 6.39 ± 0.05 μg/L. Their were 123.30 ± 2.68 and 16.56 ± 0.98 μg/Lh, respectively. Compared with the model group, the blood glucose and the 24-hour urinary protein were significantly decreased (P

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