Objective. High glucose- (HG-) induced neuronal cell death is responsible for the development of diabetic neuropathy. However, the effect of HG on metabolism in neuronal cells is still unclear. Materials and Methods. The neural-crest derived PC12 cells were cultured for 72 h in the HG (75 mM) or control (25 mM) groups. We used NMR-based metabolomics to examine both intracellular and extracellular metabolic changes in HG-treated PC12 cells. Results. We found that the reduction in intracellular lactate may be due to excreting more lactate into the extracellular medium under HG condition. HG also induced the changes of other energy-related metabolites, such as an increased succinate and creatine phosphate. Our results also reveal that the synthesis of glutamate from the branched-chain amino acids (isoleucine and valine) may be enhanced under HG. Increased levels of intracellular alanine, phenylalanine, myoinositol, and choline were observed in HG-treated PC12 cells. In addition, HG-induced decreases in intracellular dimethylamine, dimethylglycine, and 3-methylhistidine may indicate a downregulation of methyl group metabolism. Conclusions. Our metabolomic results suggest that HG-induced neuronal cell death may be attributed to a series of metabolic changes, involving energy metabolism, amino acids metabolism, osmoregulation and membrane metabolism, and methyl group metabolism.
from #Medicine via ola Kala on Inoreader http://ift.tt/2652Lo4
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
▼
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
-
▼
Ιουνίου
(946)
-
▼
Ιουν 19
(24)
- Micromachines, Vol. 7, Pages 104: A Robot-Assisted...
- Inorganics, Vol. 4, Pages 19: Monoanionic Tin Olig...
- Humanities, Vol. 5, Pages 47: “I Felt Like My Life...
- Micromachines, Vol. 7, Pages 105: Fabrication of D...
- Entropy, Vol. 18, Pages 229: Investigating Aging-R...
- Energies, Vol. 9, Pages 449: Variability Character...
- IJGI, Vol. 5, Pages 99: Evaluation of Deterministi...
- Atoms, Vol. 4, Pages 17: Series of Broad Resonance...
- Applied Sciences, Vol. 6, Pages 179: Output Proper...
- MCA, Vol. 21, Pages 25: Fixed Order Controller for...
- Modeling heterogeneity in the pluripotent state: A...
- SYMPOSIUM: First international conference on myositis
- Diagnosis and classification of idiopathic inflamm...
- New ways to subclassify patients with myositis
- Issue Information - Editorial Board
- Effect of Collaborative Recommender System Paramet...
- Key Molecular Mechanisms of Chaiqinchengqi Decocti...
- Immunopathological Features of Canine Myocarditis ...
- Herbal Medicines for Treating Metabolic Syndrome: ...
- The Effect of Glutamate Receptor Agonists on Mouse...
- Effects of Total Alkaloids of Sophora alopecuroide...
- High Glucose-Induced PC12 Cell Death by Increasing...
- Isolation and Identification of the Antimicrobial ...
- Assessment of Tree Leaves Flakes Mixed with Crude ...
-
▼
Ιουν 19
(24)
- ► Φεβρουαρίου (793)
Αναζήτηση αυτού του ιστολογίου
Κυριακή 19 Ιουνίου 2016
High Glucose-Induced PC12 Cell Death by Increasing Glutamate Production and Decreasing Methyl Group Metabolism
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
Alimentary Pharmacology &Therapeutics, EarlyView. https://ift.tt/2qECBIJ
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.