Publication date: Available online 21 May 2016
Source:Vaccine
Author(s): Pirada Suphaphiphat, Lynne Whittaker, Ivna De Souza, Rodney S. Daniels, Philip R. Dormitzer, John W. McCauley, Ethan C. Settembre
The global system for manufacturing seasonal influenza vaccines has been developed to respond to the natural evolution of influenza viruses, but the problem of antigenic mismatch continues to be a challenge in certain years. In some years, mismatches arise naturally due to the antigenic drift of circulating viruses after vaccine strain selection has already been made. In other years, antigenic differences between the vaccine virus and circulating viruses are introduced as part of the current system, which relies on the use of egg-adapted isolates as a starting material for candidate vaccine viruses (CVVs). Improving the current process for making vaccine viruses can provide great value. We have previously established a synthetic approach for rapidly generating influenza viruses in a vaccine-approved Madin Darby canine kidney (MDCK) cell line using novel, high-growth backbones that increase virus rescue efficiency and antigen yield. This technology also has the potential to produce viruses that maintain antigenic similarity to the intended reference viruses, depending on the hemagglutinin (HA) and neuraminidase (NA) sequences used for gene synthesis. To demonstrate this utility, we generated a panel of synthetic viruses using HA and NA sequences from recent isolates and showed by hemagglutination inhibition (HI) tests that all synthetic viruses were antigenically-like their conventional egg- or cell-propagated reference strains and there was no impact of the novel backbones on antigenicity. This synthetic approach can be used for the efficient production of CVVs that may be more representative of circulating viruses and may be used for both egg- and cell-based vaccine manufacturing platforms. When combined with mammalian cell culture technology for antigen production, synthetic viruses generated using HA and NA sequences from a non-egg-adapted prototype can help to reduce the potential impact of antigenic differences between vaccine virus and circulating viruses on vaccine effectiveness.
from #Medicine via ola Kala on Inoreader http://ift.tt/20lZGsO
via IFTTT
Αρχειοθήκη ιστολογίου
-
►
2023
(138)
- ► Φεβρουαρίου (74)
- ► Ιανουαρίου (64)
-
►
2022
(849)
- ► Δεκεμβρίου (61)
- ► Σεπτεμβρίου (74)
- ► Φεβρουαρίου (65)
-
►
2021
(2936)
- ► Δεκεμβρίου (59)
- ► Σεπτεμβρίου (180)
- ► Φεβρουαρίου (325)
-
►
2020
(1624)
- ► Δεκεμβρίου (293)
- ► Σεπτεμβρίου (234)
- ► Φεβρουαρίου (28)
-
►
2019
(13362)
- ► Δεκεμβρίου (19)
- ► Σεπτεμβρίου (54)
- ► Φεβρουαρίου (5586)
- ► Ιανουαρίου (5696)
-
►
2018
(66471)
- ► Δεκεμβρίου (5242)
- ► Σεπτεμβρίου (5478)
- ► Φεβρουαρίου (4835)
- ► Ιανουαρίου (5592)
-
►
2017
(44259)
- ► Δεκεμβρίου (5110)
- ► Σεπτεμβρίου (5105)
-
▼
2016
(7467)
- ► Δεκεμβρίου (514)
- ► Σεπτεμβρίου (1038)
-
▼
Μαΐου
(630)
-
▼
Μαΐ 22
(46)
- Clinical utility of the KAMRA corneal inlay
- Time of maximum cycloplegia after instillation of ...
- Ranibizumab in monotherapy and combined with photo...
- Segmentation and removal of fibrovascular membrane...
- Neurofibromatosis: an update of ophthalmic charact...
- Tear volume estimation using a modified Schirmer t...
- Ocular manifestations of Sturge–Weber syndrome: pa...
- The IMPACT study: a prospective evaluation of the ...
- An exploratory study evaluating the effects of mac...
- Pre- and postsynaptic effects of brimonidine on is...
- Comparison of intraocular pressure adjusted by cen...
- Comparison of visual outcomes and subjective visua...
- A randomized, double-blind, placebo-controlled stu...
- Effect of benzalkonium chloride-free latanoprost o...
- A nonrandomized, open-label study to evaluate the ...
- Risk factors for visual impairment associated with...
- Pharmacokinetics and aqueous humor penetration of ...
- Outcomes of excimer laser enhancements in pseudoph...
- Feasibility study of sustained-release travoprost ...
- Vitrectomy for center-involved diabetic macular edema
- Glycan Node Analysis: A Bottom-up Approach to Glyc...
- Light-driven Enzymatic Decarboxylation
- Assessment of Startle Response and Its Prepulse In...
- Plasma miR-10a: A Potential Biomarker for Coronary...
- Two-Year Follow-Up after Contact Force Sensing Rad...
- The Utility of a Digital Virtual Template for Juni...
- A Review of Animal Models of Intervertebral Disc D...
- Volatiles from Subtropical Convolvulaceae That Int...
- Unveiling alterative splice diversity from human o...
- Proteomic characterization of Nα- and Nε-acetylati...
- A proteomic signature of ovarian cancer tumor flui...
- Transcriptomic and proteomic analyses provide new ...
- A cost-effective method to get insight into the pe...
- Screening for target toxins of the antiophidic pro...
- Oncogenic KIT mutations in different exons lead to...
- From great kivas to great houses: Community format...
- Antigenic characterization of influenza viruses pr...
- A national examination of pharmacy-based immunizat...
- Report of the Cent Gardes HIV Vaccines Conference....
- The impact of implementing a demand forecasting sy...
- Humoral, T-cell and B-cell immune responses to sea...
- Effectiveness of seasonal trivalent influenza vacc...
- Ultrastructural Changes and Death of Leishmania in...
- Responding to the existentials of non-life-threate...
- Home-applied IPL epilation may prevent the problem...
- General vulnerability to stuttering: The experienc...
-
▼
Μαΐ 22
(46)
- ► Φεβρουαρίου (793)
Αναζήτηση αυτού του ιστολογίου
Εγγραφή σε:
Σχόλια ανάρτησης (Atom)
-
Αλέξανδρος Γ. Σφακιανάκης Medicine by Alexandros G. Sfakianakis,Anapafseos 5 Agios Nikolaos 72100 Crete Greece,00302841026182,0030693260717...
-
heory of COVID-19 pathogenesis Publication date: November 2020Source: Medical Hypotheses, Volume 144Author(s): Yuichiro J. Suzuki ScienceD...
-
Alimentary Pharmacology &Therapeutics, EarlyView. https://ift.tt/2qECBIJ
Δεν υπάρχουν σχόλια:
Δημοσίευση σχολίου
Σημείωση: Μόνο ένα μέλος αυτού του ιστολογίου μπορεί να αναρτήσει σχόλιο.