The Effect of Glutamate Receptor Agonists on Mouse Retinal Astrocyte [Ca2+]i

Calcium-imaging techniques were used to determine if mouse retinal astrocytes in situ respond to agonists of ionotropic (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, AMPA; N-methyl-D-aspartate, NMDA) and metabotropic (S-3,5-dihydroxyphenylglycine, DHPG; trans-1-amino-1,3-cyclopentanedicarboxylic acid, ACPD) glutamate receptors. In most cases we found no evidence that retinal astrocyte intracellular calcium ion concentration () increased in response to these glutamate agonists. The one exception was AMPA that increased in some, but not all, mouse retinal astrocytes in situ. However, AMPA did not increase in mouse retinal astrocytes in vitro, suggesting that the effect of AMPA in situ may be indirect.

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