Synthesis and characterization of PLGA/collagen composite scaffolds as skin substitute produced by electrospinning through two different approaches

Abstract

Skin damage can occur for many reasons, including burns and injuries, which in extreme cases can even lead to death. Different methods such as electrospinning are used to produce scaffolds used in skin tissue engineering. Natural and synthetic polymers were used in this method. It was observed that the use of both natural and synthetic polymers gives better results for cell culturing rather than using of each material solely. In this study, scaffolds of poly(lactic-co-glycolic acid) and collagen were prepared using coating and common solvent methods. The characteristics of samples were evaluated through scanning electron microscopy, porosimetry, mechanical testing, degradation behavior, and in vitro assays. The mechanical and biocompatibility test results of the scaffold prepared by coating method were better than the other one. However, the degradation rate of the common solvent was nearly five times more than coating sample that leads to cytotoxicity in contact with the skin cells.

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